Additional file 5: of Detection of mammalian orthoreovirus type-3 (Reo-3) infections in mice based on serotype-specific hemagglutination protein sigma-1

Excel Sheet Raw data of ELISA validation. Sheet 1: Raw data of repeatability, intermediate precision and calculation of the limit of detection. Sheet 2: Raw data of stability experiments. Sheet 3: Raw data of ROC analysis. (XLSX 21 kb

Additional file 4: of Detection of mammalian orthoreovirus type-3 (Reo-3) infections in mice based on serotype-specific hemagglutination protein sigma-1

Temperature-dependent stability of Strep-rσ-1-His-ELISA. Freshly coated ELISA plates were stored at 4 °C (black) and 37 °C (red), respectively, including all material needed for testing (controls, buffers, conjugate, substrate, sulfuric acid). Positive control (solid line, anti-His antibody) and negative control (dashed line, pool of reovirus type-3 [−] sera) were assayed in replicates of three at a total of nine time points for each storage temperature. Mean values and standard deviations are indicated. (TIF 178 kb

Additional file 1: of Detection of mammalian orthoreovirus type-3 (Reo-3) infections in mice based on serotype-specific hemagglutination protein sigma-1

Alignment of σ-1 amino acid sequences shows conservation within and diversity across reovirus serotypes. The following GenBank accession numbers were used for the BLASTp alignment: HM159619.1 (T3D), GU589583.1 (T3A), M35964.1 (T2J) and M35963.1 (T1L). Absolute and percentage values of identity, positives and gaps as well as percentage of query cover are provided. Information modified after [36]. (XLSX 11 kb