Common allelic variants of the vitamin receptor D gene rs7975232 (Apai) do not influence bone mineral density figures in postmenopausal osteoporotic women

Este estudio examina la asociación entre la densidad mineral ósea (DMO) y el polimosfirmo rs7975232 (ApaI) del gen receptor de la vitamina D (VDR). El polimorfismo es detectado mediante un PCR en tiempo real por el método TaqMan. El genotipo rs7975232, se determinó en 274 fracturas osteoporóticas en mujeres españolas posmenopáusicas, con 60.53±8.02 años. Las frecuencias genotípicas observadas estaban de acuerdo con el equilibrio de Hardy-Weinberg (χ2=1.85, p=0.1736). No hubo diferencias significativas en el grupo genotipo rs7975232 de la muestra total de fracturas osteoporóticas por mujeres en edad, años después de la menopausia, la altura, el peso y la DMO en el cuello femoral, trochanter femoral y columna lumbar. Se encontraron diferencias significativas en la edad de la menarca (aa vs Aa; P=0,008) y el IMC (aa vs AA; P=0.029). Concluimos que el gen VDR del polimorfismo rs7975232 no está relacionado con las cifras de la densidad mineral ósea en fracturas osteoporóticas en mujeres españolas.This study examined the association between bone mineral density (BMD) and the rs7975232 (ApaI) poly¬morphism of the vitamin receptor D (VDR) gene. The polymorphism was detected using the real-time PCR TaqMan method. The rs7975232 genotype was determined in 274 postmenopausal osteoporotic Spanish women who were 60.53±8.02 years old. The observed genotype frequencies were in agreement with Hardy-Weinberg equilibrium (χ2=1.85, P=0.1736). There were no significant differences in the rs7975232 genotype groups in our total sample of osteoporotic women regarding age, years since menopause, height, weight, and BMD at femoral neck, femoral trochanter and lumbar spine. Significant differences were found in menarche age (aa vs Aa; P=0.008) and BMI (aa vs AA; P=0.029). We conclude that the VDR gene rs7975232 polymorphism is not related to figures of bone mineral density in postmenopausal osteoporotic Spanish women.peerReviewe

Lack of influence of vitamin D receptor bsmI (rs1544410) polymorphism on the rate of bone loss in a cohort of postmenopausal Spanish women affected by osteoporosis and followed for five years

Se ha realizado un estudio longitudinal para investigar la relación entre un polimorfismo en el gen receptor de la vitamina D (VDR) y los cambios en la densidad mineral ósea (DMO) y ultrasonido cuantitativo de las falanges (QUS) durante un período de cinco años. Los sujetos fueron 456 mujeres posmenopáusicas con osteoporosis en tratamiento, de edades comprendidas entre los 59,95±7,97 años (media ± desviación estándar [SD]) en la línea de base. Se midió la DMO en columna lumbar y la cadera mediante absorciometría de rayos X de doble energía y el QUS fue medido por medio de una velocidad que depende de la amplitud del sonido Ad SoS en las falanges. La información de estilo de vida se obtiene a través de un cuestionario. Las frecuencias de los genotipos BsmI (rs1544410) polimorfismo genético fueron 29,4%, 47,1% y 23,5%para bb, BB y BB, respectivamente. Después de cinco años, la DMO (variación anual en %/año) en el cuello femoral (FN) mostraron una modificación significativa sobre la base de la rs1544410 genotipo (BB vs Bb); hubo una disminución general de la masa ósea (0,70±2,79%/año; P = 0,025). Un análisis de covarianza con ajustes para la edad, peso, altura, porcentaje de cambio de peso por año, previsto la DMO y la ingesta de calcio mostraron que las asociaciones observadas ya no fueron significativas (P = 0,429). No se hallaron asociaciones significativas entre las mediciones QUS y el genotipo rs1544410 después del período de cinco años. Las limitaciones de nuestro estudio incluyen la falta de información sobre el tipo y la duración de la duración del tratamiento de la osteoporosis. Nuestros resultados indican que los polimorfismos rs1544410 no cuentan significativamente por los cambios en la masa ósea en mujeres españolas con osteoporosis en tratamiento.A longitudinal study was conducted to investigate the relation between a polymorphism in the vitamin D receptor (VDR) gene and changes in bone mineral density (BMD) and quantitative ultrasound of the phalanges (QUS) over a five-year period. The subjects were 456 postmenopausal women with steoporosis undergoing treatment, aged 59.95±7.97 years (mean ± standard deviation [SD]) at baseline. BMD was measured at the hips and lumbar spine by dual energy X-ray absorptiometry, and QUS was measured by means of amplitude-dependent speed of sound (Ad SoS) at the phalanges. Lifestyle information was obtained via a questionnaire. The genotype frequencies of the BsmI (rs1544410) gene polymorphism were 29.4%, 47.1%, and 23.5%for bb, Bb, and BB, respectively. After five years, BMD (annual change in %/year) at the femoral neck (FN) showed a significant modification based on the rs1544410 genotype (BB vs Bb); there was an overall decrease in bone mass (-0.70±2.79%/year; P = 0.025). An analysis of covariance with adjustments for age, weight, height, percentage of weight change per year, baseline BMD and calcium intake showed that the observed associations were no longer significant (P = 0.429). No significant associations were found between the QUS measurements and the rs1544410 genotype after the five-year period. Our study limitations includes lack of information about type and length of duration of the osteoporosis treatment. Our results indicate that rs1544410 polymorphisms do not account ignificantly for the changes in bone mass in Spanish women with osteoporosis undergoing treatment.peerReviewe

