1 research outputs found
<i>Bacillus subtilis</i> Biosensor Engineered To Assess Meat Spoilage
Here,
we developed a cell-based biosensor that can assess meat
freshness using the Gram-positive model bacterium <i>Bacillus
subtilis</i> as a chassis. Using transcriptome analysis, we identified
promoters that are specifically activated by volatiles released from
spoiled meat. The most strongly activated promoter was P<i><sub>sboA</sub></i>, which drives expression of the genes required
for the bacteriocin subtilosin. Next, we created a novel BioBrick
compatible integration plasmid for <i>B. subtilis</i> and
cloned P<i><sub>sboA</sub></i> as a BioBrick in front of
the gene encoding the chromoprotein amilGFP inside this vector. We
show that the newly identified promoter could efficiently drive fluorescent
protein production in <i>B. subtilis</i> in response to
spoiled meat and thus can be used as a biosensor to detect meat spoilage