Evaluation of different native Streptomyces spp. for effective management of rhizome rot of turmeric

The efficacy of talc based bioformulations containing various biocontrol agents against rhizome rot disease caused by Pythium aphanidermatum in turmeric plants was evaluated under field condition. Indigenous biocontrol agents such as Streptomyceslydicus, Streptomyces griseus and Streptomyces sannanensis belonging to actinomycetes group, Pseudomonas fluorescens (bacterial) and Trichoderma atroviride (fungal) were selected for the biological control of rhizome rot of turmeric. The results indicated a significantly stronger reduction in disease severity in trial plots treated with Bacillus subtilis based commercial fungicide ‘Companion’ when compared to plants treated with indigenous biocontrol agents. However, it was reverse in trial plots in terms of turmeric rhizome yield potential, yield attributes, physiological components, biochemical constituents and quality characteristics of rhizomes. Among 17 treatments, a dual mixture of S. griseus and T. atroviride achieved the best disease control as well as plant growth improvement when compared to single and triple combinations of biocontrol agents. The present study confirms that exploration of microbial formulations containing Streptomyces spp.as soil inoculant to turmeric plants exhibited some benefits to turmeric plant growth as well as controlling rhizome rot disease, which ultimately enhance the overall quality characteristics of rhizomes. Further, our results suggest that a dual combination of biocontrol agents represent a promising method for effective management of rhizome rot of turmeric

Patient’s Perception on Services of Indian Multispecialty Hospitals – A Tqm Approach

No Abstract

Green Synthesis of Piperine/Triton X-100/Silver Nanoconjugates: Antimicrobial Activity and Cytotoxicity

Silver nanoparticles (AgNPs) were synthesized through a green chemical approach using the piperine isolated from black pepper. The physicochemical properties of Triton X-100 coated silver nanoparticles (Triton X-100/Ag NPs) were well characterized by ultraviolet-visible absorption spectroscopy (UV-Vis), powder X-ray diffraction (PXRD), transmission electron microscopy (TEM), selected area electron diffraction (SAED) and energy dispersive X-ray spectroscopy (EDS). The TEM images confirmed the spherical shape of silver nanoparticles. The powder X-ray diffraction analysis revealed the silver nanoparticles exhibiting face-centered cubic (fcc) crystal structure with an average crystallite size of 15 nm. The cytotoxicity effects on HepG2 cells were also evaluated with a series of 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT) assay. The effective toxic concentration of Triton X-100/piperine with silver nanoparticles was too low to damage HepG2 cells. The antibacterial activity results showed that the Triton X-100/piperine/AgNPs efficiently inhibited the two bacteria namely S. aureus and E. coli. The Triton X-100/piperine/AgNPs nanoconjugates were effective in inhibiting the growth of both gram-positive and gram-negative bacteria S. aureus and E. coli

Green Synthesis of Piperine Loaded Gold/Triton X-100 Nanoconjugates: In-vitro Evaluation of Biocompatibility and Anti-Oxidant Activity

In this research, gold nanoparticles (Au NPs) were synthesized by a green chemical approach using the organic natural product piperine isolated from the black pepper. The piperine-Au NPs attached on the Triton X-100 and they were characterized by ultraviolet-visible absorption spectroscopy (UV-Vis), dynamic light scattering spectroscopy (DLS), X-ray powder diffraction (XRD), transmission electron microscopy (TEM), and energy dispersive X-ray spectroscopy (EDS). The TEM analysis confirmed the spherical shape of Au NPs with a diameter of 17-30 nm. The XRD analysis revealed that the Au NPs exhibited fcc crystal structure with an average crystallite size of 26 nm. The biocompatibility of the piperine-AuNPs-Triton X-100 nanoconjugates were examined on Hep-G2 cells. The piperine-AuNPs-Triton X-100 nanoconjugates exhibited better anti-oxidant activity