Lack of association of vitamin D receptor BsmI gene polymorphism with bone mineral density in Spanish postmenopausal women

La osteoporosis es un trastorno poligénico que está determinada por los efectos de varios genes, cada uno con efectos relativamente modestos en la masa ósea. El objetivo de este estudio es determinar si el receptor de la vitamina D, único nucleótido polimorfismo BsmI, está asociado con la densidad mineral ósea (DMO) de mujeres posmenopáusicas españolas. Un total de 210 mujeres posmenopáusicas sanas, mayores de 60 ± 8 años, fueron genotipificadas utilizando ensayos de genotipado SNP TaqMan® lumbar y la DMO femoral; y se determinó por la absorciometría de rayos X de energía dual (DEXA) la cantidad diaria de calcio y vitamina D. No se encontraron diferencias en el cuello femoral, trocánter, triángulo de salas, L2, L3, L4, L2-L4, o entre el cuello femoral y la DMO de cadera total tras el ajuste para posibles factores de confusión (P > 0,05) (edad, IMC, años después de la menopausia y la ingesta diaria de calcio). La BsmI polimorfismo en el gen VDR no se asoció con la DMO en mujeres posmenopáusicas españolas.Osteoporosis is a polygenic disorder that is determined by the effects of several genes, each with relatively modest effects on bone mass. The aim of this study was to determine whether the vitamin D receptor single nucleotide polymorphism BsmI is associated with bone mineral density (BMD) in Spanish postmenopausal women. A total of 210 unrelated healthy postmenopausal women aged 60 ± 8 years were genotyped using TaqManR SNP Genotyping Assays. Lumbar and femoral BMD were determined by dual-energy X-ray absorptiometry (DEXA). Daily calcium and vitamin D intake were determined by a food questionnaire. No differences were found in the femoral neck, trochanter, Ward’s Triangle, L2, L3, L4, L2-L4, or between the femoral neck and total hip BMD after further adjustment for potential confounding factors (P > 0.05) (age, BMI, years since menopause and daily calcium intake). The BsmI polymorphism in the VDR gene was not associated with BMD in Spanish postmenopausal women.peerReviewe

Simodont: Creación de biblioteca virtual mediante la incorporación de nuevos casos de cariology en entorno simulado

Depto. de Odontología Conservadora y PrótesisFac. de OdontologíaFALSEsubmitte

Cognitive Reserve Characteristics and Occupational Performance Implications in People with Mild Cognitive Impairment

The Cognitive Reserve hypothesis suggests that there are individual differences in the ability to cope with the pathologic changes in Alzheimer’s Disease. The proportion of elderly individuals has increased in recent years; this increase emphasizes the importance of early detection of mild cognitive impairment and the promotion of healthy ageing. The purpose of our study is to characterize cognitive reserve and occupational performance implications in people with mild cognitive impairment. 125 patients with mild cognitive impairment were enrolled. The Montreal Cognitive Assessments (MoCA) was used to evaluate cognitive status and the Cognitive Reserve Index Questionnaire (CRIq) as an indicator of cognitive reserve. Higher level of education was associated with higher MoCA scores (r = 0.290, p = 0.001). Positive significant correlations were observed between MoCA and total CRIq (r = 0.385, p < 0.001) as well as its three sub-domains, education (r = 0.231, p = 0.010), working activity (r = 0.237, p = 0.008) and leisure time (r = 0.319, p < 0.001). This study findings provide the importance of considering socio-behavioral factors in cognitive status. This research helps to describe the importance of engaging occupationally along the whole life-course as a potential protective factor in ageing, and includes a perspective of occupational therapy regarding the hypothesis of cognitive reserve.Depto. de EnfermeríaFac. de Enfermería, Fisioterapia y PodologíaTRUEpu

Utilización de la herramienta DIAGNOdent pen en las prácticas clínicas de la asignatura Patología y Terapeútica Dental (PTD) II y su analisis mediante Inteligencia Artificial (IA)

Depto. de Odontología Conservadora y PrótesisFac. de OdontologíaFALSEsubmitte

Aplicación de técnicas de inteligencia artificial para el diagnóstico de osteoporosis mediante el análisis de ortopantomografías como ayuda a la enseñanza e investigación

