4 research outputs found

    Additional file 6: Table S2. of The genetic architecture of low-temperature adaptation in the wine yeast Saccharomyces cerevisiae

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    List of genes used in the RH analysis with the BY4741 strain that are present in the subtelomeric regions and are not essential. (XLSX 13 kb

    Additional file 3: Figure S2. of The genetic architecture of low-temperature adaptation in the wine yeast Saccharomyces cerevisiae

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    Workflow of populations’ selection and sequencing. Cells were grown in complete media (YPD) and synthetic must (SM), and were incubated at either optimum temperature (28 °C) or low temperature (15 °C) until the stationary phase was reached. At this time, the volume required to inoculate at an OD of 0.2 was re-inoculated into 60 mL of fresh medium. The experiment was carried out 8 times after which the selected populations were analyzed and sequenced. (PDF 43 kb

    Additional file 7: Figure S5. of The genetic architecture of low-temperature adaptation in the wine yeast Saccharomyces cerevisiae

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    Outline of the construction of advanced intercross lines. We carried out a strategy that forces yeast cells through multiple rounds of random mating and sporulation to create advanced intercross lines (AILs). This step can improve genetic mapping in two ways: increasing resolution by reducing linkage and unlinking nearby QTLs. (PDF 168 kb

    Additional file 2: Figure S1. of The genetic architecture of low-temperature adaptation in the wine yeast Saccharomyces cerevisiae

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    Distribution of private nonsynonymous SNPs in P5 and P24 compared to S288c. An external circle indicates P24 and an internal circle indicates P5. Homozygous changes are colored in green, while heterozygous changes are marked in red. (PDF 243 kb
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