In silicoCharacterization of UGT74G1Protein in Stevia rebaudianaBertoni Accession MS007

Due to its low-calorie property, Stevia rebaudianais being promoted as an alterna-tive sweetener for diabetic and obese patients. The steady demand in the market for high-quality stevia extracts presents a challenge for the enhanced production of ste-viol glycosides that are safe for human consumption. This study characterized the structure and content of the gene involved in the production of UGT74G1protein for Steviarebaudianaaccession MS007 through in silicoanalysis using a transcrip-tome dataset of stevia MS007. Homologous search using BLASTp shows high sim-ilarity to Q6VAA6 RecName: Full=UDP-glycosyltransferase 74G1 (S. rebaudiana) as the top hit sequence. InterPro family and domain protein motif search revealed UDP-glucuronosyl/UDP-glucosyltransferase (IPR002213) and UDP-glycosyltrans-ferase family, conserved site (IPR035595). The phylogenetic tree construction was done by selecting 14 out of 102 protein sequences from BLASTp search. The phy-logenetic analysis revealed a high value of bootstrapping, which was 100, indicating the high similarity betweenUGT74G1 (Q6VAA6.1 and Cluster-31069.45201) in S. rebaudiana. ProtParam ExPASy, PSIPRED,and Phyre2 computed the primary, sec-ondary, and tertiary structures for UGT74G1protein. The UGT74G1predicted ter-tiary structure scored 100.0% confidence by the single highest scoring template and coverage of 96%. The model has dimensions (Å) of X: 57.609, Y: 70.386, and Z: 58.351. Outcomes of this research will help enhance understandingUDP-glycosyl-transferase 74G1 (S. rebaudianaMS007) characteristicsand enhance target identi-fication processes to improve understanding of protein-protein interaction in S. re-baudianaMS007

In silico characterization of UGT74G1 protein in Stevia rebaudiana Bertoni Accession MS007

Stevia rebaudiana is being promoted as an alternative sweetener in particular for diabetic and obese patients due to its low-calorie property. The steady demand in the market for high-quality stevia extracts presents a challenge for enhanced production of steviol glycosides that are safe for human consumption. This study characterized the structure and content of gene involved in the production of UGT74G1 protein for S. rebaudiana accession MS007 through in silico analysis using transcriptome dataset of stevia MS007. Homologous search using BLASTp show high similarity to Q6VAA6 RecName: Full=UDP-glycosyltransferase 74G1 (S. rebaudiana) as the top hit sequences. InterPro family and domain protein motif search revealed the presence and entry of IPR002213 and IPR035595. The construction of the phylogenetic tree was done by selecting 19 out of 102 protein sequences from BLASTp. The phylogenetic analysis showed the same protein family which is Asteraceae. ProtParam Ex-Pasy, PSIPRED and Phyre2 computed the primary, secondary, and tertiary structures for UGT74G1 protein. The UGT74G1 predicted tertiary structure scored 100.0% confidence by the single highest scoring template and coverage of 96%. The model has dimensions (Å) of X: 57.609, Y: 70.386, and Z: 58.351. Outcomes of this research will help to enhance the understanding of UDP-glycosyltransferase 74G1 (S. rebaudiana MS007) characteristic and enhance target identification processes to improve understanding of protein-protein interaction in S. rebaudiana MS007

In silico analysis and 3d structure prediction of putative UDP-Glycosyltransfer-ase 76G1 protein in stevia Rebaudiana MS007

Stevia rebaudiana is a plant of the Asteraceae family that is used as a natural sweet-ener. Stevia has been shown to be safe for human consumption and has been utilised as a sweetener substitute for diabetic and obese people. In this study, the structure and gene content involved in the synthesis of putative UDP-glycosyltransferase 76G1 (UGT76G1) protein in S. rebaudiana MS007 were analysed using an in-silico method. Homologous search using BlastP revealed the highest percentage of identity, score, and E-value for UDP-glycosyltransferase 76G1-like of Helianthus annuus (ID: XP_021973845.1). The presence of IPR002213 UDP-glucuronosyl/UDP-glucosyl-transferase entry, which is available at locations 89bp to 246 bp, was also verified by the protein family search using InterPro. MEGA-X software was used to construct a molecular phylogeny study, revealing that this protein belongs to the Asteraceae fam-ily. To predict the primary, secondary, and tertiary protein structures of the putative UGT76G1 protein, the ProtParam, ExPasy, PSIPRED, and Phyre2 programmes were implemented. The putative UGT76G1 protein’s tertiary structure prediction was given a score of 100.0% confidence by the single highest scoring template and a coverage of 98%, with the dimension of the model being (Å) of X: 52.453, Y: 61.270, and Z: 48.102. The UGT76G1 model fulfilled the quality standards and was approved for further analysis after validation performed by PROCHECK, VERIFY3D, and ER-RAT. Thus, the findings of this work have contributed to a better knowledge of puta-tive UDP-glycosyltransferase 76G1 features and target recognition processes, which will lead to better information on protein-protein interaction in S. rebaudiana MS007

In Silico analysis and 3D structure prediction of putative UDP-Glycosyltransferase 76G1 protein in Stevia rebaudiana MS007

Stevia rebaudiana is a plant of the Asteraceae family that is used as a natural sweetener. Stevia has been shown to be safe for human consumption and has been utilised as a sweetener substitute for diabetic and obese people. In this study, the structure and gene content involved in the synthesis of putative UDP-glycosyltransferase 76G1 (UGT76G1) protein in S. rebaudiana MS007 were analysed using an in-silico method. Homologous search using BlastP revealed the highest percentage of identity, score, and E-value for UDP-glycosyltransferase 76G1-like of Helianthus annuus (ID:XP_021973845.1). The presence of IPR002213 UDP-glucuronosyl/UDP-glucosyltransferase entry, which is available at locations 89bp to 246 bp, was also verified by the protein family search using InterPro. MEGA-X software was used to construct a molecular phylogeny study, revealing that this protein belongs to the Asteraceae family. To predict the primary, secondary, and tertiary protein structures of the putative UGT76G1 protein, the ProtParam, ExPasy, PSIPRED, and Phyre2 programmes were implemented. The putative UGT76G1 protein’s tertiary structure prediction was given a score of 100.0% confidence by the single highest scoring template and a coverage of 98%, with the dimension of the model being (Å) of X: 52.453, Y: 61.270, and Z: 48.102. The UGT76G1 model fulfilled the quality standards and was approved for further analysis after validation performed by PROCHECK, VERIFY3D, and ERRAT. Thus, the findings of this work have contributed to a better knowledge of putative UDP-glycosyltransferase 76G1 features and target recognition processes, which will lead to better information on protein-protein interaction in S. rebaudiana MS007