Evaluation of the Hamilton City Council plants for Gullies programme

This evaluation found that the Hamilton City Council Plants for Gullies programme is successfully facilitating the restoration and enhancement of Hamilton City gullies by private gully owners. The mean number of native species in surveyed gullies was 2.1 in non-restored sites and 18.4 in restored sites. While the mean number of invasive species was 4.1 in non-restored sites to 2.6 in restored sites. This quantitative measure is a valuable indication of the ecosystem gains for Hamilton City. Hamilton gully owners are very satisfied with the Plants for Gullies programme; the mean satisfaction rating was 8.9 out of 10. These residents dedicate significant time and energy to restoring their gully sections; the mean time contribution of survey participants was 10.3 hours per month. Gully owners were found to be utilising knowledge acquired through participation in the programme to add valuable diversity to their gully ecosystems. This was repeatedly demonstrated by programme participants not only reintroducing the native plants supplied by the programme but also adding large quantities of privately-sourced plants. This investigation found that the Plants for Gullies and Gully Restoration programmes are effective in communicating key ecological restoration concepts. This was reflected by gully owner prioritisation of eco-sourcing, biodiversity and weed control as considerations in their restoration projects. The Gully Restoration Guide was found to be the most valuable component of the programme’s educational tools. However, it is recommended that this resource is updated to support the many gully owners who require information for advanced stages of ecological restoration. In summary, the Plants for Gullies programme is successfully delivering gully restoration assistance and advice to gully owners, which is resulting in significant improvements to Hamilton City’s gully systems. The programme is valued by all who are involved and could be recommended to other New Zealand cities as an effective model for environmental restoration and community engagement

Population Genetics and Autecology of the Endemic Shrub Epiphyte Pittosporum cornifolium

A comprehensive investigation of the population genetics and autecology of the endemic New Zealand shrub epiphyte Pittosporum cornifolium (Pittosporaceae) is presented. Pittosporum cornifolium has a wide geographic range and is well adapted to a variety of lifestyles namely terrestrial, rupestral, and more commonly, epiphytic. The primary habitats of P. cornifolium are lowland and coastal ecosystems which, in recent times (<200 years) have been subjected to widespread clearance and fragmentation resulting in major reductions to the species potential population range. The study focused on five populations in the North Island from Coromandel to Taranaki where habitat loss of lowland and coastal ecosystems has been significant. Population-level genetic analysis using Inter-Simple Sequence Repeat (ISSR) markers revealed that while P. cornifolium exhibited high genetic diversity at the species-level, genetic diversity was relatively low at the population-level. The outcrossing dioecious breeding system and unique evolutionary history of P. cornifolium are likely to be key factors influencing the observed high intra-specific diversity whereas reduced genetic diversity at population-level is probably due to geographic isolation caused by recent habitat fragmentation. Ecological parameters were investigated to determine the current ecological status of the five populations and results did not reveal any substantial ecological impediments to regeneration and dispersal modes. Ecological data were incorporated with information from national data sets to provide a more comprehensive overview of P. cornifolium autecology and to develop a predicted environmental distribution map. Key findings indicate P. cornifolium is typically affiliated with old growth forest systems and well drained low nutrient substrates, while low mean daily temperatures (<0.6°C) restrict environmental distribution. Both genetic and autecological research was applied to determine levels of intra-specific divergence in cultivated P. cornifolium individuals from the Poor Knights Islands (outer Hauraki Gulf), which are morphologically distinct from mainland forms. The Poor Knights Islands individuals were the most genetically distinct as revealed by ISSR analysis, having higher pairwise levels of genetic distance than iii mainland populations as well as more unique loci. A single mutation in the sequence of the Internal Transcribed Spacer (ITS) region was revealed in the Poor Knights Islands individuals, distinguishing them from mainland P. cornifolium and additional members of a monophyletic clade which have shared ITS sequences. Furthermore, P. cornifolium from the Poor Knights Islands have significant morphological and anatomical differences such as larger leaves and leaf tissue depths. Long term isolation on the offshore islands is likely to have had the most significant effect on this population divergence. The differences in the Poor Knights Islands individuals may warrant the delineation of a new subspecies or even species. However, a more comprehensive examination of the taxon across its mainland range, the Poor Knights Island group, and other northern offshore islands where the species is present is recommended to clarify current inferences. The results of this research have provided a framework for the development of species specific conservation and restoration strategies for P. cornifolium and reveal the importance of provenance and microhabitat (lifestyle) when sourcing seed for reintroduction projects