Depto. de Ingeniería de Software e Inteligencia Artificial (ISIA)Fac. de InformáticaFALSEsubmitte

Diagnostico de caries mediante dos herramientas en el grado de Odontología: DIAGNOdent™ pen. e Inteligencia Artificial

Depto. de Odontología Conservadora y PrótesisFac. de OdontologíaFALSEsubmitte

Impact of COVID-19 on cardiovascular testing in the United States versus the rest of the world

Objectives: This study sought to quantify and compare the decline in volumes of cardiovascular procedures between the United States and non-US institutions during the early phase of the coronavirus disease-2019 (COVID-19) pandemic. Background: The COVID-19 pandemic has disrupted the care of many non-COVID-19 illnesses. Reductions in diagnostic cardiovascular testing around the world have led to concerns over the implications of reduced testing for cardiovascular disease (CVD) morbidity and mortality. Methods: Data were submitted to the INCAPS-COVID (International Atomic Energy Agency Non-Invasive Cardiology Protocols Study of COVID-19), a multinational registry comprising 909 institutions in 108 countries (including 155 facilities in 40 U.S. states), assessing the impact of the COVID-19 pandemic on volumes of diagnostic cardiovascular procedures. Data were obtained for April 2020 and compared with volumes of baseline procedures from March 2019. We compared laboratory characteristics, practices, and procedure volumes between U.S. and non-U.S. facilities and between U.S. geographic regions and identified factors associated with volume reduction in the United States. Results: Reductions in the volumes of procedures in the United States were similar to those in non-U.S. facilities (68% vs. 63%, respectively; p = 0.237), although U.S. facilities reported greater reductions in invasive coronary angiography (69% vs. 53%, respectively; p < 0.001). Significantly more U.S. facilities reported increased use of telehealth and patient screening measures than non-U.S. facilities, such as temperature checks, symptom screenings, and COVID-19 testing. Reductions in volumes of procedures differed between U.S. regions, with larger declines observed in the Northeast (76%) and Midwest (74%) than in the South (62%) and West (44%). Prevalence of COVID-19, staff redeployments, outpatient centers, and urban centers were associated with greater reductions in volume in U.S. facilities in a multivariable analysis. Conclusions: We observed marked reductions in U.S. cardiovascular testing in the early phase of the pandemic and significant variability between U.S. regions. The association between reductions of volumes and COVID-19 prevalence in the United States highlighted the need for proactive efforts to maintain access to cardiovascular testing in areas most affected by outbreaks of COVID-19 infection

Seguimiento longitudinal de la masa ósea en mujeres postmenopáusicas en función de los polimorfismo BSMI y APAI del gen del receptor de la vitamina D (VDR)

La Osteoporosis es una condición poligénica que está determinada por la influencia de diversos genes, cada uno de los cuales tiene un efecto modesto sobre la masa ósea. El objetivo del presente trabajo de tesis doctoral fue determinar como los polimorfismos del gen receptor de la vitamina D (BsmI y ApaI) están asociados con la densidad mineral ósea (DMO), los valores de DMO en mujeres osteoporóticas y la respuesta al tratamiento en mujeres osteoporóticas postmenopáusicas españolas. Un total de 548 mujeres participaron en las diferentes fases del estudio, y dentro de este grupo 456 fueron seguidas por un periodo de 5 años. El genotipo se determinó mediante la utilización de TaqMan® SNP Genotyping Assays. La DMO a nivel de la cadera y de la columna lumbar se determinó median absorciometría dual por rayos X. La ingesta dietética de calcio y vitamina D se determinó mediante un cuestionario. No se encontraron asociaciones con la DMO (BsmI), los valores de DMO en mujeres osteoporóticas (ApaI) y la respuesta al tratamiento (BsmI) en la muestra de estudio. Nuestros resultados indican que el posible rol de los polimorfismos estudiados en la DMO en mujeres postmenopáusicas osteoporóticas españolas debe ser cuestionada.Osteoporosis is a polygenic disorder that is determined by the effects of several genes, each with relatively modest effects on bone mass. The aim of this study was to determine whether the vitamin D receptor single nucleotide polymorphism BsmI and ApaI are associated with bone mineral density (BMD), BMD figures in osteoporosis and response to the treatment in postmenopausal osteporotic women. A total of 548 postmenopausal osteoporotic women were recruited for the study and 456 were followed for 5-years. Genotype was determined using TaqMan® SNP Genotyping Assays. Lumbar and femoral BMD were determined by dual-energy X-ray absorptiometry (DEXA). Daily calcium and vitamin D intake were determined by a food questionnaire. No associations with BMD (BsmI), BMD figures in osteoporotic women (ApaI) and response to the treatment (BsmI) were found in the studied sample. Our results indicate that the putative role of the BsmI and ApaI in the bone mineral density in postmenopausal osteporotic Spanish women should be questioned