UTJECAJ INOKULATA MLIJEČNO KISELIH BAKTERIJA NA FERMENTACIJU I AEROBNU STABILNOST SILAŽE SUNCOKRETA

This study was carried out to determine the effects of actic acid bacterial inoculant on the fermentation and aerobic stability of sunflower silages. Sunflower was harvested at the milk stage. Inoculant-1174 (Pioneer®,USA) was used as homofermentative lactic acid bacterial inoculant. Inoculant was applied 6.00 log10 cfu/g silage levels. Silages with no additive served as controls. After treatment, the chopped sunflower was ensiled in the PVC type laboratory silos. Three silos for each group were sampled for chemical and microbiological analysis on days 2, 4, 7, 14, 21, 28 and 56 after ensiling. At the end of the ensiling period, all silages were subjected to an aerobic stability test for 14 days. Neither inoculant improved the fermentation parameters of sunflower silages. At the end of the ensiling period, inoculant increased lactic acid bacteria (LAB) and decreased yeast and mould numbers of silages. Inoculant treatment did not affect aerobic stability of silages.Ovo istraživanje imalo je za cilj odrediti utjecaj inokulata mliječno kiselih bakterija na fermentaciju i aerobnu stabilnost silaža suncokreta. Žetva suncokreta obavljena je u mliječnoj zriobi. Inokulat-1174 (PioneerR, USA) koristio se kao homofermentativni inokulat mliječno kiselih bakterija. Inokulat primijenjen je na razinama 6.00 log 10 cfu/g. Kontrole su bile silaže bez aditiva. Nakon tretiranja, sjeckani je suncokret siliran u PVC laboratorijskim silosima. Uzorci su uzimani iz 3 silosa za svaku grupu te kemijski i mikrobiološki analizirani 2., 4., 7., 14., 21., 28. i 56. dana nakon siliranja. Na kraju siliranja sve su silaže testirane na aerobnu stabilnost u periodu od 14 dana. Inokulat nije poboljšao fermentacijske parametre silaža suncokreta. Na kraju procesa siliranja inokulat je povećao mliječno kisele bakterije (LAB), a smanjio kvasac i plijesan u silažama. Postupak inokulatom nije utjecao na aerobnu stabilnost silaža

THE EFFECTS OF INOCULANT LACTIC ACID BACTERIA ON THE FERMENTATION AND AEROBIC STABILITY OF SUNFLOWER SILAGE

This study was carried out to determine the effects of actic acid bacterial inoculant on the fermentation and aerobic stability of sunflower silages. Sunflower was harvested at the milk stage. Inoculant-1174 (Pioneer®,USA) was used as homofermentative lactic acid bacterial inoculant. Inoculant was applied 6.00 log10 cfu/g silage levels. Silages with no additive served as controls. After treatment, the chopped sunflower was ensiled in the PVC type laboratory silos. Three silos for each group were sampled for chemical and microbiological analysis on days 2, 4, 7, 14, 21, 28 and 56 after ensiling. At the end of the ensiling period, all silages were subjected to an aerobic stability test for 14 days. Neither inoculant improved the fermentation parameters of sunflower silages. At the end of the ensiling period, inoculant increased lactic acid bacteria (LAB) and decreased yeast and mould numbers of silages. Inoculant treatment did not affect aerobic stability of silages

The Ensiling Capability of Various Commercial Mixture Additives on Sunflower Silage Composition, Organic Matter Digestibility and Microbial Profiles

The objective of this study was to evaluate the effects of commercial acid mixtures on silage chemical composition, lactobacillus, yeast and mold in the silages and organic matter digestibility. The treatment groups as follows: (1) Control (C, no additive), (2) formic acid, propionic acid and lignosulphopic acid mixture (1 g/kg, SA, soft acid (R)), (3) calcium formiate, sodium benzoate and sodium metabisulfite mixture (1 g/kg, Silamix (R)) and (4) SA+SM mixture (1 g/kg SA+1 g/kg SM) admixed to fresh sunflower crops. Then, fresh crop materials were filled one kg in vacuum nylons and vacuumed with six replicates and kept for 90 days at room temperature (about 20 to 26 degrees C). Dry matter content of silages were lower in the SA+SM supplemented group than those of C and SA groups and crude protein content of SM and the SA+SM groups lower than those of C and SA groups (P<0.01). The crude fiber and acid detergent fiber ratio were higher in the C group than the other groups (P<0.01). In the C group the HEM content were lower than SA+SM group (P<0.05) and in the SA group's ADL were higher than those of other groups (P<0.01). Acids supplementations increased total digestibility nutrient ratio. The lactic acid concentration in the SA+SM group were higher than those of other groups and in the SM group, organic matter digestibility were higher than those of C and SA groups (P<0.01). Acid addition was not affect pH, ether extract, NDF, and lactobacillus and yeast count of silages. In conclusion, acids mixture supplementation to sunflower silage caused a decrease in DM, CP and ADF content, however, increased HEM, TDN, LA and OMD and so they may improve silage quality