Impaired limb shortening following stroke: what's in a name?

BackgroundDifficulty advancing the paretic limb during the swing phase of gait is a prominent manifestation of walking dysfunction following stroke. This clinically observable sign, frequently referred to as 'foot drop', ostensibly results from dorsiflexor weakness.ObjectiveHere we investigated the extent to which hip, knee, and ankle motions contribute to impaired paretic limb advancement. We hypothesized that neither: 1) minimal toe clearance and maximal limb shortening during swing nor, 2) the pattern of multiple joint contributions to toe clearance and limb shortening would differ between post-stroke and non-disabled control groups.MethodsWe studied 16 individuals post-stroke during overground walking at self-selected speed and nine non-disabled controls who walked at matched speeds using 3D motion analysis.ResultsNo differences were detected with respect to the ankle dorsiflexion contribution to toe clearance post-stroke. Rather, hip flexion had a greater relative influence, while the knee flexion influence on producing toe clearance was reduced.ConclusionsSimilarity in the ankle dorsiflexion, but differences in the hip and knee, contributions to toe clearance between groups argues strongly against dorsiflexion dysfunction as the fundamental impairment of limb advancement post-stroke. Marked reversal in the roles of hip and knee flexion indicates disruption of inter-joint coordination, which most likely results from impairment of the dynamic contribution to knee flexion by the gastrocnemius muscle in preparation for swing. These findings suggest the need to reconsider the notion of foot drop in persons post-stroke. Redirecting the focus of rehabilitation and restoration of hemiparetic walking dysfunction appropriately, towards contributory neuromechanical impairments, will improve outcomes and reduce disability

Murine Models of Splenic Marginal Zone Lymphoma: A role for Cav1?

Dozens of murine models of indolent and aggressive B-cell lymphomas have been generated to-date. These include those manifesting chronic lymphocytic leukemia (CLL), diffuse large B-cell lymphoma (DLBCL) as well as xenografts of mantle cell lymphoma (MCL). These models have led to an improved understanding of disease etiology, B-cell biology, immunomodulation, and the importance of the tumor microenvironment. Despite these efforts in CLL, DLBCL, and MCL, considerably little progress toward a model of splenic marginal zone lymphoma (SMZL) has been accomplished. Herein, we describe the similarities and differences between CLL, MCL, and SMZL, and highlight effective murine models that mimic disease in the two former, in hopes of informing a potential model of the latter. At the time of writing this review, the precise molecular events of SMZL remain to be determined and a treatment regimen remains to be identified. Therefore, based on the efforts put forth in the B-cell lymphoma field throughout the past 3 decades, the established role of caveolin-1 in B- and T-cell biology as an oncogene or tumor suppressor, and the recurrent deletion or loss of heterozygosity (LOH) of 7q in many cancers, we make recommendations for a murine model of SMZL

fMRI Activity in Posterior Parietal Cortex Relates to the Perceptual Use of Binocular Disparity for Both Signal-In-Noise and Feature Difference Tasks.

Visually guided action and interaction depends on the brain's ability to (a) extract and (b) discriminate meaningful targets from complex retinal inputs. Binocular disparity is known to facilitate this process, and it is an open question how activity in different parts of the visual cortex relates to these fundamental visual abilities. Here we examined fMRI responses related to performance on two different tasks (signal-in-noise "coarse" and feature difference "fine" tasks) that have been widely used in previous work, and are believed to differentially target the visual processes of signal extraction and feature discrimination. We used multi-voxel pattern analysis to decode depth positions (near vs. far) from the fMRI activity evoked while participants were engaged in these tasks. To look for similarities between perceptual judgments and brain activity, we constructed 'fMR-metric' functions that described decoding performance as a function of signal magnitude. Thereafter we compared fMR-metric and psychometric functions, and report an association between judged depth and fMRI responses in the posterior parietal cortex during performance on both tasks. This highlights common stages of processing during perceptual performance on these tasks.This is the final version of the article. It first appeared from PLOS via http://dx.doi.org/10.1371/journal.pone.014069

Biomolecule Detection \u3cem\u3evia\u3c/em\u3e Target Mediated Nanoparticle Aggregation and Dielectrophoretic Impedance Measurement

A new biosensing system is described that is based on the aggregation of nanoparticles by a target biological molecule and dielectrophoretic impedance measurement of these aggregates. The aggregation process was verified within a microchannel via fluorescence microscopy, demonstrating that this process can be used in a real time sensor application. Positive dielectrophoresis is employed to capture the nanoparticle aggregates at the edge of thin film electrodes, where their presence is detected either by optical imaging via fluorescence microscopy or by measuring the change in electrical impedance between adjacent electrodes. The electrical detection mechanism demonstrates the potential for this method as a micro total analysis system (mTAS)

