1 research outputs found
Quantitative Proteomics Reveals That Peroxidases Play Key Roles in Post-flooding Recovery in Soybean Roots
Soybean is an important legume crop
that exhibits markedly reduced growth and yields under flooding conditions.
To unravel the mechanisms involved in recovery after flooding in soybean
root, gel-free proteomic analysis was performed. Morphological analysis
revealed that growth suppression was more severe with increased flooding
duration. Out of a total of 1645 and 1707 identified proteins, 73
and 21 proteins were changed significantly during the recovery stage
following 2 and 4 days flooding, respectively. Based on the proteomic,
clustering, and in silico protein–protein interaction analyses,
six key enzymes were analyzed at the mRNA level. Lipoxygenase 1, which
was increased at the protein level during the recovery period, was
steadily down-regulated at the mRNA level. The peroxidase superfamily
protein continuously increased in abundance during the course of recovery
and was up-regulated at the mRNA level. HAD acid phosphatase was decreased
at the protein level and down-regulated at the transcript level, while
isoflavone reductase and an unknown protein were increased at both
the protein and mRNA levels. Consistent with these findings, the enzymatic
activity of peroxidase was decreased under flooding stress but increased
significantly during the recovery sage. These results suggest that
peroxidases might play key roles in post-flooding recovery in soybean
roots through the scavenging of toxic radicals