Allelic profiles and sequence types (ST) obtained for the 16 <i>B. quintana</i> isolates and the HROEH DNA extract.

<p>Allelic profiles and sequence types (ST) obtained for the 16 <i>B. quintana</i> isolates and the HROEH DNA extract.</p

Characteristics of the 16 <i>B. quintana</i> isolates studied.

<p>HROEH was a DNA extract from the cardiac valve tissue of a patient with culture-negative endocarditis by <i>B. quintana</i>.</p>1<p>BA, bacillary angiomatosis.</p

Phylogenetic relationship between different <i>B. quintana</i> STs as determined by eBURST.

<p>A clonal complex contains STs that have 8 out of 9 alleles in common. ST5 is assigned as a singleton since it differed in 3–7 alleles from all other STs. The size of the circles relates to the frequency of the corresponding ST, and illustrates that the assigned primary founder of the major clonal complex (ST2) is a common clone.</p

Primers used for the amplification and sequencing of the nine genetic loci evaluated for the <i>B. quintana</i> MLST scheme.

1<p>Corresponding to the complete genome sequence of <i>B. quintana</i> strain Toulouse/copy Uppsala, GenBank accession number BX897700.1.</p

Localization of the 9 genetic loci used in the <i>B. quintana</i> MLST scheme on the chromosome of <i>B. quintana</i>.

<p>The genomic map was adapted from <a href="http://wishart.biology.ualberta.ca/BacMap/graphs_cgview.html" target="_blank">http://wishart.biology.ualberta.ca/BacMap/graphs_cgview.html</a><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0009765#pone.0009765-Stothard1" target="_blank">[32]</a>. The position of the loci has been marked by arrows.</p

Characteristics of the nine loci evaluated for the <i>B. quintana</i> MLST scheme.

<p>Characteristics of the nine loci evaluated for the <i>B. quintana</i> MLST scheme.</p

Neighbour-joining tree of the concatenated sequences of <i>B. henselae</i> STs as reconstructed by MEGA4.

<p>1,000 bootstrap replicates were used to examine the confidence in the tree. The only node to score above 60 is the one leading to Group 1 (75%, as indicated), indicating that the delineation of Group 1 represents a real division in the <i>B. henselae</i> population.</p

Correlation between ST and geographic origin among 182 <i>B. henselae</i> isolates analysed.

a<p>North-western Europe including Denmark, Sweden, UK, the Netherlands, Germany, Czech Republic, France (isolates from Paris and Strasbourg), and Switzerland</p>b<p>Mediterranean region including Italy, Marseille (Urlly8 isolate), and Israel</p>c<p>As determined by chi square test</p>d<p>p values have a relative low reliability because of the small number of isolates in this ST</p

Frequency of feline and human <i>B. henselae</i> isolates within each ST in correlation with the geographic origin of the isolates.

<p>Frequency of feline and human <i>B. henselae</i> isolates within each ST in correlation with the geographic origin of the isolates.</p

Geographic origin, host species and clinical source of <i>B. henselae</i> isolates.

a<p>BA, bacillary angiomatosis</p