2 research outputs found
Ion Permeability of Polydopamine Films Revealed Using a Prussian Blue-Based Electrochemical Method
Polydopamine
(PDA) is fast becoming a popular surface modification
technique. Detailed understanding of the ion permeability properties
of PDA films will improve their applications. Herein, we report for
the first time the thickness-independent ion permeability of PDA films
using a Prussian blue (PB)-based electrochemical method. In this method,
PDA films are deposited via ammonium persulfate-induced dopamine polymerization
onto a PB electrode. The ion permeability of the PDA films can thus
be detected by observing the changes in electrochemical behaviors
of the PB coated by PDA films. On the basis of this method, it was
unexpectedly found that the PDA films with thickness greater than
45 nm (e.g., ∼60 and ∼113 nm) can exhibit pH-switchable
but thickness-insensitive permeability to monovalent cations such
as potassium and sodium ions. These observations clearly indicate
the presence of a continuous network of interconnected intermolecular
voids within PDA films, regardless of film thickness
Functional Toxicogenomic Assessment of Triclosan in Human HepG2 Cells Using Genome-Wide CRISPR-Cas9 Screening
There
are thousands of chemicals used by humans and detected in
the environment for which limited or no toxicological data are available.
Rapid and cost-effective approaches for assessing the toxicological
properties of chemicals are needed. We used CRISPR-Cas9 functional
genomic screening to identify the potential molecular mechanism of
a widely used antimicrobial triclosan (TCS) in HepG2 cells. Resistant
genes at IC50 (the concentration causing a 50% reduction in cell viability)
were significantly enriched in the adherens junction pathway, MAPK
signaling pathway, and PPAR signaling pathway, suggesting a potential
role in the molecular mechanism of TCS-induced cytotoxicity. Evaluation
of the top-ranked resistant genes, <i>FTO</i> (encoding
an mRNA demethylase) and <i>MAP2K3</i> (a MAP kinase kinase
family gene), revealed that their loss conferred resistance to TCS.
In contrast, sensitive genes at IC10 and IC20 were specifically enriched
in pathways involved with immune responses, which was concordant with
transcriptomic profiling of TCS at concentrations o