Genexput: A rapid and standarised method for large genome expansion of Pseudomonas putida

The soil bacteria Pseudomonas putida has gained considerable interest in recent years due to its metabolic complexity, which gives the bacteria huge potential in fields as diverse as bioremediation and bio-based industrial production of chemicals [1]. However, the full exploitation of P. putida potential requires of the development of standarised and hightrouput large genome edditing methods in this bacterium. Actual synbiotools for this purpose in P. putida are still in its infancy and need to be optimised. For instance, well-known state-of-the-art methods such as recombineering and CRISPR-Cas approachs have yet low efficiency in this bacteria [2]. In an attemp to address this important shorcoming in the portfolio of P. putida synbiotools, we have constructed a pSEVA-based [3] library of vectors which allows the genome integration via homologous recombination of large fragments of exogenous DNA. Thereof, loci distributed all along the genome were selected and designed as landing paths for the integration of alien DNA. This way it is possible to add an additional level of gene expression control at genome localization level. We further validated our approach by i) monitoring the expression level of a single GFP reported gene along the genome localization and ii) by expanding the metabolic versatility of P.putida KT2440 towards toluene and m-xylene by integrating in its chromosome the genes encoded in the pWWO plasmid. In summary we provide an efficient toolkit that allows a rapid genome expansion of P. putida while allowing the expression level of the desired transgene

Método integral para la gestión de la producción de lechería especializada, en el trópico alto cundinamarqués : documento técnico de soporte

Libro que establece un método integral para la gestión de la producción de leche especializada en fincas de productores pertenecientes al trópico alto cundinamarqués producto del análisis del sistema de producción de leche cruda de la finca San Pedro del Centro de Biotecnología (CBA) del SENA.Book that establishes a comprehensive method for the management of specialized milk production in farms of producers belonging to the high Cundinamarqués tropic product of the analysis of the raw milk production system of the San Pedro farm of the Center for Biotechnology (CBA) of SENA.Caracterización y modelamiento de la unidad de producción lechera -- Marco teórico -- Diseño metodológico -- Caracterización de la unidad de producción de leche -- Modelamiento de la unidad de producción lechera -- Análisis del comportamiento del sistema actual -- Identificación de estrategias de mejora del proceso de producción -- Evaluación de las estrategias de mejora del proceso de producción -- Desarrollo del método integrador para la gestión de la producción lechera -- El mapa de procesos de la producción de leche en su finca -- Rotación de potreros: alimentando a su ganado de la mejor manera y al mejor precio posible -- Desarrollo del modelo de control permanente o Cuadro de Mando Integral (CMI)na179 página

Locating Two Transfer Points on a Network with a Trip Covering Criterion and Mixed Distances

In this paper we consider a set of origin-destination pairs in a mixed model in which a network embedded in the plane represents an alternative high-speed transportation system, and study a trip covering problem which consists on locating two points in the network which maximize the number of covered pairs, that is, the number of pairs which use the network by acceding and exiting through such points. To deal with the absence of convexity of this mixed distance function we propose a decomposition method based on formulating a collection of subproblems and solving each of them via discretization of the solution set.Ministerio de Educación, Ciencia e Innovación MTM2009-14243Ministerio de Economía y Competitividad MTM2012-37048Junta de Andalucía P09-TEP-5022Junta de Andalucía P10-FQM-584

Optimal Alignments for Designing Urban Transport Systems: Application to Seville

The achievement of some of the Sustainable Development Goals (SDGs) from the recent 2030 Agenda for Sustainable Development has drawn the attention of many countries towards urban transport networks. Mathematical modeling constitutes an analytical tool for the formal description of a transportation system whereby it facilitates the introduction of variables and the definition of objectives to be optimized. One of the stages of the methodology followed in the design of urban transit systems starts with the determination of corridors to optimize the population covered by the system whilst taking into account the mobility patterns of potential users and the time saved when the public network is used instead of private means of transport. Since the capture of users occurs at stations, it seems reasonable to consider an extensive and homogeneous set of candidate sites evaluated according to the parameters considered (such as pedestrian population captured and destination preferences) and to select subsets of stations so that alignments can take place. The application of optimization procedures that decide the sequence of nodes composing the alignment can produce zigzagging corridors, which are less appropriate for the design of a single line. The main aim of this work is to include a new criterion to avoid the zigzag effect when the alignment is about to be determined. For this purpose, a curvature concept for polygonal lines is introduced, and its performance is analyzed when criteria of maximizing coverage and minimizing curvature are combined in the same design algorithm. The results show the application of the mathematical model presented for a real case in the city of Seville in Spain.Ministerio de Economía y Competitividad MTM2015-67706-

