Influence of group, environment, season and size on feeding behaviour.

<p>Distribution of the three classes of feeding behaviour (invertebrate eaters in purple, omnivores in orange and diatom eaters in deep green). Stacked bar chart showing the proportions of the various feeding behaviour classes by species and zone type in <b>A</b>), by season for each syntopic zone in <b>B</b>). Probability of GutVac (<i>y</i>-axis) as predicted by the multinomial model as a function of size in <b>C)</b> in which the <i>X</i>-axis corresponds to body size in cm, and we can read for example the longer specimens are more omnivor than herbivor.</p

Feeding behaviour.

<p>Molecular detection in the feces of the eight prey items analyzed by a principal component analysis of proportions. <b>A)</b> Principal plane of the PCA. The reference populations are represented by closed triangles for the Chée River (Cn. allopatry), open triangles for the Allier (Cn allopatry), closed diamonds for the Suran (Pt allopatry), and open diamonds for the Orbieu (Pt allopatry). The syntopic zones are indicated by a closed square for the Ardèche basin and a closed circle for the Durance basin. <i>C</i>. <i>nasus</i> is shown in green, <i>P</i>. <i>toxostoma</i> in red, hybrids in blue and <i>Barbus barbus</i> (insectivorous control) in grey. The size of the symbols for each specimen is proportional to the number of prey items detected. <b>B)</b> Non-parametric density estimation (Gaussian kernel) of the first axis co-ordinates. Each color corresponds to a category as follows: in purple, invertebrate eaters (i.e. only invertebrates detected in feces); in orange, omnivores (both invertebrates and diatoms detected); and, in deep green, diatom eaters (only diatoms were detected). The distribution density corresponding to <i>Barbus barbus</i> specimens is indicated by grey dashes.</p

Number of specimens considered, by analysis.

<p>K = coefficient of condition, Morpho = morphological analysis of the body, Diet = vacuity analysis and in parenthesis the DNA barcoding sub-sample. <i>Pt</i> = <i>P</i>. <i>toxostoma</i>, <i>Cn</i> = <i>C</i>. <i>nasus</i>, Sur = Suran, Ber = Berre, Orb = Orbieu, Che = Chée, All = Allier, Mir = Miribel, Ron = Roussillon, Ros = Rosières, Bau = Labeaume, Jus = Saint-Just, Bue = Buech, Man = Manosque, Per = Pertuis, Avi = Avignon, Spc = Serre-Ponçon. Data previously published are in italics [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0142592#pone.0142592.ref029" target="_blank">29</a>,<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0142592#pone.0142592.ref034" target="_blank">34</a>], new data are in bold.</p><p>All specimens were genotyped, and only subgroups of specimens were used in the various analyses, because size, weight measurements, images of the body or feces was unavailable.</p

Integrative ecological pattern for the <i>Chondrostoma</i> complex in the two syntopic zones.

<p>Environmental parameters, such as water level, perillithon availability and clogging, are shown diagrammatically, with the distribution of the three genomic groups over the upstream-downstream gradient of the rivers. Morphological analysis, feeding behaviour, feeding activity, the coefficient of condition and growth rate are shown schematically in the three boxes. In the Ardèche, the responses of the three genomic groups differed. By contrast, in the Durance basin, the three genomic groups displayed a similar tendency.</p

Sampling map.

<p>Four allopatric stations (red for <i>P</i>. <i>toxostoma</i> and dark green for <i>C</i>. <i>nasus</i>) and 12 sympatric stations (the Rhône basin) were sampled. Furthermore, sympatric populations classified as allotopic stations (five; orange for <i>P</i>. <i>toxostoma</i> and light green for <i>C</i>. <i>nasus</i>) and syntopic station (blue stations; three for the Ardèche basin and four for the Durance basin). Allopatric and alltopic populations constituted the reference populations. This picture is a modified version of a copyright free picture of Daniel Dalet, available on <a href="http://www.histgeo.ac-aix-marseille.fr/" target="_blank">www.histgeo.ac-aix-marseille.fr</a>.</p

Summary of body deformations taking into account species, size, environment and their interaction.

<p>A) Ontogenic deformations f<i>or C</i>. <i>nasus</i> in reference condition, B) Ontogenic deformations f<i>or P</i>. <i>toxostoma</i> in reference condition, C) Interspecies deformations in reference condition, D) Deformation from reference condition to Durance for both species, E) Deformation from reference condition to Ardèche for both species. See <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0142592#pone.0142592.s005" target="_blank">S5 Fig</a>. for more details. The width of an arrow is positively related to the intensity of the deformation.</p