Phytochemical screening and antioxidant activity of Diospyros lotus L. fruits grown in Turkey

The present study aimed to analyze total ascorbic acid content (TAC), total phenolic content (TPC), total flavonoid content (TFC), phenolic composition, fatty acid profiles, and antioxidant activity of Diospyros lotus L. fruits grown in Turkey. The TAC, TPC and TFC of D. lotus extracts were 13.9, 130.3, and 12.7 mg/100 g, respectively. Phenolic compounds, gallic acid, vanillic acid, caffeic acid, p-coumaric acid, trans-ferulic acid, sinapic acid, naringin, rutin trihydrate, resveratrol, ellagic acid, trans-cinnamic acid, and quercetin were identified in persimmon samples. The 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) – ABTS and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activities and ferric reducing antioxidant power (FRAP) of fruit extracts were found to be 556.3 µmol/g, 69.6%, and 52.4%, respectively. Fructose was identified as the major sugar (371.01 mg/g), while sucrose was not detected. A total of 17 different fatty acids were identified. Most abundant saturated fatty acid (SFA), monounsaturated fatty acid (MUFA), and polyunsaturated fatty acids (PUFA) were palmitic acid (19.66%), palmitoleic acid (13.28%), and linoleic (18.04%) and gamma-linolenic (11.66%) acids, respectively

Determination of fatty acids and volatile compounds in fruits of rosehip (Rosa l.) species by HS-SPME/GC-MS and IM-SPME/GC-MS techniques

In this study, we aimed to compare fatty acid and volatile compound compositions of four rosehip species, namely Rosa pimpinellifolia, R. villosa, R. canina, and R. dumalis, by gas chromatography with flame ionization detector (GC/FID) and headspace and immersion solid-phase microextraction gas chromatography-mass spectrometry (HS-SPME/GC-MS and Im-SPME/GC-MS) techniques. The total lipid contents in fruits of the rosehip species varied from 5.83% (R. villosa) to 7.84% (R. dumalis). A total of 21 fatty acids were detected and quantified. In all species, except R. canina, polyunsaturated fatty acids (PUFAs) predominated over saturated fatty acids (SFAs) and monounsaturated fatty acids (MUFAs). Palmitic acid is the major SFA in R. villosa (5.50%), R. canina (8.27%), and R. dumalis (7.46%). Oleic acid is the most abundant MUFA, and linoleic and ?-linolenic acids are the most abundant PUFAs. Sixtytwo volatile compounds were detected by the HS-SPME/GC-MS technique, and 54 volatile compounds were determined by the Im- SPME/GC-MS technique. Fifty-three volatile components of rosehips have been detected for the first time in this study. While 19 acids, 9 aldehydes, 6 ketones, 18 alcohols, 5 esters, 2 terpenes, and 2 phenols were identified by HS-SPME/GC-MS, 20 acids, 5 aldehydes, 8 ketones, 13 alcohols, 5 esters, 1 terpene, and 2 phenols were identified by Im-SPME/GC-MS. The HS-SPME/GC-MS technique allowed identification of a larger number of volatile compounds and thus is more efficient than the Im-SPME/GC-MS technique. © TÜBİTAK

S_allele identification and genetic diversity analysis of apricot cultivars

In this study, the sexual incompatibility and S-allele diversity of 24 Turkish apricot cultivars, Paviot and Sakıt-1 as parents and 127 F1 progenies were identified by polymerase chain reaction (PCR) and sequencing techniques. Additionally, genetic diversity and relatedness among the 24 cultivars were determined using 18 simple sequence repeat (SSR) markers from the genus Prunus. PCR for S_alleles identified nine different S-RNase alleles in the 24 apricot cultivars, namely Sc, S1, S2, S8, S9, S20, S24, S52, and S53. All primers amplified only one S_allele in the cultivars Adilcevaz-1, Adilcevaz-3, Ethembey, Pasamismisi, Canakkale, and Soganci. Most of the Turkish cultivars were self-incompatible. The Sc allele was present in only three cultivars (Canakkale, Ethembey, Imrahor) that are, therefore, self-compatible. The S_alleles of cultivars Paviot and Sakıt-1 displayed homology with the Sc, S2 and S20, and S52 alleles. In the 127 F1 genotypes, the two S_alleles of Paviot were inherited by roughly half of the offspring, while about 76% of the offspring inherited the S52 allele from Sakıt-1, and less than 24% inherited S20. The amplification using all SSR 18 primers was successful and produced 128 polymorphic alleles with an average of 7.11 alleles per locus. Among the apricot cultivars studied, expected heterozygosity (He) ranged from 0.33 to 0.72, observed heterozygosity (Ho) ranged from 0.42 to 1.00, PIC values were between 0.28 and 0.89, and similarity rates were between 0.30 and 0.68. The cultivars Levent and Ozal were genetically closest (0.68) while cultivars Sakıt-3 and Sogancı were the most distinct (0.30). © 2017 The Journal of Horticultural Science & Biotechnology Trust.113Z540This work was funded by The Scientific and Technological Research Council of Turkey (TUBITAK) [grant number: 113Z540]

Characterization of bioactive compounds in rosehip species from East Anatolia region of Turkey

The objective of this work was to determine some bioactive compounds for four different rosehip species (Rosa L.), growing in the East Anatolia region of Turkey. It was determined that the average fruit weights of the species varied between 9.8 g (R. dumalis) and 34.5 g (R. canina). The total soluble solids showed statistically significant variations among the rosehip species (14-22 °Brix). The acidity was inversely proportional to total soluble solids and ranged between 1.00% (R. canina) and 2.67% (R. villosa). The highest total phenolic, L- ascorbic acid contents and the highest total antioxidant capacity were found in R. canina. The total phenolic, total anthocyanin, total dry matter, and L-ascorbic acid contents and the total antioxidant capacity of the rosehip species ranged as follows 1081-6298 mg gallic acid equivalent/100 g, 2.43-3.72 mg/100 g, 40.1-56.7%, 24.93-754.48 mg/100 g, and 10.04-97.95 mmol trolox equivalent/g, respectively. Glucose was the most common sugar in Rosa species (5.99-12.48 g/100 g), the major organic acid in the rosehip species was citric acid (0.48-1.05 g/100 g). A dendogram based on some pomological and biochemical characteristics of the rosehip species were grouped into 2 main clusters. Findings on the biochemical characteristics of the species will provide insights to plant breeders/growers and for further research. © 2016, Chiriotti Editori. All rights reserved