Are current ecological restoration practices capturing natural levels of genetic diversity? A New Zealand case study using AFLP and ISSR data from mahoe (Melicytus ramiflorus)

Sourcing plant species of local provenance (eco-sourcing) has become standard practice in plant community restoration projects. Along with established ecological restoration practices, knowledge of genetic variation in existing and restored forest fragments is important for ensuring the maintenance of natural levels of genetic variation and connectivity (gene flow) among populations. The application of restoration genetics often employs anonymous ‘fingerprinting’ markers in combination with limited sample sizes due to financial constraints. Here, we used two such marker systems, AFLPs and ISSRs, to estimate population-level genetic variation of a frequently used species in restoration projects in New Zealand, māhoe (Melicytus ramiflorus, Violaceae). We examined two rural and two urban forest fragments, as potential local source populations, to determine whether the māhoe population at the recently (re)constructed ecosystem at Waiwhakareke Natural Heritage Park (WNHP), Hamilton, New Zealand reflects the genetic variation observed in these four potential source populations. Both marker systems produced similar results and indicated, even with small population sizes, that levels of genetic variation at WNHP were comparable to in situ populations. However, the AFLPs did provide finer resolution of the population genetic structure than ISSRs. ISSRs, which are less expensive and technically less demanding to generate than AFLPs, may be sufficient for restoration projects where only a broad level of genotypic resolution is required. We recommend the use of AFLPs when species with a high conservation status are being used due to the greater resolution of this technique

Ecological compensation: an evaluation of regulatory compliance in New Zealand

Ecological compensation is an example of a trade-off whereby loss of natural values is remedied or offset by a corresponding compensatory action on the same site or elsewhere, determined through the process of Environmental Impact Assessment (EIA). Ecological compensation actions are often criticised for having low levels of compliance: meaning that they are achieved only partially or not at all, while development activity proceeds with much greater certainty. Our research investigated compliance with 245 conditions relating to ecological compensation across 81 case studies across New Zealand under the Resource Management Act 1991. Our results show that present tools and practice in New Zealand are not adequately securing the necessary benefits from ecological compensation requirements, with 35.2% of requirements not being achieved. Significant variation in non-compliance with ecological compensation occurs between different activities, applicant types and condition types, while critical variables within the planning process influence levels of compliance. Our research demonstrates the importance of understanding the nature of non-compliance and of providing a consistent and robust decision-making framework for the consideration of ecological compensation in practic

Interventions for preventing oral mucositis for patients with cancer receiving treatment

