DNA and biochemical analysis of a potential opaque2 maize population

Maize has low nutritional value because it is poor in essential amino acids lysine and tryptophan, but different mutations have been identified that increase their content. Two high lysine/tryptophan populations from Maize Research Institute genebank (IP1 and IP2) were identified in a previous research. In both populations, analysis with umc1066 opaque2 specific marker detected a recessive (o2), a dominant (O2) and an unknown allele (UA). However, IP2 lacked homozygous recessive o2o2 genotypes. The aim of the present research was to determine by DNA and biochemical analysis if UA allele was a recessive allele and/or if high tryptophan content was due to the o2 or some other mutation. Tree more opaque accessions with different mutations - IP3o5, IP4o14 and IP5floury (no data on type of mutation) were used in biochemical analysis for comparison with IP1 and IP2. Kernels were divided into two samples - with hard and with soft kernels. The UA allele sequencing revealed that it was a dominant allele with four GCCAGA repeats. SSR analysis showed presence of o2 in IP1 in both hard and soft kernels. Decrease in 22 kDa, 19 kDa and 27 kDa zeins in soft kernels was observed only in IP1 and IP2. Tryptophan content was high in soft kernels of IP1 (0.081) and IP2 (0.085), and in both hard and soft kernels of -IP3o5 (0.083 and 0.085, respectively). It can be concluded that IP1 is an o2 mutant and that IP2 carries a high tryptophan mutation other than o2, o5, o14 or floury

Strukovna reforma zabija gol Povijesti?

U članku se opširno prikazuje najavljena strukovna reforma srednjoškolskog obrazovanja u Hrvatskoj. Reforma se planira i provodi bez dovoljno transparentnosti što dovodi do zabrinutosti stručnjaka i praktičara. Autorica prikazuje detalje planirane reforme od pojašnjenja obrazovnih sektora, strukture kvalifikacija na više razina te utjecaj na položaj općeobrazovnih premeta. Školski predmet Povijest prema najavama smanjuje se na samo jednu godinu poučavanja bez obzira na vrstu srednje škole

CBRN crime scene management and investigation

Investigation of a CBRN incident has to be conducted in a systematic, objective, and timely manner in order to collect, preserve and analyse physical evidence from the associated site to be admissible in a criminal or civil proceeding, as well as in prosecution of acts of terrorism and war crimes. The inherently hazardous character of a crime scene involving CBRN agents adds complexity to investigative actions and requires additional expertise, equipment and conditions. The specific investigation strategies are necessary, together with the adapted CBRN detection equipment and forensics methods and procedures. This paper deals with the management of a CBRN crime scene and the specifics of investigation process, including interfaces between CBRN safety and security, and emergency response. The necessary framework, the conduct of operations, and an integrated command structure are outlined. The role of the technical support organisations with adequate CBRN expertises is emphasised

Ex post analysis of road projects: resilience to crisis

This investigation aimed to reveal a mechanism of how different road projects' settings respond to macro-economic crisis. Qualitative and quantitative analyses were performed over a sample of 31 European road projects, in various funding arrangements and life cycle phases, all extracted from the Horizon 2020 BENEFIT project cases database. The project setting is described through a specific combination of project features and/or values of developed indicators. The analysis was applied to identify factors that contributed to projects’ performance regarding the resilience to the global financial crisis of 2007–2008. By doing this, it became possible to determine potential liabilities of projects that are already in their implementation or use phases. The analysis showed there are equally strong contributors to a project’s success within country-specific, as well as project-specific features. In order to boost resilience toward sudden and unpredicted disruptions, several factors have emerged, such as long term planning, investing in top priority projects (preferably medium size investments), with realistic traffic projections and experienced and responsible concessionaires, but also having in place strong regulatory bodies and government support.The identified mechanism of enhancing the resilience to crisis caused by a specific project setting can be beneficial to multiple stakeholders

Novel toxigenic species on maize kernels in Southeastern Europe

In recent years, global climate changes have caused the variability of agro-climatic conditions, which could contribute to the synthesis of higher concentrations of mycotoxins in cereal grains during the growing season and could result in economic losses in the production, as well as in increased risk to human and animal health. These reasons and the fact that new toxigenic species have been identified in Serbia and its neighbouring countries in a few past years, indicate the need for permanent monitoring of mycopopulations on cereals. In Serbia, 30 different species of the genus Aspergillus have been identified, isolated mainly from cereal grains. The uncommonly high frequency and incidence of Aspergillus infestation of maize grain in the last few years were caused by extremely stressful agrometeorological conditions, high temperatures and drought over the period from flowering to waxy maturation of maize. Molecular detection of Aspergillus species collected from different samples of cereal kernels was done by using PCR-RFLP analysis of aflR-aflJ intergenic spacer (IGS). Restriction digestion of PCR products with BglII enzyme gave profiles specific for A. parasiticus - two fragments of 363 and 311 bp, which confirmed the presence of this species in the samples subjected to analysis. Characterization of Fg comlex species was done by DNA sequence-based analysis using primer pairs ef1/ef2. Specific genome fragments were sequenced and analized. Sequences were compared to the data from GeneBank. Most of the tested isolates appeared to represent F. graminearum sensu stricto species, while only two of them were identified as Fusarium boothii and Fusarium vorosii

Ex post analysis of road projects: resilience to crisis

This investigation aimed to reveal a mechanism of how different road projects' settings respond to macro-economic crisis. Qualitative and quantitative analyses were performed over a sample of 31 European road projects, in various funding arrangements and life cycle phases, all extracted from the Horizon 2020 BENEFIT project cases database. The project setting is described through a specific combination of project features and/or values of developed indicators. The analysis was applied to identify factors that contributed to projects' performance regarding the resilience to the global financial crisis of 2007-2008. By doing this, it became possible to determine potential liabilities of projects that are already in their implementation or use phases. The analysis showed there are equally strong contributors to a project's success within country-specific, as well as project-specific features. In order to boost resilience toward sudden and unpredicted disruptions, several factors have emerged, such as long term planning, investing in top priority projects (preferably medium size investments), with realistic traffic projections and experienced and responsible concessionaires, but also having in place strong regulatory bodies and government support. The identified mechanism of enhancing the resilience to crisis caused by a specific project setting can be beneficial to multiple stakeholders

Fly-Through animation at the Faculty of Technical Sciences in Novi Sad

U ovom radu je dat pregled korišćenja Fly Through animacije, posebno u arhitekturi. Specijalno je prikazana primena ove animacije na studijama Računarske grafike-Animacija u inženjerstvu i na studijama Arhitekture i urbanizma, na Fakultetu tehničkih nauka u Novom Sadu. Prikazana su tri istorijski značajna objekta: Gimnazija Jovan Jovanović Zmaj, Departman za Arhitekturu i urbanizam, kao i gradska kuća u Novom Sadu, na čijim modelima su urađene animacije. Postupak izrade ovih animacija je detaljno opisan, redom, kako su objekti i animacije napravljeni.This article describes application of Fly-Through animation, especially in architecture. It shows application of this animation technique on Computer Graphics - Animation in Engineering studies and on Architecture and Urban Planning studies at the Faculty of Technical Sciences in Novi Sad. Three historically significant buildings have been modeled and animated: Gymnasium Jovan Jovanovic Zmaj, Department of Architecture and Urban Planning and a City Hall in Novi Sad. Development process of these models and animations is described in details, step by step