Prevalence Atopobium vagina in vaginal samples of symptomatic non-pregnant women

Introduction: Bacterial vaginosis is not a mono-factorial infection. A synergism of microaerophilic bacteria, Mycoplasma spp., and anaerobic bacteria such as Atopobium vaginae, Porphyromonas spp., Bacteroides spp., Prevotella spp., and others are involved in these infections. The aim of present study was to determine the prevalence of Atopobium vaginae in non-pregnant women suffering from bacterial vaginosis. Materials and Methods: A total of 102 non-pregnant women who referred to ShahidAkbarabadi hospital in Tehran were tested for bacterial vaginosis. In order to isolate Atopobiumvaginae the sample was cultured on Colombia agar containing Amphotericin B, Nalidixic acid and Colistin. Additionally, they were simultaneously cultured on blood agar plates containing fresh human blood and Amphotericin B under anaerobic conditions. After extraction DNA from colonies and vaginal specimens, PCR amplification was performed by using specific primers for detection of Atopobiumvaginae. Results: From a total of 102 women who referred to the hospital, 38 cases were confirmed for bacterial vaginosi. With PCR assay, 25 of these 38 cases (65) were positive for Atopobiumvaginae. Conclusion: This is the first report of isolation of Atopobiumvaginae in Iran. The results of this investigation points to a clear association of Atopobiumvaginae with bacterial vaginosis and Atopobiumvaginae should also be considered as a probable etiological agent. © 2016, Semnan University of Medical Sciences. All rights reserved

Isolation of Atopobium vaginae in vaginal and urine samples of Iranian women, the first report

Bacterial vaginosis is not a mono-factorial infection. A synergism of microaerophilic bacteria, Mycoplasma spp., and anaerobic bacteria such as Atopobium vaginae, Porphyromonas spp., Bacteroides spp., Prevotella spp., and others are involved in these infections. The aim of present study was to determine the prevalence of Atopobium vaginae in non-pregnant women suffering from bacterial vaginosis.A total of 102 non-pregnant women who referred to Shahid Akbarabadi hospital in Tehran were tested for bacterial vaginosis. Bacterial culture was performed on Columbia agar containing 2mg/ml Amphotericin B, 30mg/ml Nalidixic acid and 4mg/ml Colistin. Additionally, they were simultaneously cultured on blood agar plates containing fresh human blood and Amphotericin B under anaerobic conditions. Finally, PCR using Atopobiumvaginae specific primers were also carried out on extracted DNA from grown colonies as well as from vaginal specimens. From a total of 102 symptomatic women who referred to the hospital, 37 (38/102) were classified as bacterial vaginosis, 43 (44/102) were classified as intermediate status and 20 (20/102) women were asymptomatic. Sixty urine samples were collected. Atopobium vaginae were detected in 66 (25/38) of bacterial vaginosis cases and in 10 (4/44) samples with intermediate vaginal flora. It was not detected in asymptomatic women. The bacterium was seen in 10 (3/30) women with urinary tract infection and none in pregnant women.This is the first report of Atopobiumvaginae isolation in Iran. The results of this investigation point to a clear association ofAtopobiumvaginaewith bacterial vaginosis. It should therefore be considered as a probable etiological agent

Effect of moisture content on textural attributes of dried figs

Due to their soft texture consumers prefer moist figs, which has motivated fig processors to increase the production of this product. However, as water enhances the browning reaction rate, moisture content optimisation of moist figs is very im- portant. Processed figs must have suitable texture softness with browning kept to a minimum. The purpose of this study was to exa- mine the effect of moisture content on the textural attributes of dried figs. Hardness, compression energy, gradient, gumminess and chewiness of fig samples decreased with moisture content exponentially, whereas the trend of springiness and cohesiveness with change of moisture content was nearly constant. Moreover, in the texture profile analysis plot of rehydrated figs, the presence of negative area is an indication of adhesiveness which was zero in control dried figs. The results of the texture profile analysis tests proved the existence of a critical moisture content of about 18.4%, above which no significant effect of moisture content on textural parame- ters was found. The glass-rubber transition results from differential scanning calorimeter may explain the different texture profile analysis attributes of dried figs compared with rehydrated figs