Rotation periods of late-type stars in the young open cluster IC 2602

We present the results of a monitoring campaign aimed at deriving rotation periods for a representative sample of stars in the young (30 Myr) open cluster IC 2602. Rotation periods were derived for 29 of 33 stars monitored. The periods derived range from 0.2d (one of the shortest known rotation periods of any single open cluster star) to about 10d (which is almost twice as long as the longest period previously known for a cluster of this age). We are able to confirm 8 previously known periods and derive 21 new ones, delineating the long period end of the distribution. Despite our sensitivity to longer periods, we do not detect any variables with periods longer than about 10d. The combination of these data with those for IC 2391, an almost identical cluster, leads to the following conclusions: 1) The fast rotators in a 30 Myr cluster are distributed across the entire 0.5 < B-V < 1.6 color range. 2) 6 stars in our sample are slow rotators, with periods longer than 6d. 3) The amplitude of variability depends on both the color and the period. The dependence on the latter might be important in understanding the selection effects in the currently available rotation period database and in planning future observations. 4) The interpretation of these data in terms of theoretical models of rotating stars suggests both that disk-interaction is the norm rather than the exception in young stars and that disk-locking times range from zero to a few Myr.Comment: 23 pages, 8 figures, accepted for publication in the Astrophysical Journa

Water Abundance in Molecular Cloud Cores

We present Submillimeter Wave Astronomy Satellite (SWAS) observations of the 1_{10}-1_{01} transition of ortho-water at 557 GHz toward 12 molecular cloud cores. The water emission was detected in NGC 7538, Rho Oph A, NGC 2024, CRL 2591, W3, W3(OH), Mon R2, and W33, and was not detected in TMC-1, L134N, and B335. We also present a small map of the water emission in S140. Observations of the H_2^{18}O line were obtained toward S140 and NGC 7538, but no emission was detected. The abundance of ortho-water relative to H_2 in the giant molecular cloud cores was found to vary between 6x10^{-10} and 1x10^{-8}. Five of the cloud cores in our sample have previous water detections; however, in all cases the emission is thought to arise from hot cores with small angular extents. The water abundance estimated for the hot core gas is at least 100 times larger than in the gas probed by SWAS. The most stringent upper limit on the ortho-water abundance in dark clouds is provided in TMC-1, where the 3-sigma upper limit on the ortho-water fractional abundance is 7x10^{-8}.Comment: 5 pages, 3 Postscript figures, uses aastex.cls, emulateapj5.sty (included), and apjfonts.sty (included

The Distribution of Water Emission in M17SW

We present a 17-point map of the M17SW cloud core in the 1_{10}-1_{01} transition of ortho-water at 557 GHz obtained with the Submillimeter Wave Astronomy Satellite. Water emission was detected in 11 of the 17 observed positions. The line widths of the water emission vary between 4 and 9 km s^{-1}, and are similar to other emission lines that arise in the M17SW core. A direct comparison is made between the spatial extent of the water emission and the ^{13}CO J = 5\to4 emission; the good agreement suggests that the water emission arises in the same warm, dense gas as the ^{13}CO emission. A spectrum of the H_2^{18}O line was also obtained at the center position of the cloud core, but no emission was detected. We estimate that the average abundance of ortho-water relative to H_2 within the M17 dense core is approximately 1x10^{-9}, 30 times smaller than the average for the Orion core. Toward the H II region/molecular cloud interface in M17SW the ortho-water abundance may be about 5 times larger than in the dense core.Comment: 4 pages, 3 Postscript figures, uses aastex.cls, emulateapj5.sty (included), and apjfonts.sty (included

Discovery of Two T Dwarf Companions with the Spitzer Space Telescope

We report the discovery of T dwarf companions to the nearby stars HN Peg (G0V, 18.4 pc, ~0.3 Gyr) and HD 3651 (K0V, 11.1 pc, ~7 Gyr). During an ongoing survey of 5'x5' fields surrounding stars in the solar neighborhood with IRAC aboard the Spitzer Space Telescope, we identified these companions as candidate T dwarfs based on their mid-IR colors. Using near-IR spectra obtained with SpeX at the NASA IRTF, we confirm the presence of methane absorption that characterizes T dwarfs and measure spectral types of T2.5+/-0.5 and T7.5+/-0.5 for HN Peg B and HD 3651 B, respectively. By comparing our Spitzer data to images from 2MASS obtained several years earlier, we find that the proper motions of HN Peg B and HD 3651 B are consistent with those of the primaries, confirming their companionship. HN Peg B and HD 3651 B have angular separations of 43.2" and 42.9" from their primaries, which correspond to projected physical separations of 795 and 476 AU, respectively. A comparison of their luminosities to the values predicted by theoretical evolutionary models implies masses of 0.021+/-0.009 and 0.051+/-0.014 Msun for HN Peg B and HD 3651 B. In addition, the models imply an effective temperature for HN Peg B that is significantly lower than the values derived for other T dwarfs at similar spectral types, which is the same behavior reported by Metchev & Hillenbrand for the young late-L dwarf HD 203030 B. Thus, the temperature of the L/T transition appears to depend on surface gravity. Meanwhile, HD 3651 B is the first substellar companion directly imaged around a star that is known to harbor a close-in planet from RV surveys. The discovery of this companion supports the notion that the high eccentricities of close-in planets like the one near HD 3651 may be the result of perturbations by low-mass companions at wide separations.Comment: Astrophysical Journal, in pres