Physical and functional interactions between STAT3 and Kaposi's Sarcoma-associated Herpesvirus-encoded LANA

The Kaposi’s sarcoma-associated herpesvirus (KSHV)-encoded latency-associated nuclear antigen (LANA) is known to modulate viral and cellular gene expression. We show that LANA directly associates with an IL-6 signal transducer, STAT3 and that LANA enhances the transcriptional activity of STAT3. Coimmunoprecipitation studies documented a physical interaction between LANA and STAT3 in transiently transfected 293T cells as well as the KSHV-infected primary effusion lymphoma (PEL) cell line. Furthermore, small-interfering RNA-mediated reduction of LANA expression decreased the STAT3-dependent transcription in KSHV-positive PEL cells, whereas overexpression of LANA enhanced STAT3 activity in KSHV-negative B lymphoma cells. These data demonstrate that LANA is a transcriptional co-activator of STAT3, and may have implications for the pathogenesis of KSHV-associated diseases

未治療の糖尿病に合併した鼻中隔膿瘍の2症例

我々は2例の鼻中隔膿瘍症例を経験した.2例とも未治療の糖尿病を合併していた.糖尿病を合併した鼻中隔膿瘍は重篤な合併症を引き起こす可能性があり,注意を要する.We encountered two cases of nasal septal abscess in patients with uncontrolled diabetes mellitus. When a diabetic patient presents with bilateral nasal obstruction, it is important to remember the possibility of nasal septal abscess

未治療の糖尿病に合併した鼻中隔膿瘍の2症例

我々は2例の鼻中隔膿瘍症例を経験した.2例とも未治療の糖尿病を合併していた.糖尿病を合併した鼻中隔膿瘍は重篤な合併症を引き起こす可能性があり,注意を要する.We encountered two cases of nasal septal abscess in patients with uncontrolled diabetes mellitus. When a diabetic patient presents with bilateral nasal obstruction, it is important to remember the possibility of nasal septal abscess

Physical and functional interactions between STAT3 and EBNA2

The Epstein-Barr virus (EBV)-encoded latency protein EBNA2 is a nuclear transcriptional activator that is essential for EBV-induced cellular transformation. Here, we show that EBNA2 interacts with STAT3, a signal transducer for an interleukin-6 family cytokine, and enhances the transcriptional activity of STAT3 by influencing its DNA-binding activity. Furthermore, EBNA2 cooperatively acts on STAT3 activation with LMP1. These data demonstrate that EBNA2 acts as a transcriptional coactivator of STAT3

Kaposi's sarcoma-associated herpesvirus-encoded LANA associates with glucocorticoid receptor and enhances its transcriptional activities

Kaposi's sarcoma-associated herpesvirus (KSHV)-encoded latency-associated nuclear antigen (LANA), which interacts with cellular proteins, plays a central role in modification of viral and/or cellular gene expression. Here, we show that LANA associates with glucocorticoid receptor (GR), and that LANA enhances the transcriptional activity of GR. Co-immunoprecipitation revealed a physical interaction between LANA and GR in transiently transfected 293T and HeLa cells. In human B-lymphoma cells, LANA overexpression enhanced GR activity and cell growth suppression following glucocorticoid stimulation. Furthermore, confocal microscopy showed that activated GR was bound to LANA and accumulated in the nucleus, leading to an increase in binding of activated GR to the glucocorticoid response element of target genes. Taken together, KSHV-derived LANA acts as a transcriptional co-activator of GR. Our results might suggest a careful use of glucocorticoids in the treatment of patients with KSHV-related malignancies such as Kaposi's sarcoma, primary effusion lymphoma, and multicentric Castleman disease. (C) 2015 Elsevier Inc. All rights reserved

A prophylactic approach to postoperative delirium through collaborative practice

We launched our Psychiatry Liaison Team in the Department of Neuropsychiatry of Okayama University Hospital in 2009. Since nearly half of all new cases had suffered delirium, we subsequently launched our Delirium Control Team in 2011. The main purposes of the Delirium Control Team are to prevent the occurrence of delirium through early interventions by various medical staff, and to educate co-medical staff about delirium care. In this article, we present the recent activities and future outlook of both the Psychiatry Liaison Team and the Delirium Control Team