The process of recovery of cell membrane damage produced by the lowlevel microwave radiation

The process of cell membrane recovery after the influence of microwave radiation (f = 36.64 GHz, E = 10, 100, 400 μW/cm2, irradiation time 10 s) on human buccal epithelium cells was investigated. The stainability of cells with indigo carmine (5 mM) or trypan blue (0.5%) after 5 min of staining was investigated. It was shown irradiation to be induced decrease of percentage of unstained cells. Recovery of cell membrane permeability after microwave irradiation was shown. Cell recovery after microwave irradiation of low intensity (10 μW/cm2) was shown after 1 h. Microwave irradiation of higher intensity (100, 400 μW/cm2) induced more strongly effects of cell membrane permeability. Cell recovery after microwave irradiation of higher intensity was shown after 3 h. For the first time recovery of cell membrane after microwave irradiation of low intensity was shown

Simulation of microwave exposure of human cells by electromagnetic field of EMF band

To implement a novel method of investigation of electromagnetic field action on state of cell nucleus the simulation of irradiation of the cells of human buccal epithelium by electromagnetic field of EMF band is carried out. The cells were exposed to radiation of frequency f = 36.64 GHz, with power densities W = 10–1, 1 and 4 W/m2during 10 seconds. To answer on the question of existence of nonthermal biological effect of electromagnetic field onto living cells the specific absorption rate (SAR) of a solution that contained cells is calculated. The numerical simulation based on FDTD method permitted to take into account different parts of experimental setup to obtain real distribution of electromagnetic field inside irradiated solution

Drosophila melanogaster viability and mutability under the influence of low energy microwave monochromatic and ultra wideband impulse field

The influence of microwave radiation (int = 35 GHz, intensity P = 30, 70, 180, 265 mW /cm2) and ultra wideband irradiation at irradiation intensity of 10-5, 10-4, 10-3, and 10-2 W/cm2 on stocks of Drosophila melanogaster was investigated. Irradiation was performed on the stage of egg < exposure time was 10 sec... Irradiation by monochromatic radiation (35 GHz) of intensity 70, 180 and 265 mW/cm2 negatively influences upon the viability of Drosophila. No viability decrease registered after ultra wideband irradiation. Monochromatic microwave radiation induced dominant lethal mutations (DLM). The effect of radiation depends on state of polarizations of radiation. Linear polarized radiation and right circular polarized radiation induce more DLM than left circular polarized radiation. Ultra wideband .impulse radiation induced no significant decrease of Drosophila viability. Incidence of DLM increased after ultra wideband impulse irradiation at the intensity 10-4 and 10-2 W/cm2

Effects of differently polarized microwave radiation on the microscopic structure of the nuclei in human fibroblasts

To investigate the influence of microwave radiation on the human fibroblast nuclei, the effects of three variants of electromagnetic wave polarization, linear and left-handed and right-handed elliptically polarized, were examined. Experimental conditions were: frequency (f) 36.65 GHz, power density (P) at the surface of exposed object 1, 10, 30, and 100 µW/cm2, exposure time 10 s. Human fibroblasts growing in a monolayer on a cover slide were exposed to microwave electromagnetic radiation. The layer of medium that covered cells during microwave exposure was about 1 mm thick. Cells were stained immediately after irradiation by 2% (w/v) orcein solution in 45% (w/v) acetic acid. Experiments were made at room temperature (25 °C), and control cell samples were processed in the same conditions. We assessed heterochromatin granule quantity (HGQ) at 600× magnification. Microwave irradiation at the intensity of 1 µW/cm2 produced no effect, and irradiation at the intensities of 10 and 100 µW/cm2 induced an increase in HGQ. More intense irradiation induced more chromatin condensation. The right-handed elliptically polarized radiation revealed more biological activity than the left-handed polarized one