5 research outputs found
Bibliografie české lingvistiky v číslech a v kontextu srbocharvátsko-českých vztahů
Abstrakt referatu przygotowanego na posiedzenie bloku tematycznego Komisji Bibliografii Lingwistycznej przy Międzynarodowym Komitecie Slawistów nt. "Bibliografia językoznawstwa slawistycznego w przestrzeni cyfrowej" podczas XVI Międzynarodowego Kongresu Slawistów (Belgrad, 20-27.08.2018)
Bibliografie české lingvistiky v číslech a v kontextu srbocharvátsko-českých vztahů
Rozszerzony abstrakt referatu przygotowanego na posiedzenie bloku tematycznego Komisji Bibliografii Lingwistycznej przy Międzynarodowym Komitecie Slawistów nt. "Bibliografia językoznawstwa slawistycznego w przestrzeni cyfrowej" podczas XVI Międzynarodowego Kongresu Slawistów (Belgrad, 20-27.08.2018)
Lichens-a new source or yet unknown host of herbaceous plant viruses?
Abstract Lichens are symbiotic associations of fungi with green algae or cyanobacteria. They have arisen independently several times within the Ascomycota and Basidiomycota. This symbiosis became with time one of the most successful life forms on Earth. Outside of the symbiotic algae and fungi, there are endophytic fungi, other algae, and lichen-associated bacteria present within lichen thalli. Till now, no lichen-specific pathogens have been reported among bacteria and viruses. Around 15 dsRNA viruses are known from Eurotiomycetes and another dsRNA and reverse transcribed ssRNA viruses from Dothideomycetes containing some lichenized fungal lineages. Algal viruses have been identified from less than 1 % of known eukaryotic algal species but no virus has been found in Trebouxia or in Trentepohlia (Chlorophyta, Pleurastrophyceae, Pleurastrales), the most common green lichen photobionts. On the other hand, dsDNA viruses infecting related Chlorella algae are well known from freshwater phytoplankton. However, high-molecular weight dsRNA isolated from different lichen thalli indicated to us presence of ss or dsRNA viruses. A PCRbased search for viruses with genus-specific and speciesspecific primers resulted in amplification of genome segments highly identical with those of plant cytorhabdoviruses and with Apple mosaic virus (ApMV). The nucleotide sequence of the putative lichen cytorhabdovirus showed high identity (98 %) with Ivy latent cytorhabdovirus. The nucleotide sequences of six Apple mosaic virus isolates from lichens showed high similarity with ApMV isolates from apple and pear hosts. The lichen ApMV isolates were mechanically transmitted to an herbaceous host and detected positive in ELISA 14 days thereafter, which support its infectivity on plants. We prepared axenic cultures of photobionts identified as Trebouxia sp. from this ApMVpositive lichen samples. All these cultures were positive for ApMV in RT-PCR test. We suggest that lichens as a whole (or their photobionts, more specifically) could serve as reservoirs for viruses, despite the fact that the way of transmission between different organisms is not Eur J Plant Patho