1 research outputs found
Screening and partial purification of antifungal metabolite from <i style="mso-bidi-font-style:normal">Streptomyces rochei</i> MSA14: an isolate from marine mining soil of Southwest coast of India.
888-897A total of
fourteen actinobacterial strains were isolated from the mining sediment of
Manavalakurichi, Southeast coast of India. Primary screening results
through agar well diffusion method revealed that 28.57% actinobacterial strains
had in vitro antifungal activity. Most potent actinobacterial isolate MSA14
showed strongest inhibitory activity and was identified as Streptomyces rochei
through morphological, physiological, biochemical and 16S rRNA gene sequence
characteristics. Crude ethyl acetate extract of S. rochei exhibited wide
spectrum antifungal activity which was ranged between 12 and 17 mm. Further
evaluation of Minimum inhibitory concentration (MIC) and Minimum fungicidal
concentration (MFC) showed the values ranged from 50 to 200 and 100 to 200
µg/ml, respectively. Partial purification of crude extract through TLC using
various gradient solvent system recorded different spots of active principles
with the respective Rf values between 0.22 and 0.90. TLC autobiography assay
evidenced that, spot with the Rf value of 0.54 had promising antagonistic
activity