Background: Treatment of cancer with chemotherapy is becoming increasingly more effective but is associated with short and long-term side effects. Oral side effects remain a major source of illness despite the use of a variety of agents to prevent them. Objectives: To evaluate the effectiveness of oral (and topical) prophylactic agents for oral mucositis and oral candidiasis in patients with cancer (excluding head and neck cancer), compared with placebo or no treatment. Search Strategy: Computerised MEDLINE, EMBASE, CINAHL, CANCERLIT, the Cochrane Controlled Trials Register and the Cochrane Oral Health Group Specialist Register search up to July 1999. Reference lists from relevant articles were scanned and the authors of eligible studies were contacted to identify trials and obtain additional information. Selection Criteria: Studies were selected if they met the following criteria: design - random or quasi-random allocation of participants; participants - anyone with cancer receiving chemotherapy (excluding head and neck cancer); interventions - prophylactic agents prescribed to reduce oral conditions arising from cancer or its treatment; outcomes - mucositis and oral candidiasis. Data Collection and Analysis: Information regarding methods, participants, interventions and outcome measures and results were independently extracted, in duplicate, by two reviewers (JC &amp; HW). Specialist advice was sought to categorise interventions. Authors were contacted for details of randomisation and withdrawals and a quality assessment was carried out using the Jadad criteria (Jadad 1998). The Cochrane Oral Health Group statistical guidelines were followed and relative risk values calculated using random effects models where significant heterogeneity was detected (P &lt; 0.1). Main Results: Thirty-eight reports of trials were initially included. Two were duplicate reports and nine were excluded as there was no useable information. Of the 27 useable studies 14 had data for mucositis comprising 945 randomised patients and 15 included data for oral candidiasis with 1164 randomised patients. Of the eight prophylactic agents used for mucositis only one, ice chips, was effective (Relative risk 0.57, 95% CI 0.43 to 0.77, chi-square for heterogeneity = 0.26 (df = 1), p = 0.61). The NNT to prevent one extra case of mucositis over the baseline incidence using ice chips was 4 (95%CI: 3 to 7). The NNT for when the baseline incidence of mucositis in the population ranges from 50% to 80% are 5 to 4 respectively. There is evidence that antifungal agents which are partially or fully absorbed from the gastrointestinal tract prevent oral candidiasis and that the partially absorbed agents may be more effective than the fully absorbed agents. The RR for partially absorbed agents was 0.13 (95% CI 0.06 to 0.27, chi-square for heterogeneity = 5.3 (df = 3), P = 0. 15). The NNT to prevent one extra case of oral candidiasis over the baseline incidence using partially absorbed drugs was 3 (95% CI: 3 to 5). The NNT for when the baseline incidence of oral candidiasis in the population ranges from 30% to 70% are 4 to 2 respectively. The general reporting of RCT's was poor however the median Jadad score was acceptable and improved further when the authors provided additional information. The sensitivity analysis confirmed the findings for oral candidiasis. Reviewer's Conclusions: There is some evidence that ice chips prevent mucositis. None of the other prophylactic agents included in this review prevented mucositis. There is evidence that prophylactic use of antifungal agents which are absorbed or partially absorbed from the gastrointestinal tract reduce the clinical signs of oral candidiasis, and the partially absorbed drugs may be more effective. Future trials in this area should address the link between oral and general health including outcomes relevant to the patient. Collaboration between medical and dental teams is indicated.</p

Compensating for ecological harm - the state of play in New Zealand

Ecological compensation involves measures to create positive conservation outcomes intended to offset the residual impacts of development (e.g. restoration planting, pest control). Rarely, however, have the exchanges arranged been subject to objective assessment. Here we assess 110 cases of ecological compensation involving diverse New Zealand ecosystems on the basis of how they addressed the six key implementation issues identified by McKenney and Kiesecker (2010: Environmental Management 45: 165–176): equivalence, location (i.e. spatial proximity), additionality, timing, duration and compliance, and currencies. Our research showed that habitat enhancement and protection is the most common form of ecological compensation, and that 72 of 110 case studies undertook compensation on the same site or immediately adjacent. The great majority (94.5%) of compensation was required by condition of resource consent to be demonstrated after the development had proceeded, with an average of 11.3 years of continuing management or monitoring required. The most common form of security other than a consent condition was a covenant (29 of 110 cases) followed by a resource management bond (25). We also found that in 97 cases there was no objective quantification of the compensation needed to make up for impact losses, with the requirements being devised by negotiation between parties with the assistance of expert input. We recognise the potential of ecological compensation as a policy tool, but recommend that significant improvements are made to its implementation to enhance ecological outcomes

Impact of dietary phosphorous in diploid and triploid Atlantic salmon (Salmo salar L.) with reference to early skeletal development in freshwater

