5 research outputs found
Karyotype analysis of ES cells and live offspring derived from vitrified oocytes.
<p>A) Karyotype analysis performed on ViO-ES9 cells revealed a number of abnormalities. The male cell line has a chromosome count of 46. B) Karyotype analysis performed on the mice that were generated from vitrified oocytes revealed normal karyotypes, a representative karyotype for one of the male mice shows a normal 40 XY chromosome count with no abnormalities.</p
Survival, vitrified two-cell embryo and live offspring rates from either fresh or vitrified oocytes.
<p>Fisher's test indicated no significant differences between the treatments within same column (P>0.05).</p
Following in vitro fertilization and culture, both fresh (A1) and vitrified oocytes in 0.1 M trehalose (B1) were capable of developing to the two-cell (A2; B2), four-cell (A3; B3) and blastocyst (A4; B4) stages, respectively.
<p>Furthermore, blastocysts were able to hatch (A5; B5) without chemical or mechanical assistance and some were used to derive embryonic stem cells.</p
Survival, and live offspring rates for vitrified oocytes.
<p>Fisher's test indicated no significant differences between the treatments within same column (P>0.05). Oocyte and live offspring data collected from 5 and 2 replicates respectively.</p