3 research outputs found
2‑(4′-Aminophenyl)benzothiazole Labeled with <sup>99m</sup>Tc-Cyclopentadienyl for Imaging β‑Amyloid Plaques
The
development of a <sup>99m</sup>Tc-radiotracer for imaging of
β-amyloid (Aβ) plaques with single photon emission computed
tomography (SPECT) is strongly anticipated to provide a low cost and
broadly accessible diagnostic tool for Alzheimer’s disease
(AD). Within this framework, 2-(4′-aminophenyl)Âbenzothiazole,
known to display affinity and specificity for Aβ plaques, has
been joined to the tricarbonyl <i>fac</i>-[MÂ(CO)<sub>3</sub>]<sup>+</sup> (M = ReÂ(I), <sup>99m</sup>TcÂ(I)) core through the cyclopentadienyl
moiety to yield stable, neutral, and lipophilic complexes (<b>Re-1</b> and <sup><b>99m</b></sup><b>Tc-1</b>, respectively).
The <b>Re-1</b> complex was completely characterized with spectroscopic
methods and was shown to selectively stain Aβ plaques on sections
of human AD brain tissue. The <sup><b>99m</b></sup><b>Tc-1</b> complex displayed satisfactory initial brain uptake (0.53% ID/g
at 2 min) and <i>in vivo</i> stability in healthy mice,
while in transgenic 5xFAD mice, models for AD, a notable retention
in the brain was noted (1.94% ID/g at 90 min). The results are encouraging
and contribute to the effort of developing a SPECT amyloid imaging
agent
Synthesis and Characterization of <i>fac</i>-[M(CO)<sub>3</sub>(P)(OO)] and <i>cis-trans</i>-[M(CO)<sub>2</sub>(P)<sub>2</sub>(OO)] Complexes (M = Re, <sup>99m</sup>Tc) with Acetylacetone and Curcumin as OO Donor Bidentate Ligands
The
synthesis and characterization of neutral mixed ligand complexes <i>fac-</i>[MÂ(CO)<sub>3</sub>(P)Â(OO)] and <i>cis-trans</i>-[MÂ(CO)<sub>2</sub>(P)<sub>2</sub>(OO)] (M = Re, <sup>99m</sup>Tc),
with deprotonated acetylacetone or curcumin as the OO donor bidentate
ligands and a phosphine (triphenylphosphine or methyldiphenylphosphine)
as the monodentate P ligand, is described. The complexes were synthesized
through the corresponding <i>fac-</i>[MÂ(CO)<sub>3</sub>(H<sub>2</sub>O)Â(OO)] (M = Re, <sup>99m</sup>Tc) intermediate aqua complex.
In the presence of phosphine, replacement of the H<sub>2</sub>O molecule
of the intermediate complex at room temperature generates the neutral
tricarbonyl monophosphine <i>fac</i>-[ReÂ(CO)<sub>3</sub>(P)Â(OO)] complex, while under reflux conditions further replacement
of the trans to the phosphine carbonyl generates the new stable dicarbonyl
bisphosphine complex <i>cis-trans</i>-[ReÂ(CO)<sub>2</sub>(P)<sub>2</sub>(OO)]. The Re complexes were fully characterized by
elemental analysis, spectroscopic methods, and X-ray crystallography
showing a distorted octahedral geometry around Re. Both the monophosphine
and the bisphosphine complexes of curcumin show selective binding
to β-amyloid plaques of Alzheimer’s disease. At the <sup>99m</sup>Tc tracer level, the same type of complexes, <i>fac</i>-[<sup>99m</sup>TcÂ(CO)<sub>3</sub>(P)Â(OO)] and <i>cis-trans</i>-[<sup>99m</sup>TcÂ(CO)<sub>2</sub>(P)<sub>2</sub>(OO)], are formed
introducing new donor combinations for <sup>99m</sup>TcÂ(I). Overall,
β-diketonate and phosphine constitute a versatile ligand combination
for ReÂ(I) and <sup>99m</sup>TcÂ(I), and the successful employment of
the multipotent curcumin as β-diketone provides a solid example
of the pharmacological potential of this system
Synthesis and Characterization of <i>fac</i>-[M(CO)<sub>3</sub>(P)(OO)] and <i>cis-trans</i>-[M(CO)<sub>2</sub>(P)<sub>2</sub>(OO)] Complexes (M = Re, <sup>99m</sup>Tc) with Acetylacetone and Curcumin as OO Donor Bidentate Ligands
The
synthesis and characterization of neutral mixed ligand complexes <i>fac-</i>[MÂ(CO)<sub>3</sub>(P)Â(OO)] and <i>cis-trans</i>-[MÂ(CO)<sub>2</sub>(P)<sub>2</sub>(OO)] (M = Re, <sup>99m</sup>Tc),
with deprotonated acetylacetone or curcumin as the OO donor bidentate
ligands and a phosphine (triphenylphosphine or methyldiphenylphosphine)
as the monodentate P ligand, is described. The complexes were synthesized
through the corresponding <i>fac-</i>[MÂ(CO)<sub>3</sub>(H<sub>2</sub>O)Â(OO)] (M = Re, <sup>99m</sup>Tc) intermediate aqua complex.
In the presence of phosphine, replacement of the H<sub>2</sub>O molecule
of the intermediate complex at room temperature generates the neutral
tricarbonyl monophosphine <i>fac</i>-[ReÂ(CO)<sub>3</sub>(P)Â(OO)] complex, while under reflux conditions further replacement
of the trans to the phosphine carbonyl generates the new stable dicarbonyl
bisphosphine complex <i>cis-trans</i>-[ReÂ(CO)<sub>2</sub>(P)<sub>2</sub>(OO)]. The Re complexes were fully characterized by
elemental analysis, spectroscopic methods, and X-ray crystallography
showing a distorted octahedral geometry around Re. Both the monophosphine
and the bisphosphine complexes of curcumin show selective binding
to β-amyloid plaques of Alzheimer’s disease. At the <sup>99m</sup>Tc tracer level, the same type of complexes, <i>fac</i>-[<sup>99m</sup>TcÂ(CO)<sub>3</sub>(P)Â(OO)] and <i>cis-trans</i>-[<sup>99m</sup>TcÂ(CO)<sub>2</sub>(P)<sub>2</sub>(OO)], are formed
introducing new donor combinations for <sup>99m</sup>TcÂ(I). Overall,
β-diketonate and phosphine constitute a versatile ligand combination
for ReÂ(I) and <sup>99m</sup>TcÂ(I), and the successful employment of
the multipotent curcumin as β-diketone provides a solid example
of the pharmacological potential of this system