In order to assess the effect of dietary phosphorus (P) in reducing vertebral malformations and improving freshwater (FW) performance in triploid Atlantic salmon (Salmo salar), both triploid and diploid Atlantic salmon were fed three different dietary P inclusion levels (low: 4.9, medium: 7.7, and high: 9.7 g available P kg&minus;1) from first feeding until smolt. Somatic and skeletal response was assessed at fry (~0.5 g), parr (~5 g) and smolt (~45 g) stages. Triploid parr initially grew faster on the high P diet, while groups fed low P resulted in a significantly higher weight at smolt. Image analysis of double stained Alcian blue and Alizarin red S fry revealed that low P fed triploid fish presented less well mineralised vertebrae, and significantly more malformed vertebrae in both parr and smolt stages following x-ray radiographic assessment. Triploid parr fed high and medium P had similar numbers of malformed vertebrae relative to their diploid counterparts but greater numbers than at smolt. Low P fed triploids had the highest prevalence of jaw and vertebral malformations as well as the highest number of deformed vertebrae in the central caudal vertebral region, which was more pronounced at parr than at smolt. Shorter vertebrae dorso-ventral lengths were observed throughout the spinal column (R1&ndash;R4) in parr fed low P and only in the caudal region (R3) at smolt. In parr, both ploidies showed reduced phosphate homeostasis protein fgf23 gene expression in vertebrae when fed low P diets, while triploids showed greater down-regulation of osteogenic factors (alp, opn and igf1r) between diets relative to diploids, suggesting possible greater active suppression of mineralisation and reduced osteogenic potential in triploids. No effects of diet or ploidy on gene expression were evident at smolt. Comparisons between development stages suggest early P supplementation in triploids is crucial for skeletal development. Ultimately, reducing vertebral deformities observed at smolt with higher P supplementation in triploids could contribute towards improving skeletal performance and welfare of the stocks in the marine phase

Interventions for the treatment of oral and oropharyngeal cancers:Surgical treatment

