Production of L(+)-Lactic Acid from Mixed Acid and Alkali Hydrolysate of Brown Seaweed

The species of brown seaweeds, Laminaria japonica is commercially cultivated in Japan. Mannitol and uronic acid were the main component of mono sugar produced from the saccharification of L. japonica which hydrolysed with H2SO4 or NH4OH. The mannitol concentration of L. japonica (5w/v%) hydrolysate using 0.5v/v% H2SO4 or 1v/v% NH4OH were 15.84g/L and 13.87g/L, respectively. Hydrolysates from both acid and alkali hydrolysis were mixed together for neutralization as well as to obtain higher mannitol concentration of 15.18g/L. Among the mono sugar in the hydrolysate, Mannitol was the main substrate for the lactic acid fermentation by Lactobacillus rhamnosus. L(+)-Lactic acid with 97.9% of optical purity was successfully produced at the yield of 14.42g/L (Yp/s = 94.99%)

Potential Use of Gelidium amansii Acid Hydrolysate for Lactic Acid Production by Lactobacillus rhamnosus

Galactose and glucose are the main monosaccharides produced from the saccharification of Gelidium amansii. They were hydrolysed with 3 % (by volume) H2SO4 at 140 °C for 5 min and obtained at concentrations of 19.60 and 10.21 g/L, respectively. G. amansii hydrolysate (5 %, by mass per volume) was used as a substrate for L(+)-lactic acid production by Lactobacillus rhamnosus. The maximum lactic acid yield (YP/S) was 42.03 % with optical purity of 84.54 %. Lactic acid produced from G. amansii hydrolysate can be applicable, among others, for the production of lactic acid esters, like ethyl or methyl lactate, and disinfectant in seaweed cultivation

Production of mono sugar from acid hydrolysis of seaweed

The purpose of this work was to optimize the process conditions for the saccharification of macroalgae (seaweed) into mono sugar using the following parameters such as: Amount of biomass, catalyst concentration, temperature and reaction time. The major component of Ulva pertusa (green seaweed), Laminaria japonica (brown seaweed) and Gelidium amansii (red seaweed) is carbohydrate which can be converted into variety of sugars using sulfuric acid with hot-compress treatment. Rhamnose (37.89 wt %) and glucose (16.14 wt %) were extracted from dried U. pertusa, while galactose (49.32 wt %) and glucose (12.62 wt %) were extracted from dried G. amansii. Mannitol (31.53 wt %) was produced from dried L. japonica

Primjena kiselinskog hidrolizata alge Gelidium amansii u proizvodnji mliječne kiseline s pomoću bakterije Lactobacillus rhamnosus

Galactose and glucose are the main monosaccharides produced from the saccharification of Gelidium amansii. They were hydrolysed with 3 % (by volume) H2SO4 at 140 °C for 5 min and obtained at concentrations of 19.60 and 10.21 g/L, respectively. G. amansii hydrolysate (5 %, by mass per volume) was used as a substrate for L(+)-lactic acid production by Lactobacillus rhamnosus. The maximum lactic acid yield (YP/S) was 42.03 % with optical purity of 84.54 %. Lactic acid produced from G. amansii hydrolysate can be applicable, among others, for the production of lactic acid esters, like ethyl or methyl lactate, and disinfectant in seaweed cultivation.Galaktoza i glukoza su glavni monosaharidi koji nastaju saharifikacijom alge Gelidium amansii. Kiselinskom hidrolizom algi pomoću 3 %-tne otopine H2SO4 pri 140 °C tijekom 5 min dobiveno je 19,60 g/L galaktoze i 10,21 g/L glukoze. Hidrolizat alge Gelidium amansii (5 % m/V) upotrijebljen je kao podloga za proizvodnju L(+)-mliječne kiseline s pomoću bakterije Lactobacillus rhamnosus. Najveći prinos (YP/S) mliječne kiseline, optičke čistoće od 84,54 %, bio je 42,03 %. Mliječna kiselina dobivena iz hidrolizata alge Gelidium amansii može se primijeniti za proizvodnju estera mliječne kiseline, kao što su etilni ili metilni laktat, ili kao dezinficijens pri uzgoju morskih trava

Potential Use of Gelidium amansii Acid Hydrolysate for Lactic Acid Production by Lactobacillus rhamnosus

Galactose and glucose are the main monosaccharides produced from the saccharification of Gelidium amansii. They were hydrolysed with 3 % (by volume) H2SO4 at 140 °C for 5 min and obtained at concentrations of 19.60 and 10.21 g/L, respectively. G. amansii hydrolysate (5 %, by mass per volume) was used as a substrate for L(+)-lactic acid production by Lactobacillus rhamnosus. The maximum lactic acid yield (YP/S) was 42.03 % with optical purity of 84.54 %. Lactic acid produced from G. amansii hydrolysate can be applicable, among others, for the production of lactic acid esters, like ethyl or methyl lactate, and disinfectant in seaweed cultivation