いんげんまめ(Phaseolus vulgaris L)の糖質分解酵素:ガラクタナーゼの精製および性質

Galactanase activity in a homogenized preparation of the kidney beans, Phaseolusvulgaris L was examined together with the other carbohydrase activities. This enzymewas purified by the affinity column and gel-filtration column chromatography. SDSPAGEshowed the enzyme had molecular weight of 34kDa. The enzyme hydrolyzed the substrate galactan by endo-type of action and produced several galacto-oligosaccharides.Because the substrate galactan used in this experiment was isolated from the gum arabic,which contained β-1, 3 - galactan as a major component, this enzyme wouldcorrespond to β- 1, 3 - galactanase

多糖ゲルによる糖質加水分解酵素のアフィニティ-クロマトグラフィ-

Soluble starch, pectic acid and alginic acid were cross-linked by polyacrylamide. Several enzymes, including commercially available specimen and food material sources, were examined for their specificities to the polysaccharide gels. α-Amylase showed high affinity for the starch-gel in the presence of 3M ammonium sulfate and eluted with the buffer solution containing no ammonium sulfate. Pectinase and alginate-lyase bound to the pectin-gel and the alginate-gel, respectively. On the other hand, galactanase form the common bean (Phaseolus vulgaris L) bound to the starch-gel ; however, trehalase did not bound to this gel. Highly purified alginate-lyase was obtained by the current affinity chromatography method. Based on these results, relationship between the chemical structure of the polysaccharide-gels and the substrate specificity of the enzymes was discussed

エリンギ(Pleurotus eryngii)の子実態における糖質および糖質分解酵素の特徴

Carbohydrate components and their related carbohydrate activities in a homogenized preparation of the fruit body of king oyster mushroom (eryngii), Pleurotus eryngii, were studied in detail. The low-molecular weight fractions contained a significant amount of trehalose compared with other constituents of mono-saccharides, and its concentration was 18.7% of the dry weight. Polysaccharides in the high-molecular weight fraction were separated into four fractions, A~D, which were analyzed for the yields and sugar components. The polysaccharide fractions were also analyzed by the ion-exchange and gel-filtration column chromatography, and enzymatic hydrolysis. The results suggested that typical polysaccharide components in the fruit body of eryngii might be β-glucan and galactan, corresponding to the known results obtained by the liquid-cultured eryngii. A crude enzyme solution was prepared from the fruit body of eryngii, and various carbohydrase activities were measured using 27 substrates at pH 5.2, 6.5 and 7.8. A higher enzyme activity was detected for the substrates of laminarin and trehalose. Occurrence of trehalase activity correlated with the high concentration of trehalose in the fruit body of eringii

エリンギ(Pleurotus eryngii)のトレハラーゼ : 酵素の分布、精製および性質について

Trehalase activity in a homogenized preparation from the fruit body of king oyster mushroom (eryngii), Pleurotus eryngii was studied in detail. Distribution of trehalose and trehalase activity was higher in the cap (pileus) fraction than in the other positions such as stalk (stipe). The tretalase peak 1 was purified by the Sephacryl S-200 and Toyopearl HW-55 column chromatography. Electorophoretic analysis of trehalase gave values of molecular weight of 36~37kDa, and oligomeric forms and blycoprotein forms of trehalase were suggested by the band patterns of resulting gels. Optimum conditons of pH and temperature were determined to be pH5.0 and 30℃, respectively. Trehalase peak 1 showed higher reactivity to α-, α-trehalose, whereas peak 2 enzyme showed higher activity to β-,β-trehalose, methyl-β-glucoside, and cellobiose, indicating that this enzyme might have a substrate specificity such asβ-glucosidase

RUNX1-ETO (RUNX1-RUNX1T1) induces myeloid leukemia in mice in an age-dependent manner

10.1038/s41375-021-01268-4Leukemia35102983-298