Semi-natural reciprocal sowing experiment established in maritime pine to study local adaptation at early stages of establishment.

<p>Experimental sites and population's origin are shown in red triangles. Climodiagrams (mean monthly temperature in red and monthly precipitation in blue) of both sites are also given (data from the Spanish National Meteorological Agency, after corrections using Gonzalo's phitoclimatic model for Spain <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0109132#pone.0109132-GonzaloJimnez1" target="_blank">[44]</a>). The experimental design (in the center of the figure) consisted in a split-plot with four replicates in each microenvironment (open canopy, exemplified in the photo on the left side; and closed canopy, the photo on the right side); dark grey and light grey boxes represent the two origins tested (Coca and Calderona).</p

Kaplan-Meier estimators for early survival in Calderona, for two geographically-distant maritime pine origins.

<p>Four functions are shown, each one corresponding to a combination of origin and microenvironment (i.e. open- or closed-canopy cover, see legend in the figure). Differences across origins-microenvironments were highly significant (<i>p</i><0.001), as shown by log-rank tests (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0109132#pone.0109132.s005" target="_blank">Table S1</a>).</p

Kaplan-Meier estimators for non-emergence (A–B) and early survival (C–D) probability in the reciprocal sowing experiment.

<p>Four functions are shown, each corresponding to a combination of site and microenvironment (i.e. open- or closed-canopy cover, see legend in the figure). Differences across sites-microenvironments were highly significant (<i>p</i><0.001), as shown by log-rank tests (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0109132#pone.0109132.s005" target="_blank">Table S1</a>).</p

Odds ratios (OR) for the discrete-time logistic survival model.

<p>Number of observations generated: 4,560 (2,188 seedlings)</p><p>Calderona <i>site</i>, Calderona <i>origin</i>, closed canopy, and the second-time interval (between census one and two, c. 20–52 days after seedlings emerged) are the reference levels (given also between parentheses for each factor/model). SE: standard error; CI: confidence intervals.</p><p>Odds ratios (OR) for the discrete-time logistic survival model.</p