44 research outputs found

    Models of the three-dimensional structure of WBP1.

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    <p><b>A</b>: variant T, corresponding to the major allele; <b>B</b>: variant P corresponding to the minor allele; <b>C</b>: Superimposed models of both isoforms of the protein. In each panel, the light color side chains represent the position of the AA affected by the SNP.</p

    Effects of a <i>WBP1</i> antisense oligonucleotide GapmeR on total cell number and intensity of immunoreactive nuclear CDX2 in the bovine blastocyst.

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    <p><b>A</b>. Inner cell mass number <b>B.</b> trophectoderm cell number and <b>C</b>. Total cell number in blastocyst at Day 8 after insemination. Data are least-squares means ± SEM of cell number. <b>D.</b> Immunolocalization of CDX2 measured by net intensity. Data are LSM ± SEM of arbitrary intensity units. In each panel means with different superscripts differ from each other (P<0.05).</p

    Characteristics of the SNP in <i>WBP1</i> associated with embryonic development.

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    <p>Characteristics of the SNP in <i>WBP1</i> associated with embryonic development.</p

    Effects of a <i>WBP1</i> antisense oligonucleotide GapmeR on <i>WBP1</i> expression and development of the embryo to the blastocyst stage.

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    <p><b>A</b>. Transcript abundance for <i>WBP1</i> expression in 9–16 cell preimplantation embryos at 72–76 hpi. Data are least-squares means ± SEM of fold-change values relative to the vehicle treatment. <b>B</b> and <b>C</b>. Development at Day 7 (B), and Day 8 (C) of development. Data are least-squares means ± SEM of percent of putative zygotes that reached the blastocyst stage.</p

    The 20 loci with the largest proportion of SNP variance explained for rectal temperature using 3-SNP sliding windows.

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    <p>The 20 loci with the largest proportion of SNP variance explained for rectal temperature using 3-SNP sliding windows.</p

    The 20 loci with the largest proportion of SNP variance explained for rectal temperature using 5-SNP sliding windows.

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    <p>The 20 loci with the largest proportion of SNP variance explained for rectal temperature using 5-SNP sliding windows.</p

    Proportion of SNP variance explained by 3-SNP moving windows for rectal temperature from a single-step GBLUP analysis.

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    <p>Proportion of SNP variance explained by 3-SNP moving windows for rectal temperature from a single-step GBLUP analysis.</p

    Patterns of expression of genes during preimplantation period.

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    <p>Data are least-squares means ± SEM of fold-change values relative to the geometric mean of three housekeeping genes. Stage of development affected gene expression for <i>IRF9</i> (P<0.001) and other genes (P<0.0001). blast.: blastocyst. Means with different superscripts differ from each other (P<0.05).</p

    Characteristics of candidate genes associated with embryonic development in the cow: Evidence for a role for <i>WBP1</i> in development to the blastocyst stage

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    <div><p>The goal was to gain understanding of how 12 genes containing SNP previously related to embryo competence to become a blastocyst (<i>BRINP3</i>, <i>C1QB</i>, <i>HSPA1L</i>, <i>IRF9</i>, <i>MON1B</i>, <i>PARM1</i>, <i>PCCB</i>, <i>PMM2</i>, <i>SLC18A2</i>, <i>TBC1D24</i>, <i>TTLL3</i> and <i>WBP1</i>) participate in embryonic development. Gene expression was evaluated in matured oocytes and embryos. <i>BRINP3</i> and <i>C1QB</i> were not detected at any stage. For most other genes, transcript abundance declined as the embryo developed to the blastocyst stage. Exceptions were for <i>PARM1</i> and <i>WBP1</i>, where steady-state mRNA increased at the 9–16 cell stage. The SNP in <i>WBP1</i> caused large differences in the predicted three-dimensional structure of the protein while the SNP in <i>PARM1</i> caused smaller changes. The mutation in <i>WBP1</i> causes an amino acid substitution located close to a P-P-X-Y motif involved in protein-protein interactions. Moreover, the observation that the reference allele varies between mammalian species indicates that the locus has not been conserved during mammalian evolution. Knockdown of mRNA for <i>WBP1</i> decreased the percent of putative zygotes becoming blastocysts and reduced the number of trophectoderm cells and immunoreactive CDX2 in the resulting blastocysts. <i>WBP1</i> is an important gene for embryonic development in the cow. Further research to identify how the SNP in <i>WBP1</i> affects processes leading to differentiation of the embryo into TE and ICM lineages is warranted.</p></div

    Average (±SE) and range of animal and environmental variables used in the analysis.<sup>a</sup>

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    <p>Average (±SE) and range of animal and environmental variables used in the analysis.<sup>a</sup></p
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