A study of the applicability of the electron microprobe to a quantitative analysis of K and Na in single human red blood cells

Conventional analytical procedures only permit the determination of average concentrations of cell constituents in many cells. In contrast, the electron microprobe allows one to measure the amounts of the various elements present in individual cells. However, the application of the microprobe encounters the difficulty that during the preparation of the cells for analysis uncontrolled losses of diffusible cell constituents such as K and Na may take place. In the present paper an attempt was made to design a technique which minimizes such losses. A standardization procedure was applied to assess the success of the preparation procedure. This involved the preparation of cells of varying K and Na contents and a comparison of the averages of the K and Na X-ray intensities as measured in many single cells by means of the electron microprobe with the average K and Na contents of the population as determined by flame photometry

QUANTITATIVE ELECTRON PROBE ANALYSIS OF HAEMOLYMPH AND URINE COMPOSITION FROM INSECT CALLIPHORA ERYTHROCEPHALA DURING METAMORPHOSIS

Les teneurs en Na, K, Mg, Ca et P de l'urine distale et de l'hémolymphe de la mouche Calliphora e. ont été mesurées au cours de la métamorphose à l'aide d'un analyseur à sonde électronique.Na, K, Mg, Ca and P concentrations in distal urine and haemolymph of the blowfly Calliphora e. have been measured during metamorphosis. Quantitative analysis of liquid microsamples has been performed with an electron probe analyser

ELECTRON PROBE ANALYSIS OF SAP EXUDATES OF TWO VARIETIES OF WHEAT (TRITICUM AESTIVUM L.)

La composition de la sève du blé a été déterminée par analyse à la microsonde électronique. Cette technique a permis d'analyser quantitativement les éléments K, Si, P, Cl, S Mg, Ca et Na contenus dans les exsudats.The sap composition was determined by electron probe analysis. With this technics, it has been possible to make the quantitative analysis of the following elements : K, Si, P, Cl, S, Mg, Ca and Na contained in the exudates