Research and Development of an Appropriate Electric Powered Wheelchair for India

The need for assistive technology (AT) extends far outside of the countries where users have the financial capacity to buy them, or there is a social welfare infrastructure to subsidize their purchase. In developing countries, where both technology and financial resources are scarce, AT users face tremendous challenges to find high-quality devices that are affordable. This scenario is in part due to organizations who transfer sub-standard AT to these countries by taking a 'something is better than nothing approach' to a clinically and technologically challenging problem. The goal of this work is to develop and demonstrate AT design and technology transfer strategies that take into consideration the clinical and technological needs of the intended user population. Although other projects have and do take these important user-centered factors into consideration, this work represents the first comprehensive attempt from an academic perspective, where hypotheses are proposed and tested, and design goals are described and evaluated with respect to the final product. The main focus of this work is on mobility devices (wheelchairs, specifically) although many of the protocols and techniques could be extended to address AT design and technology transfer to developing countries in general. The first part of this dissertation (Chapters 1 & 2) focuses on the background literature, and proposes a model describing the important factors influencing the success or failure of a wheelchair technology transfer project. The second part of this dissertation provides a case study in the development of an electric powered wheelchair for users in India. First, an analysis of a commercially available low-cost powered wheelchairs were evaluated (Chapter 3). Second, a study to assess the needs of users in India was performed using a modified ethnographic approach (Chapter 4). Third, the design and evaluation of a novel low-cost electric powered wheelchair for Indian users is described (Chapter 5). The final chapter (Chapter 6) discusses the implication of this work and suggests future directions. The entire drawing-set for the second generation prototype developed here is also included, allowing others to build upon the approach and the design developed here

ISWP Wheelchair Double Drum Test Version 1.3

This document outlines the required materials and proper assembly instructions for the International Society of Wheelchair Professionals Standard Testing -- Wheelchair Double Drum Testing Machine in compliance with RESNA WC-1 ISO 7176 standards that require performing 200,000 revolutions at a speed of 1 m/s +/- 0.1 m/s. Following the detailed assembly instructions are a bill of materials and 2-dimensional drawings for all of the sub-assemblies and their individual parts. This document outlines the electrical requirements and recommendations to successfully build this equipment, however specific electrical components and electrical assembly instructions are to be determined by the user

ISWP Wheelchair Curb Drop Test Version 1.3

This document outlines the required materials and proper assembly instructions for the International Society of Wheelchair Professionals Standard Testing --Wheelchair Curb Drop Testing Machine in compliance with RESNA WC-1/ ISO 7176 standards that require dropping the wheelchair from a height of 50 mm for 6,666 cycles. Following the detailed assembly instructions are a bill of materials and 2-dimensional drawings for all of the sub-assemblies and their individual parts. This document outlines the electrical requirements and recommendations

Refined annotation and assembly of the Tetrahymena thermophila genome sequence through EST analysis, comparative genomic hybridization, and targeted gap closure

<p>Abstract</p> <p>Background</p> <p><it>Tetrahymena thermophila</it>, a widely studied model for cellular and molecular biology, is a binucleated single-celled organism with a germline micronucleus (MIC) and somatic macronucleus (MAC). The recent draft MAC genome assembly revealed low sequence repetitiveness, a result of the epigenetic removal of invasive DNA elements found only in the MIC genome. Such low repetitiveness makes complete closure of the MAC genome a feasible goal, which to achieve would require standard closure methods as well as removal of minor MIC contamination of the MAC genome assembly. Highly accurate preliminary annotation of <it>Tetrahymena</it>'s coding potential was hindered by the lack of both comparative genomic sequence information from close relatives and significant amounts of cDNA evidence, thus limiting the value of the genomic information and also leaving unanswered certain questions, such as the frequency of alternative splicing.</p> <p>Results</p> <p>We addressed the problem of MIC contamination using comparative genomic hybridization with purified MIC and MAC DNA probes against a whole genome oligonucleotide microarray, allowing the identification of 763 genome scaffolds likely to contain MIC-limited DNA sequences. We also employed standard genome closure methods to essentially finish over 60% of the MAC genome. For the improvement of annotation, we have sequenced and analyzed over 60,000 verified EST reads from a variety of cellular growth and development conditions. Using this EST evidence, a combination of automated and manual reannotation efforts led to updates that affect 16% of the current protein-coding gene models. By comparing EST abundance, many genes showing apparent differential expression between these conditions were identified. Rare instances of alternative splicing and uses of the non-standard amino acid selenocysteine were also identified.</p> <p>Conclusion</p> <p>We report here significant progress in genome closure and reannotation of <it>Tetrahymena thermophila</it>. Our experience to date suggests that complete closure of the MAC genome is attainable. Using the new EST evidence, automated and manual curation has resulted in substantial improvements to the over 24,000 gene models, which will be valuable to researchers studying this model organism as well as for comparative genomics purposes.</p

Macronuclear Genome Sequence of the Ciliate Tetrahymena thermophila, a Model Eukaryote

The ciliate Tetrahymena thermophila is a model organism for molecular and cellular biology. Like other ciliates, this species has separate germline and soma functions that are embodied by distinct nuclei within a single cell. The germline-like micronucleus (MIC) has its genome held in reserve for sexual reproduction. The soma-like macronucleus (MAC), which possesses a genome processed from that of the MIC, is the center of gene expression and does not directly contribute DNA to sexual progeny. We report here the shotgun sequencing, assembly, and analysis of the MAC genome of T. thermophila, which is approximately 104 Mb in length and composed of approximately 225 chromosomes. Overall, the gene set is robust, with more than 27,000 predicted protein-coding genes, 15,000 of which have strong matches to genes in other organisms. The functional diversity encoded by these genes is substantial and reflects the complexity of processes required for a free-living, predatory, single-celled organism. This is highlighted by the abundance of lineage-specific duplications of genes with predicted roles in sensing and responding to environmental conditions (e.g., kinases), using diverse resources (e.g., proteases and transporters), and generating structural complexity (e.g., kinesins and dyneins). In contrast to the other lineages of alveolates (apicomplexans and dinoflagellates), no compelling evidence could be found for plastid-derived genes in the genome. UGA, the only T. thermophila stop codon, is used in some genes to encode selenocysteine, thus making this organism the first known with the potential to translate all 64 codons in nuclear genes into amino acids. We present genomic evidence supporting the hypothesis that the excision of DNA from the MIC to generate the MAC specifically targets foreign DNA as a form of genome self-defense. The combination of the genome sequence, the functional diversity encoded therein, and the presence of some pathways missing from other model organisms makes T. thermophila an ideal model for functional genomic studies to address biological, biomedical, and biotechnological questions of fundamental importance