Background: Surgery is an important part of the management of oral cavity cancer with regard to both the removal of the primary tumour and removal of lymph nodes in the neck. Surgery is less frequently used in oropharyngeal cancer. Surgery alone may be treatment for early‐stage disease or surgery may be used in combination with radiotherapy, chemotherapy and immunotherapy/biotherapy. There is variation in the recommended timing and extent of surgery in the overall treatment regimens of people with these cancers. This is an update of a review originally published in 2007 and first updated in 2011. Objectives: To determine which surgical treatment modalities for oral and oropharyngeal cancers result in increased overall survival, disease‐free survival and locoregional control and reduced recurrence. To determine the implication of treatment modalities in terms of morbidity, quality of life, costs, hospital days of treatment, complications and harms. Search methods: Cochrane Oral Health's Information Specialist searched the following databases: Cochrane Oral Health's Trials Register (to 20 December 2017), the Cochrane Central Register of Controlled Trials (CENTRAL; 2017, Issue 11), MEDLINE Ovid (1946 to 20 December 2017) and Embase Ovid (1980 to 20 December 2017). We searched the US National Institutes of Health Trials Registry (ClinicalTrials.gov) and the World Health Organization International Clinical Trials Registry Platform for ongoing trials. There were no restrictions on the language or date of publication. Selection criteria: Randomised controlled trials where more than 50% of participants had primary tumours of the oral cavity or oropharynx, or where separate data could be extracted for these participants, and that compared two or more surgical treatment modalities, or surgery versus other treatment modalities. Data collection and analysis: Two or more review authors independently extracted data and assessed risk of bias. We contacted study authors for additional information as required. We collected adverse events data from included studies. Main results: We identified five new trials in this update, bringing the total number of included trials to 12 (2300 participants; 2148 with cancers of the oral cavity). We assessed four trials at high risk of bias, and eight at unclear. None of the included trials compared different surgical approaches for the excision of the primary tumour. We grouped the trials into seven main comparisons. Future research may change the findings as there is only very low‐certainty evidence available for all results. Five trials compared elective neck dissection (ND) with therapeutic (delayed) ND in participants with oral cavity cancer and clinically negative neck nodes, but differences in type of surgery and duration of follow‐up made meta‐analysis inappropriate in most cases. Four of these trials reported overall and disease‐free survival. The meta‐analyses of two trials found no evidence of either intervention leading to greater overall survival (hazard ratio (HR) 0.84, 95% confidence interval (CI) 0.41 to 1.72; 571 participants), or disease‐free survival (HR 0.73, 95% CI 0.25 to 2.11; 571 participants), but one trial found a benefit for elective supraomohyoid ND compared to therapeutic ND in overall survival (RR 0.40, 95% CI 0.19 to 0.84; 67 participants) and disease‐free survival (HR 0.32, 95% CI 0.12 to 0.84; 67 participants). Four individual trials assessed locoregional recurrence, but could not be meta‐analysed; one trial favoured elective ND over therapeutic delayed ND, while the others were inconclusive. Two trials compared elective radical ND with elective selective ND, but we were unable to pool the data for two outcomes. Neither study found evidence of a difference in overall survival or disease‐free survival. A single trial found no evidence of a difference in recurrence. One trial compared surgery plus radiotherapy with radiotherapy alone, but data were unreliable because the trial stopped early and there were multiple protocol violations. One trial comparing positron‐emission tomography‐computed tomography (PET‐CT) following chemoradiotherapy (with ND only if no or incomplete response) versus planned ND (either before or after chemoradiotherapy), showed no evidence of a difference in mortality (HR 0.92, 95% CI 0.65 to 1.31; 564 participants). The trial did not provide usable data for the other outcomes. Three single trials compared: surgery plus adjunctive radiotherapy versus chemoradiotherapy; supraomohyoid ND versus modified radical ND; and super selective ND versus selective ND. There were no useable data from these trials. The reporting of adverse events was poor. Four trials measured adverse events. Only one of the trials reported quality of life as an outcome. Authors' conclusions: Twelve randomised controlled trials evaluated ND surgery in people with oral cavity cancers; however, the evidence available for all comparisons and outcomes is very low certainty, therefore we cannot rely on the findings. The evidence is insufficient to draw conclusions about elective ND of clinically negative neck nodes at the time of removal of the primary tumour compared to therapeutic (delayed) ND. Two trials combined in meta‐analysis suggested there is no difference between these interventions, while one trial (which evaluated elective supraomohyoid ND) found that it may be associated with increased overall and disease‐free survival. One trial found elective ND reduced locoregional recurrence, while three were inconclusive. There is no evidence that radical ND increases overall or disease‐free survival compared to more conservative ND surgery, or that there is a difference in mortality between PET‐CT surveillance following chemoradiotherapy versus planned ND (before or after chemoradiotherapy). Reporting of adverse events in all trials was poor and it was not possible to compare the quality of life of people undergoing different surgical treatments

Extracellular Localisation of the C-Terminus of DDX4 Confirmed by Immunocytochemistry and Fluorescence-Activated Cell Sorting

Putative oogonial stem cells (OSCs) have been isolated by fluorescence-activated cell sorting (FACS) from adult human ovarian tissue using an antibody against DEAD-box helicase 4 (DDX4). DDX4 has been reported to be germ cell specific within the gonads and localised intracellularly. White et al. (2012) hypothesised that the C-terminus of DDX4 is localised on the surface of putative OSCs but is internalised during the process of oogenesis. This hypothesis is controversial since it is assumed that RNA helicases function intracellularly with no extracellular expression. To determine whether the C-terminus of DDX4 could be expressed on the cell surface, we generated a novel expression construct to express full-length DDX4 as a DsRed2 fusion protein with unique C- and N-terminal epitope tags. DDX4 and the C-terminal myc tag were detected at the cell surface by immunocytochemistry and FACS of non-permeabilised human embryonic kidney HEK 293T cells transfected with the DDX4 construct. DDX4 mRNA expression was detected in the DDX4-positive sorted cells by RT-PCR. This study clearly demonstrates that the C-terminus of DDX4 can be expressed on the cell surface despite its lack of a conventional membrane-targeting or secretory sequence. These results validate the use of antibody-based FACS to isolate DDX4-positive putative OSCs