3 research outputs found
Herança da resistĂȘncia em feijoeiro Ă murcha causada por Curtobacterium flaccumfaciens pv. flaccumfaciens
Genetic mapping of quantitative trait loci (QTLs) with effects on resistance to flower bud thrips (megalurothrips sjostedti) identified in recombinant inbred lines of cowpea (Vigna unguiculata (L.) Walp)
Open Access JournalThe first major insect pest of cowpea at reproductive stage is the flower bud thrips (FTh), which, if not
controlled, is capable of causing significant grain yield reduction. Breeding for resistance to FTh in
cowpea has been hindered by the quantitative nature of the resistance, and the breakdown of
resistance under high insect infestation. The purpose of this study was to use molecular markers to
identify genetic loci associated with the expression of resistance to FTh. A set of 92 recombinant inbred
lines (RILs) was generated from a cross between susceptible and resistant lines. One hundred and
thirty nine markers [134 Amplified Fragment Length Polymorphism (AFLP) and 5 cowpea derived
microsatellites] were used to construct a linkage map using this set of RILs. The linkage map spans
1620 cM of the cowpea genome and markers were distributed in 11 linkage groups. Average distance
between adjacent markers was 9.6 cM. There were significant associations between 23 DNA markers
and resistance to flower bud thrips (P<0.05) using single marker analysis. QTLs with effects on
resistance were detected in five linkage groups. The QTL on linkage group 3 explained 32.0% of the
variation for resistance while all the five QTLs together explained 77.5%
Genetic analysis of resistance to flower bud thrips (Megalurothrips sjostedti) in cowpea (Vigna unguiculata [L.] Walp.)
Cowpea is an important legume in sub-Saharan Africa where its protein rich grains are consumed. Insect pests constitute a major constraint to cowpea production. Flower bud thrips (FTh) is thefirst major pest of cowpea at the reproductive stage and if not controlled with insecticides is capable of reducing grain yield significantly. Information on the inheritance of resistance to FTh is required to facilitate breeding of resistant cultivars. The genetics
of resistance was studied in crosses of four cowpea lines. Maternal effect was implicated while frequency distributions of the F2 and backcross generations suggest quantitative inheritance. Additive, dominance and epistatic gene effects made large contributions
and since improved inbred lines are the desired product, selection should not be too severe in the early generations to allow for desirable gene recom-bination. This study suggested that some of the genes involved in the control of resistance to FTh are different in TVu1509 and Sanzi. Broad sense heritability ranged from 56% to 73%. Choice of maternal parent in a cross will be critical to the success of resistance breeding