Study of the Altered Anisotropy of Erythrocyte Ghost Membrane upon Interaction with Phytoreduced Negatively Charged Gold Nanoparticle from Celosia cristata and Vigna radiata

Synthesis of negatively charged gold nanoparticles (GNP) was done from seed extract and seed electrolyte of two locally available plant sources Celosia cristata and  Vigna radiata without using any toxic chemicals and it is a rapid reduction way. Standard techniques of spectroscopy and   advanced  microscopic study was done in characterizing the particles and simultaneously the phytochemicals present in the electrolyte and extract were also spotted using established process. The effects of nanoparticles on live red blood cells were analyzed by calculating hemolysis percentage. Evaluations of GNP activity when interacted with erythrocyte ghost membrane and their impact on membrane fluidity and anisotropy were also studied as negatively charged GNP are less delineated in previous history. The results showed that negatively charged gold nanoparticles were reduced by flavonoids and phenols present in seed extract and electrolyte of plants and they are hemocompatible and can help to fluidize the membrane of erythrocyte ghost cells.The whole experiment suggested a simple, ecofriendly approach of synthesizing negatively charged nanogold which are safe to live cells that can change the fluidity parameter of membrane constituent by decreasing the anisotropy which can further help in curing health problem related with cell membrane issue

Exciplexes of Polycyclic Aromatic Hydrocarbons with Halomethanes

902-90

ELECTRICAL RESISTIVITY AND ACTIVATION ENERGY OF COBALT ACETATE TETRAHYDRATE DOPED MULLITE

Mullite composites have been synthesized at 400°C, 800°C, 1000°C and 1300°C via the sol-gel technique in the presence of cobalt. The electrical resistivity and activation energy of the composites have been measured and their variation with concentration of the metal ion doping has been investigated. The resistivity of doped mullite decreases rapidly from 400°C-800°C more gently from 1000°C-1300°C. The lowering of resistivity is due to the 3d orbital electrons and the concentration of cobalt ions. X-ray analysis confirms the presence of Co2+ ions in mullite, which entered the octahedral site. The Co2+ ion which substituted Al3+ ion in the octahedral site of mullite structure appeared to be efficient in reducing the resistivity. This has been confirmed due to the results of activation energy of resistivity/band gap energy, the Eg which was lowest for concentration 0.15 M. As the concentration increases, these ions lower the resistivity of mullite to a minimum

In situ synthesis of environmentally benign montmorillonite supported composites of Au/Ag nanoparticles and their catalytic activity in the reduction of p-nitrophenol

In the present work, composites of montmorillonite clay supported silver and gold nanoparticles were synthesized by in situ chemical reduction method and characterized by X-ray diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR), UV-vis spectroscopy and Transmission Electron Microscopy (TEM). The clay-nanoparticle composites were synthesized at two different temperatures (25 degrees C and 75 degrees C) where nanoparticle size was found to depend on synthesis temperature. The distribution of the catalytic nanoparticles was uniform in the clay matrix with sizes in the range of 20-45 nm (at 25 degrees C) and 5-15 nm (at 75 degrees C), respectively. Catalytic activity of the clay-nanoparticle composites were monitored by UV-visible spectroscopy using p-nitrophenol and NaBH4 as model reactants. The best catalytic efficiency was observed in the case of silver-clay nanocomposites with a rate constant of 5.6 x 10(-3) s(-1)

Scientific guidelines for preclinical research on potentised preparations manufactured according to current pharmacopoeias-the PrePoP guidelines.

OBJECTIVE Pharmacopoeias regulate the manufacture of potentised pharmaceutical preparations used in different branches of complementary and integrative medicine. The physicochemical properties and biological activity of these preparations are often investigated in preclinical research, yet no guidelines for experimental research currently exist in this area. The present PrePoP guidelines aim to provide recommendations to promote high-quality, statistically sound, and reproducible preclinical research on potentised preparations. METHODS Input was gathered from researchers nominated by the relevant scientific societies using a simplified Delphi consensus approach covering the most relevant aspects of basic research methodology in the field including appropriate controls, sample preparation and handling, and statistics. After three rounds of feedback, a consensus was finally reached on the most important aspects and considerations for conducting high-quality research on potentised preparations. RESULTS We present a series of recommendations on a range of topics including experimental controls, system stability, blinding and randomisation, environmental influences, and procedures for the preparation of potentised samples and controls, and we address some specific challenges of this research field. CONCLUSION This expert consensus process resulted in a robust set of methodological guidelines for research on potentised preparations and provides a valuable framework that will inform and improve the quality of subsequent research in this emerging field. PLEASE CITE THIS ARTICLE AS Tournier AL, Bonamin LV, Buchheim-Schmidt S, Cartwright S, Dombrowsky C, Doesburg P, Holandino C, Kokornaczyk MO, van de Kraats EB, López-Carvallo JA, Nandy P, Mazón-Suástegui JM, Mirzajani F, Poitevin B, Scherr C, Thieves K, Würtenberger S, Baumgartner S. Scientific guidelines for preclinical research on potentised preparations manufactured according to current pharmacopoeias-the PrePoP guidelines. J Integr Med. 2024; Epub ahead of print

Role of planar lipid structure in transmembrane charge conduction

143-154Using planar lipid membrane as the model for biological membranes, we have studied the role of lipid medium in the mechanism of transmembrane charge transport. <span style="font-size:14.0pt; font-family:HiddenHorzOCR;mso-bidi-font-family:HiddenHorzOCR">Here we present a review of our results which indicate that (a) both electrons and ions are charge carriers across these membranes, (b) hydrophilic pores are formed in the planar lipid membrane ", providing the required ionic pathways, (c) membrane I-V characteristics are nonlinear and are monitored by the presence of external agents in the bathing solution, temperature and frequency of the applied electric field. We show that these observations can be explained in the light of semiconducting nature of lipid molecules, double electrode behaviour of the membrane and the theory of charge conduction in binary, inhomogeneous, disordered systems. </span

A review of Basic Research on Homoeopathy from a physicist′s point of view

Homoeopathic medicine has been one of the world′s most widely practiced alternative therapies. However, that the potency of a homoeopathic medicine increases with dilution followed by succussion (together termed as potentization) has thrown challenges to the scientific community at large. A recent hypothesis, advanced by us and others, is that due to the process of potentization, the size of the constituent particles decreases and eventually reaches nanodimension. This decrease in size with increase in potency has been verified by scanning electron microscopy and dynamic light scattering studies. The increase in potency is manifested in its increased effect on membrane fluidity. The change in potency also leaves its signature on Ultraviolet-Visible spectra, Fourier transform infrared radiation spectra and Raman spectra. We have taken one step further to carry this nano-dimensional property of homoeopathic medicine and put it into several technical applications. And in so doing, we have connected the important, old, un-quantifiable effects with the latest quantifiable technology and opened up an era of applications with more possibilities

Photovoltage Generation in Dye-Probed Bilayer Lipid Membranes

1046-104