Koncentracija laktoferina u mlijeku krava tijekom involucije mliječne žlijezde s različitim bakteriološkim nalazima.

Lactoferrin is an iron-binding glycoprotein of the transferrin family, present in high concentrations in secretions from the mammary glands during the involution period, and has antimicrobial ability. To determine lactoferrin concentrations in bovine milk with different bacteriological findings, 151 quarter milk samples were collected on a dairy farm of the Holstein-Friesian breed in Vojvodina, Republic of Serbia. Classical microbiological methods were used for bacteria isolation, and ELISA analysis was used for lactoferrin concentration quantification. The most common isolated bacteria in bovine milk samples were Corynebacterium spp. (32.45%) and coagulase-negative staphylococci (4.64%) with lactoferrin concentrations of 6.0497 ± 1.6774 mg/mL and 5.2961 ± 1.3633 mg/mL, respectively. The lowest mean value of lactoferrin concentration was observed in uninfected quarters and quarters infected with environmental pathogens, while the highest concentration of lactoferrin was in udder quarters infected with Streptococcus agalactiae. One in four milk samples where Staphylococcus aureus was isolated had much lower lactoferrin concentrations (1.1736 mg/mL) than the other three samples (6.2089 ± 0.5016 mg/mL), which requires further research.Laktoferin, glikoprotein koji veže željezo, pripadnik porodice bjelančevina transferin, prisutan je u visokoj koncentraciji u sekretu mliječne žlijezde tijekom involucije i posjeduje antimikrobnu sposobnost. Radi utvrđivanja koncentracije laktoferina u mlijeku krava s različitim bakteriološkim nalazima, prikupljen je 151 pojedinačni uzorak mlijeka na farmi holštajn-frizijske pasmine na području Autonomne pokrajine Vojvodine Republike Srbije. Za identifikaciju bakterija korištene su klasične mikrobiološke metode i ELISA za određivanje koncentracije laktoferina. Najčešće izdvojene bakterije u uzorcima mlijeka krava bile su Corynebacterium spp. (32,45%) s koncentracijom laktoferina 6,0497 ± 1,6774 mg/mL i koagulaza negativni stafilokoki (4,64%) s koncentracijom laktoferina 5,2961 ± 1,3633 mg/mL. Najniža srednja vrijednost koncentracije laktoferina zabilježena je u negativnim četvrtima vimena i četvrtima inficiranim bakterijama iz okoliša, dok je najviša koncentracija laktoferina bila u četvrtima vimena inficiranima vrstom Streptococcus agalactiae. Jedan od četiriju uzoraka mlijeka gdje je bio izdvojen Staphylococcus aureus imao je znatno nižu vrijednost laktoferina (1,1736 mg/mL) od ostala tri uzorka (6,2089 ± 0,5016 mg/mL), što zahtijeva daljnja istraživanja

Prevalencija gena za rezistenciju na meticilin i Panton-Valentine leukocidin u izolatima bakterije Staphylococcus aureus podrijetlom od goveda i ljudi.

Using the California Mastitis Test (CMT), on 46 highly productive dairy farms in Serbia, cows with milk secretion disorder were identified. Milk samples were taken from cows with positive CMT and from cows with clinical mastitis. Standard microbiological analysis of milk samples and API Staph confirmed the presence of 75 isolates of Staphylococcus aureus. Those 75 isolates, as well as 11 isolates of Staphylococcus aureus originating from humans were analyzed for the presence of genes encoding Panton-Valentine leukocidin (PVL) and PBP2A protein, responsible for methicillin resistance. The presence of gene encoding PVL was determined by PCR in 5 out of 75 (6.67%) and in 7 out of 11 (63.63%) bovine and human isolates of Staphylococcus aureus, respectively. The presence of the mecA gene was determined by PCR in 1 of 75 (1.33%) and in 2 of 11 (18.18%) bovine and human isolates of Staphylococcus aureus, respectively. The presence of the mecC gene was not determined in analyzed isolates. Further research is needed to investigate the genetic relationship between bovine and human Staphylococcus aureus isolates, to determine the exact impact of bovine Staphylococcus aureus strains from the cow udders on animal and public health.Uporabom kalifornijskog testa za upalu vimena na 46 visoko produktivnih mliječnih farmi u Srbiji identificirane su krave s poremećajem sekrecije mlijeka. Uzeti su uzorci mlijeka krava pozitivnih kalifornijskim testom i krava s kliničkim mastitisom. Standardnom mikrobiološkom pretragom uzoraka mlijeka i identifikacijom kompletom API Staph potvrđena je prisutnost 75 izolata bakterije Staphylococcus aureus. Tih 75 izolata, kao i 11 izolata te bakterije podrijetlom iz ljudi bili su analizirani lančanom reakcijom polimerazom na prisutnost gena koji kodiraju za Panton-Valentine leukocidin (PVL) i PBP2A protein odgovoran za meticilinsku rezistenciju. Prisutnost gena koji kodira za PVL dokazana je u pet od 75 (6,67%) izolata podrijetlom iz goveda i u sedam od 11 (63,63%) izolata podrijetlom iz ljudi. Prisutnost mecA gena dokazana je u jednog od 75 (1,33%) izolata podrijetlom iz goveda i u dva od 11 (18,18%) podrijetlom iz ljudi. Prisutnost mecC gena nije utvrđena u analiziranim izolatima. Potrebna su daljnja istraživanja kako bi se istražio odnos između izolata Staphylococcus aureus-a podrijetlom iz ljudi i iz goveda i odredio točan utjecaj sojeva vrste Staphylococcus aureus iz vimena krava na zdravlje životinja i zdravlje ljudi