Protein structural class prediction based on an improved statistical strategy

<p>Abstract</p> <p>Background</p> <p>A protein structural class (PSC) belongs to the most basic but important classification in protein structures. The prediction technique of protein structural class has been developing for decades. Two popular indices are the amino-acid-frequency (AAF) based, and amino-acid-arrangement (AAA) with long-term correlation (LTC) – based indices. They were proposed in many works. Both indices have its pros and cons. For example, the AAF index focuses on a statistical analysis, while the AAA-LTC emphasizes the long-term, biological significance. Unfortunately, the datasets used in previous work were not very reliable for a small number of sequences with a high-sequence similarity.</p> <p>Results</p> <p>By modifying a statistical strategy, we proposed a new index method that combines probability and information theory together with a long-term correlation. We also proposed a numerically and biologically reliable dataset included more than 5700 sequences with a low sequence similarity. The results showed that the proposed approach has its high accuracy. Comparing with amino acid composition (AAC) index using a distance method, the accuracy of our approach has a 16–20% improvement for re-substitution test and about 6–11% improvement for cross-validation test. The values were about 23% and 15% for the component coupled method (CCM).</p> <p>Conclusion</p> <p>A new index method, combining probability and information theory together with a long-term correlation was proposed in this paper. The statistical method was improved significantly based on our new index. The cross validation test was conducted, and the result show the proposed method has a great improvement.</p

The influence of protein free calf blood extract eye gel on dry eye after pterygium surgery

AIM: To investigate the influence of protein free calf blood extract eye gel on dry eye after pterygium surgery. <p>METHODS: Thirty six patients(40 eyes)with primary nasal pterygium were enrolled in this study, which were divided into study group and control group randomly, with 20 eyes in each group. All patients received pterygium excision and limbal stem cell autograft surgery and tobramicin dexamethasone eye drops after surgery. Patients of the study group received protein free calf blood extract eye gel while those of the control group received 0.1% sodium hyaluronate eye drops furthermore. Ocular surface disease index(OSDI)questionnaire, tear film break-up time(BUT)and Schirmer's Ⅰ test Ⅰ(SⅠt)were carried before and 3 months after surgery to evaluate the dry eye degree of the patients. <p>RESULTS: There was no statistical difference between the age, gender and size of the pterygium of the study and control groups preoperatively. There was no statistical difference between the OSDI(2.33±1.02 <i>vs</i> 2.32±0.93), BUT(8.80±2.48 <i>vs</i> 8.35±2.28)seconds and SⅠt(4.30±2.30 <i>vs</i> 4.40±2.44)of the two groups preoperatively. There was statistical difference between the OSDI(1.45±0.47 <i>vs</i> 1.81±0.60), BUT(11.20±2.07 <i>vs</i> 9.50±2.40)seconds and SⅠt(8.35±3.13 <i>vs</i> 6.35±2.18)of the two groups 3 months postoperatively, which was also different from that of the preoperative data correspondingly. <p>CONCLUSION: Protein free calf blood extract eye gel could reduce the dry eye after pterygium surgery

Your blush gives you away: detecting hidden mental states with remote photoplethysmography and thermal imaging

Multimodal emotion recognition techniques are increasingly essential for assessing mental states. Image-based methods, however, tend to focus predominantly on overt visual cues and often overlook subtler mental state changes. Psychophysiological research has demonstrated that HR and skin temperature are effective in detecting ANS activities, thereby revealing these subtle changes. However, traditional HR tools are generally more costly and less portable, while skin temperature analysis usually necessitates extensive manual processing. Advances in remote-PPG and automatic thermal ROI detection algorithms have been developed to address these issues, yet their accuracy in practical applications remains limited. This study aims to bridge this gap by integrating r-PPG with thermal imaging to enhance prediction performance. Ninety participants completed a 20-minute questionnaire to induce cognitive stress, followed by watching a film aimed at eliciting moral elevation. The results demonstrate that the combination of r-PPG and thermal imaging effectively detects emotional shifts. Using r-PPG alone, the prediction accuracy was 77% for cognitive stress and 61% for moral elevation, as determined by SVM. Thermal imaging alone achieved 79% accuracy for cognitive stress and 78% for moral elevation, utilizing a RF algorithm. An early fusion strategy of these modalities significantly improved accuracies, achieving 87% for cognitive stress and 83% for moral elevation using RF. Further analysis, which utilized statistical metrics and explainable machine learning methods including SHAP, highlighted key features and clarified the relationship between cardiac responses and facial temperature variations. Notably, it was observed that cardiovascular features derived from r-PPG models had a more pronounced influence in data fusion, despite thermal imaging's higher predictive accuracy in unimodal analysis.Comment: 28 pages, 6 figure

Effects of acute ammonia exposure and post-exposure recovery on nonspecific immunity in Clam Cyclina sinensis

This study aimed to assess the toxicity of ammonia on clam Cyclina sinensis and the post-exposure recovery. With increased exposure to TAN, the alkaline phosphatase (AKP) activities after exposure showed a trend of growing initially and subsequently decreasing, whereas the AKP activities after post-exposure recovery showed an increasing trend. The AKP activities after post-exposure recovery were significantly higher than those in control. The acid phosphatase (ACP) activities in T1 and T2 after post-exposure recovery were higher than those in the control, whereas the ACP activities in T3, T4, and T5 after post-exposure recovery were significantly higher than those in the control. The lysozyme (LZM) activities in T1 and T2 after exposure were significantly higher than those in control, whereas the LZM activities in T3, T4, and T5 after exposure were significantly lower than those in the control. Overall, ACP and LZM in the clams exposed to a low level of TAN (≤ 40 mg/L) can recover to the normal levels completely. However, a 48h recovery period scarcely seems adequate to compensate for AKP, ACP, and LZM activities in the clams exposed to a high level of TAN (> 40 mg/L)