37 research outputs found
DataSheet1_Mechanistic insights into reductive deamination with hydrosilanes catalyzed by B(C6F5)3: A DFT study.docx
Selective defunctionalization of synthetic intermediates is a valuable approach in organic synthesis. Here, we present a theoretical study on the recently developed B(C6F5)3/hydrosilane-mediated reductive deamination reaction of primary amines. Our computational results provide important insights into the reaction mechanism, including the active intermediate, the competing reactions of the active intermediate, the role of excess hydrosilane, and the origin of chemoselectivity. Moreover, the study on the substituent effect of hydrosilane indicated a potential way to improve the efficiency of the reductive deamination reaction.</p
LocateP: Genome-scale subcellular-location predictor for bacterial proteins-1
By 2D gel electrophoresis) which have a putative SPI-cleavage site motif in the C-region that follows the transmembrane helix H-region (see Fig. 1B). A sequence composition chart, made using WebLogo [47], based on multiple-sequence alignment of the H- and C-regions (see Fig. 1B) of the N-anchored and secreted protein sets. The red arrow indicates the cleavage position of true SPI-site motifs (see Figure 1B), and the green dashed arrow represents the corresponding position in N-anchored proteins that is not cleaved. The specificity of HMMs of different lengths containing the putative cleavage site A* = the Alanine after which cleavage takes place. Mod1: residues -9 to A*; Mod2: residues -11 to A*; Mod3: residues -14 to A*; Mod4: residues -8 to +3 of A*; Mod5: residues -13 to +10 of A*; Mod6: residues -8 to +17 of A*; Mod7: residues -3 to +10 of A*; Mod8: residues -3 to +17 of A*; Mod9: residues +1 to +25.<p><b>Copyright information:</b></p><p>Taken from "LocateP: Genome-scale subcellular-location predictor for bacterial proteins"</p><p>http://www.biomedcentral.com/1471-2105/9/173</p><p>BMC Bioinformatics 2008;9():173-173.</p><p>Published online 27 Mar 2008</p><p>PMCID:PMC2375117.</p><p></p
LocateP: Genome-scale subcellular-location predictor for bacterial proteins-2
by combining Tat-find v1.2 [91] and our Tat-specific HMMs (RR-HMM, CS-HMM). Bacteriocin-like proteins were identified using Bagel [149]. Secondly, Phobius [14], PrediSi [98], SignalP 3.0 [18] and TMHMM 2.0 [12] were combined to identify transmembrane regions. Those proteins without any predicted TM segments were considered intracellular, whereas those with TM segments were divided into multi-TM membrane proteins, N-anchored membrane proteins or secreted/released proteins (single N-terminal TM segment, possibly signal peptide), and C-anchored membrane proteins (signal peptide and single C-terminal TM segment). Thirdly, a sortase-substrate HMM [165] was used to distinguish LPxTG-type peptidoglycan-anchored proteins from C-anchored membrane proteins. Subsequently, signal peptidase type II (SPII) substrates were predicted by combining existing lipoprotein motif models [41, 157] and new lipoprotein HMMs. The remaining proteins were classified into the categories secreted/released or N-anchored membrane proteins. See Methods and additional file for more details. Abbreviation: A-S = Anchored-Secreted; TMS = TransMembrane Segment; SP = Signal Peptide; C/N-TM = C/N-terminally transmembrane anchored; LPxTG = LPxTG cell-wall anchored.<p><b>Copyright information:</b></p><p>Taken from "LocateP: Genome-scale subcellular-location predictor for bacterial proteins"</p><p>http://www.biomedcentral.com/1471-2105/9/173</p><p>BMC Bioinformatics 2008;9():173-173.</p><p>Published online 27 Mar 2008</p><p>PMCID:PMC2375117.</p><p></p
MOESM1 of Regulators of plant biomass degradation in ascomycetous fungi
Additional file 1. Prevalence of transcription factors in Fungi
MOESM3 of Regulators of plant biomass degradation in ascomycetous fungi
Additional file 3. Maximum likelihood tree of Cys2Hys2 transcription factors (TFs). In bold are the species for which the TF has been characterized, while in bold and larger font are the species in which the TF was first discovered
MOESM2 of Regulators of plant biomass degradation in ascomycetous fungi
Additional file 2. Maximum likelihood tree of Zn2Cys6 transcription factors (TFs). In bold are the species for which the TF has been characterized, while in bold and larger font are the species in which the TF was first discovered
MOESM5 of Regulators of plant biomass degradation in ascomycetous fungi
Additional file 5. Maximum likelihood tree of bHLH (XPP1) transcription factors (TFs). In are the species for which the TF has been characterized, while in bold and larger font are the species in which the TF was first discovered
Cytotoxic withanolides from <i>Physalis angulata</i>
<p>A new withanolide (<b>1</b>), physagulide P, together with five known withanolides (<b>2–6</b>), was isolated from the aerial parts of <i>Physalis angulata</i> L. The structure of new compound was elucidated on the basis of extensive spectroscopic techniques, including 1D, 2D NMR and HRESIMS. The activity screening indicated that compound <b>1</b> showed significant cytotoxicities against the human osteosarcoma cell line MG-63, HepG-2 hepatoma cells and breast cancer cells MDA-MB-231 with the IC<sub>50</sub> value of 3.50, 4.22 and 15.74 μM.</p
MOESM4 of Regulators of plant biomass degradation in ascomycetous fungi
Additional file 4. Maximum likelihood tree of MADS BOX (McmA) transcription factors (TFs). In bold are the species for which the TF has been characterized, while in bold and larger font are the species in which the TF was first discovered
Synthesis of Sterically Protected Xanthene Dyes with Bulky Groups at C‑3′ and C‑7′
Substitution of the xanthene scaffold
with bulky groups at C-3′
and C-7′ is expected to protect the electrophilic central methine
carbon against nucleophilic attack and inhibit stacking. However,
such structures are not readily prepared via traditional xanthene
syntheses. We have devised an alternative and convenient synthesis
to enable facile preparation of this subset of xanthene dyes under
mild conditions and in good yields