Novel pyridinium surfactants for efficient, nontoxic in vitro gene delivery

Novel, double-chained pyridinium compounds have been developed that display highly efficient DNA transfection properties. The transfection efficiency of several of these compounds is enhanced by an order of magnitude, when compared with the transfection efficiency accomplished with the widely used cationic lipid system, lipofectin. Most importantly, the pyridinium compounds were found to be essentially nontoxic toward cells. Using various reporter genes, such as beta-galactosidase and pNEO (a gene construct that renders cells resistent to antibiotic derivatives of neomycin like G418), we demonstrate that the enhanced efficiency relates to the fact that a relative higher number of cells in the population is transfected (approximately 50% in the case of COS cells) by the pyridinium derivatives, whereas the delivery of DNA per cell is also enhanced. Furthermore, application of the pyridinium derivatives shows little cellular preference in their ability to transfect cells. By systematically modifying the structure of the pyridinium amphiphile, i.e., by changing either the headgroup structure or the alkyl chains, some insight was obtained that may lead to unraveling the mechanism of amphiphile-mediated transfection, and thus to protocols that further optimize the carrier properties of the amphiphile. Our results reveal that unsaturated alkyl chains enhance the transfection properties of the pyridinium-based amphiphiles. Preliminary experiments suggest that the structure-dependent improvement of transfection efficiency, when comparing pyridinium derivatives with lipofectin, likely relates to the mechanism of delivery rather than the packaging of the amphiphile/DNA complex

Work placement as an effective learning experience for undergraduate students: work-based learning and professional competencies; reflection by case study, 49th ASC Annual International Conference Proceedings. http://ascpro0.ascweb.org/archives/cd/2013/pa

Work Placement has been regarded as an important feature of undergraduate education. This paper reports on findings from a research project that reflected upon the effectiveness of the work placement process of third year Construction Management undergraduates, enrolled in the academic year 2011-2012 in the Dublin Institute of Technology (DIT) Construction Management Program. The project was undertaken to reflect upon the work placement process in order to determine the effectiveness of the module and its contribution to professional competencies development. The Kolb Learning Styles Model was applied to capture the student experiences and to provide a basis for observation and reflection. The research adopted a qualitative methodology and gathered the perspectives of students and supervisors (faculty) involved in the work placement process. Interviews and questionnaires were used to collect the data, which were assessed against the learning outcomes and the Construction Management Program structure. The key findings of this research suggest that the overall work placement process is an effective student centred learning experience and an excellent way of expanding the student's knowledge in the construction area within the DIT Construction Management Program

Truchet-tile structure of a topologically aperiodic metal–organic framework

When tiles decorated to lower their symmetry are joined together, they can form aperiodic and labyrinthine patterns. Such Truchet tilings offer an efficient mechanism of visual data storage related to that used in barcodes and QR codes. We show that the crystalline metal–organic framework [OZn4][1,3-benzenedicarboxylate]3 (TRUMOF-1) is an atomic-scale realization of a complex three-dimensional Truchet tiling. Its crystal structure consists of a periodically arranged assembly of identical zinc-containing clusters connected uniformly in a well-defined but disordered fashion to give a topologically aperiodic microporous network. We suggest that this unusual structure emerges as a consequence of geometric frustration in the chemical building units from which it is assembled

Truchet-tile structure of a topologically aperiodic metal–organic framework

When tiles decorated to lower their symmetry are joined together, they can form aperiodic and labyrinthine patterns. Such Truchet tilings offer an efficient mechanism of visual data storage related to that used in barcodes and QR codes. We show that the crystalline metal–organic framework [OZn4][1,3-benzenedicarboxylate]3 (TRUMOF-1) is an atomic-scale realization of a complex three-dimensional Truchet tiling. Its crystal structure consists of a periodically arranged assembly of identical zinc-containing clusters connected uniformly in a well-defined but disordered fashion to give a topologically aperiodic microporous network. We suggest that this unusual structure emerges as a consequence of geometric frustration in the chemical building units from which it is assembled