1 research outputs found
Near-Infrared Light-Directed RNAi Using a Photosensitive Carrier Molecule
Controlled
activation of small RNAs, such as small interfering
RNA, in cells is very useful for various biological applications.
Light is an effective inducer of controlled activation; in particular,
near-infrared light is favorable because it can penetrate deeper into
tissues than UV or visible light. In this study, near-infrared light
control of RNA interference (RNAi) was demonstrated in mammalian cells
using a photosensitive RNA carrier molecule, consisting of an RNA
carrier protein and a fluorochrome. The photosensitive carrier molecule
was identified from six candidates, each with a different fluorochrome.
Using this carrier molecule, cytosolic RNA delivery and RNAi can be
triggered by near-infrared light. Cytotoxicity was not observed after
photoinduction of RNAi