A general approach for the location of transfer points on a network with a trip covering criterion and mixed distances

In this paper we consider a trip covering location model in a mixed planar-network space. An embed- ded network in the plane represents an alternative transportation system in which traveling is fasterthan traveling within the plane. We assume that the demand to be covered is given by a set of origin- destination pairs in the plane, with some traffic between them. An origin-destination pair is covered bytwo facility points on the network (or transfer points), if the travel time from the origin to destinationby using the network through such points is not higher than a given acceptance level related to the traveltime without using the network. The facility location problems studied in this work consist of locatingone or two transfer points on the network such that, under several objective functions, the traffic throughthe network is maximized. Due to the continuous nature of these problems, a general approach is pro- posed for discretizing them. Since the non-convexity of the distance function on cyclic networks alsoimplies the absence of convexity of the mixed distance function, such an approach is based on a decom- position process which leads to a collection of subproblems whose solution set can be found by adaptingthe general strategy to each problem considered.Ministerio de Economía y Competitividad MTM2012-37048Ministerio de Economía y Competitividad MTM2015-67706-PJunta de Andalucía P10-FQM-584

El modelo de gestión socioeconómico como elemento canalizador del potencial de las personas en la transformación interna y externa en el contexto de la creación y consolidación de las organizaciones

Es aceptado por la literatura académica, entre los factores determinantes, la importancia de los recursos humanos en la generación de valor y en la continuidad a lo largo del tiempo de las diferentes organizaciones en el mercado. Es por ello, que el modelo de gestión socioeconómico, desarrollado por el profesor Henri Savall, de la Universidad Jean Moulin de Lyon, señala como elemento clave la gestión de los recursos humanos como eje central del cambio organizacional que posibilita y fomenta las relaciones laborales sanas. Basado en la adquisición de nuevas competencias gerenciales y la cooperación entre empleadores y trabajadores. Todo lo anterior permitirán minimizar los disfuncionamientos que en toda organización se producen como resultado de la iteración en el día a día de las personas con lo las llamadas estructuras organizacionales. Esta diferencia, en muchos casos muy marcada, entre el comportamiento observado y el esperado de cada uno de los trabajadores que conforman la organización generan implicaciones muy severas sobre el funcionamientos organizacional. Hasta el punto de determinar la diferencia entre la supervivencia y permanencia en el mercado de las empresas o su desaparición definitiva. Mediante el estudio del caso sobre una entidad de carácter mercantil, profundizaremos en los resultados alcanzados mediante la aplicación del modelo de gestión socioeconómico a dicha organización. Concluiremos como dicha implementación redundará en un cambio organizacional, basado en la adquisición de nuevas competencias, favoreciendo y propiciando la cooperación y compromiso organizacional de los miembros de las empresas. Poniéndose de relieve la hipótesis fundamental que todo empleado relacionado con la organización puede ejercer su poder no formal para favorecerla o perjudicarla. Como consecuencia y reflejo del papel determinante que poseen las actitudes y aptitudes desarrolladas y adquiridas por cada uno de ellos como motor de dicho poder.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech

Improved results for the k-centrum straight-line location problem

The k-Centrum problem consists in finding a point that minimises the sum of the distances to the k farthest points out of a set of given points. It encloses as particular cases to two of the most known problems in Location Analysis: the center, also named as the minimum enclosing circle, and the median. In this paper the k-Centrum criteria is applied to obtaining a straight line-shaped facility. A reduced finite dominant set is determined and an algorithm with lower complexity than the previous one obtained.Ministerio de Ciencia y Tecnologí

La persistencia del recuerdo

Tras un análisis teórico-conceptual de la obra, presento mi propuesta artística que tiene cómo objetivo construir una serie de imágenes fotográficas de lugares a través de la fotografía, en los que no se pueda vislumbrar con claridad lo que en ellas aparece, utilizando un lenguaje basado en la abstracción, creando composiciones estéticas basadas en el dinamismo de la interacción entre la forma y el color

Distribución óptima del transporte intermodal: aplicación a la exportación de perecederos

This paper discusses the benefits using intermodal transport for fruit and vegetables. Multi-Criteria Decision Making techniques are applied for optimal allocation between land or intermodal transport. Also a sensitivity analysis is made for knowing changing priorities in decision-makers (exporters) to encourage a modal shift. The results show savings in intermodal transport costs in relation to land transport cost. However the exporter must assume increased time of delivery of goods.Agribusiness, Agricultural and Food Policy, F14, Q17,