Body weight and survival rate of mice following lethal challenge with influenza virus.

<p>At 18 days following the last immunization, all mice in study group B were intranasally challenged with 10 LD₅₀ of H1N1/A/TN/1-560/2009-MA2 virus. (A) Body weight and (B) survival of mice orally immunized with PBS, LL-HA1/L/AcmA or HA1/L/AcmA, or subcutaneously immunized with HA1/L/AcmA-FA, was monitored daily for 14 days following virus challenge. The body weight of mice on the day of viral challenge was used as the baseline weight to determine the body weight changes post-viral challenge. Asterisks indicate statistically significant differences in comparison with the PBS-treated group (*p<0.05; **p<0.01; ***p<0.001).</p

Binding optimization of HA1/L/AcmA recombinant protein to <i>L</i>. <i>lactis</i>.

<p>(A) The percentage of <i>L</i>. <i>lactis</i> surface displaying HA1/L/AcmA recombinant protein after incubation with different amounts of HA1/L/AcmA recombinant protein. (B) The percentage of <i>L</i>. <i>lactis</i> surface displaying HA1/L/AcmA recombinant protein after incubation with HA1/L/AcmA recombinant protein for 1 h, 2 h, 3 h, and 4 h, respectively. (C) The percentage and mean fluorescence intensity (MFI) value of <i>L</i>. <i>lactis</i> surface displaying HA1/L/AcmA recombinant protein after incubation with HA1/L/AcmA recombinant protein in GM17 and PBS, respectively. The data represents mean ± standard deviation. Asterisk indicates statistically significant differences between groups (**P<0.01).</p

Body weight and survival rate of immunized mice upon lethal challenge with influenza virus.

<p>Body weight and survival rate of immunized mice upon lethal challenge with influenza virus.</p

Additional file 2: of Emergence of the Asian lineage dengue virus type 3 genotype III in Malaysia

Phylogeography of DENV3/III-C. (KML 1198 kb

Additional file 1: of Emergence of the Asian lineage dengue virus type 3 genotype III in Malaysia

Table S1. Dengue virus type 3 strains used in analysis in this study. (DOCX 44 kb