Additional file 1: Figure S1. of IMMUNEPOTENT CRP induces cell cycle arrest and caspase-independent regulated cell death in HeLa cells through reactive oxygen species production

(A) ROS levels were measured by flow cytometry through DCFDA staining in SiHa cells left alone or pretreated with NAC or QVD.oph and then treated with I-CRP (1.25 U/mL) for 24 h. (B) The effect on cell death of cells left alone or pretreated with NAC or QVD.oph and then treated with I-CRP (1.25 U/mL) for 24 h, was analyzed by flow cytometry through Annexin-V staining. The results were analyzed and graphed. (PDF 20 kb

Additional file 2: Figure S2. of IMMUNEPOTENT CRP induces cell cycle arrest and caspase-independent regulated cell death in HeLa cells through reactive oxygen species production

Left, caspase-3 activity of HeLa cells left untreated or pretreated with Nac, and then treated with I-CRP. Right, the results obtained were analyzed and graphed as the percentage of HeLa cells positive for caspase-3 activity. (PDF 37 kb