Construction and Characterisation of Auxotrophic Mutants of Salmonella Typhimurium as Live Vaccines in the Murine Model of Typhoid Fever

Imperial Users onl

Evaluation of Milking Systems in Terms of Mastitis Risk, Teat Tissue Reactions & Milking Performance.

End of Project ReportsMeasurements of milking vacuum recorded on a flow simulator can provide guidelines for optimum design of milking units. • Increasing the bore of the short milk tube above the recommended diameter or claw volume above 150ml does not improve milking efficiency. • Increasing the long milk-tube bore from 13.5mm to 16mm increased the level of milking vacuum. • The milking vacuum was highest with wide-bore tapered liners and simultaneous pulsation. • The minimum vacuum was increased with narrow-bore liners and alternate pulsation. • The milk yield with wide-bore tapered liners in heavy 3-kg clusters and using simultaneous pulsation was 5% higher than with light clusters (1.65 kg) with alternate pulsation. • The milk yield depressions obtained with light clusters were similar in short and long term experiments and increased with the magnitude of the milk yield per milking. • The teat condition scores were not affected by the magnitude of vacuum fluctuations

A Feature Selection Method for Driver Stress Detection Using Heart Rate Variability and Breathing Rate

Driver stress is a major cause of car accidents and death worldwide. Furthermore, persistent stress is a health problem, contributing to hypertension and other diseases of the cardiovascular system. Stress has a measurable impact on heart and breathing rates and stress levels can be inferred from such measurements. Galvanic skin response is a common test to measure the perspiration caused by both physiological and psychological stress, as well as extreme emotions. In this paper, galvanic skin response is used to estimate the ground truth stress levels. A feature selection technique based on the minimal redundancy-maximal relevance method is then applied to multiple heart rate variability and breathing rate metrics to identify a novel and optimal combination for use in detecting stress. The support vector machine algorithm with a radial basis function kernel was used along with these features to reliably predict stress. The proposed method has achieved a high level of accuracy on the target dataset.Comment: In Proceedings of the 15th International Conference on Machine Vision (ICMV), Rome, Italy, 18-20 November 2022. arXiv admin note: text overlap with arXiv:2206.0322

Numerical optimization of hybrid dielectric/HTS resonators for surface impedance evaluation of HTS films

This work describes an alternative to the traditional dielectric resonator topology used for measuring surface impedance in high temperature superconducting (HTS) films. A gap is introduced above the dielectric so that only the lower film is in direct contact with it. This arrangement has been used extensively for mechanical tuning of dielectric resonators and, when used for surface impedance measurement, it can be designed to make the losses in the upper film small relative to the overall resonator losses. Then, measured results are mostly due to one of the films and not the average of two. The specifics of a resonator design for measuring 2-inch wafers are presented. An analysis and optimization of the resonator is done using a numerically efficient mode-matching algorithm.Peer ReviewedPostprint (published version

Latitudinal and cross-shelf patterns of size, age, growth, and mortality of a tropical damselfish Acanthochromis polyacanthus on the Great Barrier Reef

Patterns of age and growth of a sedentary damsel fish Acanthochromis polyacanthus were tested over a latitudinal range of approximately 10 degrees (1200 km) on the Great Barrier Reef (GBR), Australia. Within latitudes, these patterns were also compared on reefs in distance strata (inner, mid, and outer) across a continental shelf that ranged in width from 52 to 128 km. Although variation in length-max (SLMAX), growth, age-max (AMAX), and the von Bertalanffy metrics of Linf and K were found within and among latitudes, the greatest variation in some demographic characteristics was found among distance strata across the shelf regardless of latitude. Fish were always relatively smaller at inner shelf reefs and grew more slowly when compared to mid and outer shelf reefs; this was true regardless of the color morph of fish. The oldest fish collected was 11 years old, and there was no consistent variation in age-max among distances from shore. On outer reefs, there was a negative linear relationship with age-max and latitude. This "tropical gradient" of age only explained 34% of the variation; furthermore, this was not found when only the oldest group of fish was considered (top 10%). Fish only reached an age-max of six years on the southernmost reefs. There was a trend for a smaller Linf with latitude but it was not significant and Linf did not vary predictably with water temperature. The sampling ofmarine protected areas (MPAs) and fished zones did not confound the resultant patterns in that fish were not consistently larger or older inMPAs or fished zones. Instantaneous mortality rates were 0.245-0.685; they were highest at inner reefs and also showed no consistentMPA-related patterns. Our study suggested that the mid and outer shelf waters of the GBR appeared best suited for growth of A. polyacanthus. In conclusion, the position on continental shelves dominated other geographical patterns and needs to be considered in spatial models of growth. We suggest that local environmental conditions such as turbidity and the quality and quantity of plankton likely have a strong influence on distance across the shelf-based demographic patterns of planktivores

Protocol for a randomised control trial of methylnaltrexone for the treatment of opioid-induced constipation and gastrointestinal stasis in intensive care patients (MOTION)

Gastrointestinal dysmotility and constipation are common problems in intensive care patients. The majority of critical care patients are sedated with opioids to facilitate tolerance of endotracheal tubes and mechanical ventilation, which inhibit gastrointestinal motility and lead to adverse outcomes. Methylnaltrexone is a peripheral opioid antagonist that does not cross the blood-brain barrier and can reverse the peripheral side effects of opioids without affecting the desired central properties. This trial will investigate whether methylnaltrexone can reverse opioid-induced constipation and gastrointestinal dysmotility.This is a single-centre, multisite, double-blind, randomised, placebo-controlled trial. 84 patients will be recruited from 4 intensive care units (ICUs) within Imperial College Healthcare NHS Trust. Patients will receive intravenous methylnaltrexone or placebo on a daily basis if they are receiving opioid infusion to facilitate mechanical ventilation and have not opened their bowels for 48 hours. All patients will receive standard laxatives as per the clinical ICU bowel protocol prior to randomisation. The primary outcome of the trial will be time to significant rescue-free laxation following randomisation. Secondary outcomes will include tolerance of enteral feed, gastric residual volumes, incidence of pneumonia, blood stream and Clostridium difficile infection, and any reversal of central opioid effects.The trial protocol, the patient/legal representative information sheets and consent forms have been reviewed and approved by the Harrow Research Ethics Committee (REC Reference 14/LO/2004). An independent Trial Steering Committee and Data Monitoring Committee are in place, with patient representation. On completion, the trial results will be published in peer-reviewed journals and presented at national and international scientific meetings.2014-004687-37; Pre-results

Operation of Site Running StratusLab toolkit v1.0

This document reports on one of the significant project milestones concerning the operation of a cloud site running StratusLab's cloud distribution (or toolkit). It is currently running v0.3 of the StratusLab distribution-a beta release of the upcoming 1.0 production release. In particular it presents the WP5 reference cloud service, the evolution of the service up to Month 10 of the project, and the way this service has been exploited to date. A major milestone regarding the exploitation has been the deployment of the first virtualized production grid site running on the project's reference cloud service