Alien Registration- O\u27Toole, Mary (Portland, Cumberland County)

https://digitalmaine.com/alien_docs/24195/thumbnail.jp

Alien Registration- O\u27Toole, Mary A. (Portland, Cumberland County)

https://digitalmaine.com/alien_docs/24196/thumbnail.jp

Coming to a Full Stop: An Investigation of Menstrual Period Stigmas in College Students

The focus of this study was to understand perceptions college students have regarding menstruation and if viewing a normalized media message may influence these perceptions. A treatment group of participants was randomly assigned to view an episode of the hit family sitcom Blackish that focused on menstruation and then answer survey questions. A control group of participants only answered the survey questions. A mixed methods analysis revealed three primary conclusions. First, these results contradict existing research in that the college students surveyed generally do not hold negative perceptions that may stigmatize menstruation. Second, the treatment that viewed the normalized media message intervention did not report significantly more positive perceptions about menstruation as a natural bodily function than their counterparts in the control group. Third, many participants acknowledged menstruation is a stigmatized topic and media messages not only currently contribute to these attitudes but could be used as a catalyst for guiding society toward normalizing it. These results extend existing research on how people perceive menstruation and on mass media effect research as a means to address stigmatized topics

Alien Registration- O\u27Toole, Mary (Portland, Cumberland County)

https://digitalmaine.com/alien_docs/24195/thumbnail.jp

Lentiviral vector mediated modification of mesenchymal stem cells & enhanced survival in an in vitro model of ischaemia

Introduction: A combination of gene and cell therapies has the potential to significantly enhance the therapeutic value of mesenchymal stem cells (MSCs). The development of efficient gene delivery methods is essential if MSCs are to be of benefit using such an approach. Achieving high levels of transgene expression for the required period of time, without adversely affecting cell viability and differentiation capacity, is crucial. In the present study, we investigate lentiviral vector-mediated genetic modification of rat bone-marrow derived MSCs and examine any functional effect of such genetic modification in an in vitro model of ischaemia. Methods: Transduction efficiency and transgene persistence of second and third generation rHIV-1 based lentiviral vectors were tested using reporter gene constructs. Use of the rHIV-pWPT-EF1-alpha-GFP-W vector was optimised in terms of dose, toxicity, cell species, and storage. The in vivo condition of ischaemia was modelled in vitro by separation into its associated constituent parts i.e. hypoxia, serum and glucose deprivation, in which the effect of therapeutic gene over-expression on MSC survival was investigated. Results: The second generation lentiviral vector rHIV-pWPT-EF1-alpha-GFP-W, was the most efficient and provided the most durable transgene expression of the vectors tested. Transduction with this vector did not adversely affect MSC morphology, viability or differentiation potential, and transgene expression levels were unaffected by cryopreservation of transduced cells. Over-expression of HSP70 resulted in enhanced MSC survival and increased resistance to apoptosis in conditions of hypoxia and ischaemia. MSC differentiation capacity was significantly reduced after oxygen deprivation, but was preserved with HSP70 over-expression. Conclusions: Collectively, these data validate the use of lentiviral vectors for efficient in vitro gene delivery to MSCs and suggest that lentiviral vector transduction can facilitate sustained therapeutic gene expression, providing an efficient tool for ex vivo MSC modification. Furthermore, lentiviral mediated over-expression of therapeutic genes in MSCs may provide protection in an ischaemic environment and enable MSCs to function in a regenerative manner, in part through maintaining the ability to differentiate. This finding may have considerable significance in improving the efficacy of MSC-based therapies