Confocal laser scanning microscopy of the urinary bladder.

<p><i>In vivo</i> confocal laser scanning microscopy of the bladder showing eggs of <i>Schistosoma haematobium</i> (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0034869#pone-0034869-g001" target="_blank">figure 1a and 1b</a>) with their typical terminal spine (arrow) (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0034869#pone-0034869-g001" target="_blank">figure 1b</a>).</p

Preservation of egg morphology after incubation with different fixatives and KOH concentrations.

<p>Incubation of 70% ethanol-fixed placental tissue (A) or naive placental tissue (B) and infected mouse liver with 4% KOH for 24h at 37°C. Mouse liver pieces incubated alone with 4% KOH (C) or 10% KOH (D) served as controls. In all groups incubated with 4% KOH the eggshells of nearly all eggs of <i>S</i>. <i>mansoni</i> were intact and the lateral spine was easy to identify. The eggs contained a more or less developed miracidium or were without any content. <i>S</i>. <i>mansoni</i> eggs in the 10% KOH control group were nearly completely digested. The morphology and the lateral spine were hard to identify.</p