Prevalencija gena za rezistenciju na meticilin i Panton-Valentine leukocidin u izolatima bakterije Staphylococcus aureus podrijetlom od goveda i ljudi.

Abstract

Using the California Mastitis Test (CMT), on 46 highly productive dairy farms in Serbia, cows with milk secretion disorder were identified. Milk samples were taken from cows with positive CMT and from cows with clinical mastitis. Standard microbiological analysis of milk samples and API Staph confirmed the presence of 75 isolates of Staphylococcus aureus. Those 75 isolates, as well as 11 isolates of Staphylococcus aureus originating from humans were analyzed for the presence of genes encoding Panton-Valentine leukocidin (PVL) and PBP2A protein, responsible for methicillin resistance. The presence of gene encoding PVL was determined by PCR in 5 out of 75 (6.67%) and in 7 out of 11 (63.63%) bovine and human isolates of Staphylococcus aureus, respectively. The presence of the mecA gene was determined by PCR in 1 of 75 (1.33%) and in 2 of 11 (18.18%) bovine and human isolates of Staphylococcus aureus, respectively. The presence of the mecC gene was not determined in analyzed isolates. Further research is needed to investigate the genetic relationship between bovine and human Staphylococcus aureus isolates, to determine the exact impact of bovine Staphylococcus aureus strains from the cow udders on animal and public health.Uporabom kalifornijskog testa za upalu vimena na 46 visoko produktivnih mliječnih farmi u Srbiji identificirane su krave s poremećajem sekrecije mlijeka. Uzeti su uzorci mlijeka krava pozitivnih kalifornijskim testom i krava s kliničkim mastitisom. Standardnom mikrobiološkom pretragom uzoraka mlijeka i identifikacijom kompletom API Staph potvrđena je prisutnost 75 izolata bakterije Staphylococcus aureus. Tih 75 izolata, kao i 11 izolata te bakterije podrijetlom iz ljudi bili su analizirani lančanom reakcijom polimerazom na prisutnost gena koji kodiraju za Panton-Valentine leukocidin (PVL) i PBP2A protein odgovoran za meticilinsku rezistenciju. Prisutnost gena koji kodira za PVL dokazana je u pet od 75 (6,67%) izolata podrijetlom iz goveda i u sedam od 11 (63,63%) izolata podrijetlom iz ljudi. Prisutnost mecA gena dokazana je u jednog od 75 (1,33%) izolata podrijetlom iz goveda i u dva od 11 (18,18%) podrijetlom iz ljudi. Prisutnost mecC gena nije utvrđena u analiziranim izolatima. Potrebna su daljnja istraživanja kako bi se istražio odnos između izolata Staphylococcus aureus-a podrijetlom iz ljudi i iz goveda i odredio točan utjecaj sojeva vrste Staphylococcus aureus iz vimena krava na zdravlje životinja i zdravlje ljudi