Additional file 1: of Engineered recombinant protein products of the avian paramyxovirus type-1 nucleocapsid and phosphoprotein genes for serological diagnosis

Cut-off values of indirect ELISAs based on recombinant avian paramyxovirus NP and P gene products. Provides data that elucidate the fixing of a threshold for indirect ELISA (DOCX 12 kb

Stereoselective Ring-Opening Polymerization of <i>rac</i>-Lactide Using Organocatalytic Cyclic Trimeric Phosphazene Base

Phosphazene base is an important organocatalyst in polymer chemistry owing to its high activity and versatility. In this contribution, we demonstrate that cyclic trimeric phosphazene base (<b>CTPB</b>) can catalyze stereoselective ring-opening polymerization (ROP) of <i>rac</i>-lactide (<i>rac</i>-LA) to produce isotactic stereoblock PLA (<i>P</i>_i up to 0.93). The polymerizations are highly controlled, as evidenced by linear relationship between molecular weights (MW) and monomer conversions and the narrow dispersity (<i>Đ</i> = <i>M</i>_w/<i>M</i>_n) of the resulted polymers with high fidelity of end groups. The investigations on polymerization parameters show that the tacticity of produced PLA depends on the polymerization temperatures and solvents, while the kinetic studies reveal a faster rate for ROP of l-LA as compared to <i>rac</i>-LA under same conditions. Based on these results, the chain end control mechanism is proposed to explain the production of isotactic stereoblock PLA from <i>rac</i>-LA by an achiral catalyst

Low Mfn2 expression attenuates the blastocyst formation rate and cleavage speed.

<p>In T group, the cleavage speed was reduced compared with that in control group, the blastocyst formation rate was significantly reduced in the T group compared with the C group. C1 and T1: 4-cell fertilized eggs, C2 and T2: 8-cell fertilized eggs. C3 and T3: Blastocyst. Data are means ± SD of 3 separate experiments.</p

Mfn2 expression in mouse fertilized eggs after transfection.

<p>Mfn2 mRNA expression was detected by qPCR and its level is significant lower in T group than that in C group (A). Mfn2 protein levels were determined by Western blotting (B). The quantitation of Mfn2 protein levels. C1 and T1: 4 cells fertilized eggs, C2 and T2: 8 cells fertilized eggs, C3 and T3: blastocyst. *<i>P</i><0.05. Data are means ± SD of 3 separate experiments (C).</p

Blastocyst formation rate.

<p>The blastocyst formation rate of the fertilized eggs collected from 5-week-old Kunming mice. The table presents the number of fertilized eggs collected from five mice. The values represent the rate of successful blastocyst development.</p><p>* <i>P</i>>0.05 <i>vs</i> control-siRNA transfected,</p><p>** <i>P</i><0.01 <i>vs</i> control-siRNA transfected.</p><p>Blastocyst formation rate.</p

Log-linear relationships between the regional net increase in sum of average <i>N</i> (based on the medium estimate) from rural grid cells and the size of source population at three different administrative levels.

<p>Log-linear relationships between the regional net increase in sum of average <i>N</i> (based on the medium estimate) from rural grid cells and the size of source population at three different administrative levels.</p

Linear relationships between observed changes in location request across rural grid cells during the Chinese New Year period and the corresponding amount of regional out-migrants at three different levels.

<p>Linear relationships between observed changes in location request across rural grid cells during the Chinese New Year period and the corresponding amount of regional out-migrants at three different levels.</p

An estimate of rural exodus in China using location-aware data - Fig 4

<p>(A) Country-level time series of location request in social media uses for different areas. Right inset shows the corresponding number of grid cells. (B) Temporal changes in proportion of location request relative to the total country-level sum of <i>N</i> for different areas as shown in (A) except TS1.</p

Multi-level quantitative comparisons between proportions of regional sum of declined and increased location request accounting for the nationwide sum for urban and rural domains, respectively, demonstrate regional imbalances in rural migrations into urban areas.

<p>The deviation from the balanced diagonal is measured using multiples of statistical error. Here, the upper and the lower deviation line represent and , respectively.</p

Summary of determinative criteria for the estimate of grid cell-level rural depopulation and its lower and upper bound.

<p><i>NR</i>_<i>b</i> and <i>NR</i>_<i>a</i> stand for average <i>N</i> before and after <i>TP</i> for rural grid cells, respectively.</p