Use of AFLP and RAPD molecular genetic markers and cytogenetic analysis to explore relationships among taxa of the Patagonian Bromus setifolius complex

Bromus setifolius var. pictus (Hook) Skottsb., B. setifolius var. setifolius Presl. and B.setifolius var. brevifolius Ness are three native Patagonian taxa in the section Pnigma Dumort of the genus Bromus L. AFLP and RAPD analysis, in conjunction with genetic distance measurements and statistical techniques, revealed variation within this group and indicated that B. setifolius var. brevifolius was closely related to B. setifolius var. pictus, with both taxa being more distantly related to B. setifolius var. setifolius. Cytogenetic analysis confirmed the chromosomal number of B. setifolius var. pictus (2n = 70) and B. setifolius var. setifolius (2n = 28) and showed for the first time that B. setifolius var. brevifolius had 2n = 70. The combination of molecular genetic and cytogenetic evidence supported a species status for two of the three taxa and suggested hypotheses for the evolutionary origin of these complex taxa. Species status was also indicated for B. setifolius var. setifolius. Based on these findings, we suggest that B. setifolius var. pictus be referred to as B. pictus Hook var. pictus, and B. setifolius var brevifolius as B. pictus Hook var brevifolius. The correlation between AFLP diversity and variation in ecological parameters suggested that this marker system could be used to assess breeding progress and to monitor the domestication of Patagonian Bromus species for agronomic use

THE SPECIES OF THE GENUS ARISTIDA L. POACEAE IN CHILE

Volume: 44Start Page: 17End Page: 2

THE SPECIES OF THE GENUS PANICUM L. POACEAE IN CHILE

Volume: 44Start Page: 25End Page: 3

WEEDS OF THE FAMILY ASTERACEAE NEW FOR THE FLORA OF CHILE

Volume: 47Start Page: 57End Page: 6

NEW SPECIES FOR THE ADVENTITIOUS FLORA OF CHILE

Volume: 46Start Page: 209End Page: 21

CONTRIBUTION TO THE HIGH ELEVATION FLORA OF THE CHILEAN PATAGONIA A CHECKLIST OF SPECIES ON MOUNTAINS ON AN EAST-WEST TRANSECT IN THE SIERRA DE LOS BAGUALES LATITUDE 50 DEGREES SOUTH

Volume: 46Start Page: 121End Page: 15

New records for the flora of Chile, based on collections from the Second Region

Volume: 55Start Page: 17End Page: 2