MOESM3 of Differences in amino acid frequency in CagA and VacA sequences of Helicobacter pylori distinguish gastric cancer from gastric MALT lymphoma

Additional file 3: Figure S2. Phylogenetic tree of only 18 GC and 12 MALT strains. The star-like topology of this tree implies that these strains are genetically homogenous, and that their population has no clear structure (NJ-tree; Kimura-2 parameters; MEGA v. 6.0)

MOESM1 of Differences in amino acid frequency in CagA and VacA sequences of Helicobacter pylori distinguish gastric cancer from gastric MALT lymphoma

Additional file 1: Table S1. Accession numbers of the cagA and vacA gene sequences

Univariate and multivariate analyses of differentiated and undifferentiated gastric cancer groups.

Univariate and multivariate analyses of differentiated and undifferentiated gastric cancer groups.</p

Background of patients with gastric cancer following <i>H</i>. <i>pylori</i> eradication.

Background of patients with gastric cancer following H. pylori eradication.</p

Histological findings of differentiated gastric cancer after <i>H</i>. <i>pylori</i> eradication.

Histological findings of differentiated gastric cancer after H. pylori eradication.</p

Comparison between differentiated and undifferentiated adenocarcinoma after <i>H</i>. <i>pylori</i> eradication.

Comparison between differentiated and undifferentiated adenocarcinoma after H. pylori eradication.</p

Three portions are defined by subdividing both the lesser and the greater curvatures into three equal lengths.

U: Upper third, M: Middle third, L: Lower third; 1: Upper half of upper third. 2: Lower half of upper third, 3: Upper half of middle third, 4: Lower half of middle third, 5: Upper half of lower third, 6: Lower half of lower third.</p

Histological findings of undifferentiated gastric cancer after <i>H</i>. <i>pylori</i> eradication.

Histological findings of undifferentiated gastric cancer after H. pylori eradication.</p

S2 File -

Background/AimsAlthough undifferentiated gastric cancer (UGC) diagnosed after Helicobacter pylori eradication (HPE) carries a poor prognosis, characteristics of post-HPE UGC have not been evaluated in detail because of its low incidence. Therefore, we compared the clinicopathologic characteristics of UGC and differentiated gastric cancers (DGC) diagnosed after successful HPE.MethodsGC lesions from patients who had successfully completed HPE and who had undergone upper gastrointestinal endoscopy between January 2004 and March 2016 were analyzed. Tumors were divided into DGC and UGC groups. Clinicopathologic factors of background and tumor characteristics were compared using univariate and multiple logistic analyses.ResultsA total of 129 tumors from 115 patients were evaluated; 113 tumors were in the DGC group and 16 in the UGC group. Depressed-type tumors (P = 0.024) and sub-submucosal invasion (PPP = 0.047) and absent follow-up (OR 4.99, 95%CI:1.60–15.57; P = 0.006) were significant independent risk factors for UGC. The DGC group showed a gradually decreasing temporal trend by trend test (P = 0.015), while the UGC group showed a relatively constant incidence over time, although the number of cases was small.ConclusionUGC was diagnosed even after long time spans following HPE, although the number of cases was small. Female sex, and especially absent follow-up, were risks for post-HPE UGC, suggesting that diligent long-term follow-up after HPE is essential.</div

Distribution of intra-tumor histologic types of the UGC group.

Several tumors featured different gastric cancer histologic types according to the Japanese Classification of Gastric Carcinoma (English version is 3rd 2011, October 2017).</p