1 research outputs found
Experimental and Theoretical Polarized Raman Linear Difference Spectroscopy of Small Molecules with a New Alignment Method Using Stretched Polyethylene Film
This paper reports the development of the new technique
of Raman
linear difference (RLD) spectroscopy and its application to small
molecules: anthracene and nucleotides adenosine-5′-monophosphate,
thymidine-5′-monophosphate, guanosine-5′-monophosphate,
and cytidine-5′-monophosphate. In this work we also present
a new alignment method for Raman spectroscopy where stretched polyethylene
films are used as the matrix. Raman spectra using light polarized
along the orientation direction and perpendicular to it are reported.
The polyethylene (PE) film spectra are consistent with powder samples
and films deposited on quartz. RLD spectra determined from the difference
of the parallel and perpendicular polarized light Raman spectra are
also reported. The equations describing RLD are derived, and RLD spectra
of anthracene and thymidine are calculated from these equations using
Density Functional Theory and assuming perfect orientation of the
samples. Because of the wealth of spectroscopic information in the
vibrational spectra of biomolecules together with our ability to calculate
spectra as a function of orientation, we conclude that RLD has the
potential to provide structural information for biological samples
that currently cannot be extracted from any other method