Additional file 6: of Production of highly and broad-range specific monoclonal antibodies against hemagglutinin of H5-subtype avian influenza viruses and their differentiation by mass spectrometry

Data from the advanced immunoreactivity studies. Figure S7. The ELISA titration curves of the mAbs against rHA - A/H5N1/Qinghai from a mammalian expression system. Figure S8. The ELISA titration curves of the mAbs against rHA - A/H5N1/Poland from a baculovirus expression system. Figure S9. The ELISA titration curves of the mAbs against rHA - A/H5N1/Poland from a bacterial expression system. Table S11. The concentration values interpolated from the mAb titration curves. Figure S10. Diversity of the mAb reactivities with recombinant H5 hemagglutinin proteins and H5N3 avian influenza virus. Figure S11. Relative reactivity of the mAbs with recombinant H5 hemagglutinin antigens. Figure S12. Relative reactivity of the mAbs with avian influenza viruses. (PDF 422 kb

Relative and absolute light output of the J-PET scintillators as a function of the WLS concentration.

<p>Relative and absolute light output of the J-PET scintillators as a function of the WLS concentration.</p

Emission spectra of BC-420, J-PET scintillator and scintillators’ absorption coefficient <i>μ</i>_<i>eff</i> [37, 38].

<p>The emission spectra are normalized in amplitude.</p

Averaged signals from photomultiplier connected to BC-420 scintillator (top) and to J-PET scintillator (bottom).

<p>Red dashed line indicates result of fitting a function given by the sum of Formulas <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0186728#pone.0186728.e013" target="_blank">(2)</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0186728#pone.0186728.e014" target="_blank">(3)</a>. Signals were normalized to the amplitude equal to 1V.</p

Thermal schedule for scintillator polymerization.

<p>Thermal schedule for scintillator polymerization.</p

Average signals measured with detectors consisting of the BC-420 or the J-PET scintillators and Hamamatsu R9800 photomultiplier.

<p>The shown shapes result from averaging of 10⁵ signals using method described in references [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0186728#pone.0186728.ref017" target="_blank">17</a>]. Signals were normalized to the amplitude equal to 1V.</p

Charge spectra measured with the J-PET and BC-420 scintillator.

<p>PM1, PM2, PM3, PM4 used in the legend denote photomultipliers indicated in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0186728#pone.0186728.g003" target="_blank">Fig 3</a>. In the left side charge spectra of the J-PET scintillator (0.05J-PET or 0.5J-PET) measured with photomultipliers PM1 and PM2 are compared to the spectra of BC-420 scintillator measured with the same pair of photomultipliers. The number in the name of the J-PET scintillator indicates the concentration of WLS in ‰. In the right side spectra of the J-PET scintillators are compared to spectra of BC-420 measured with the same pair of photomultipliers: PM3 and PM4. The difference between the spectra for the low values of charge are due to the fact that PM1 and PM3 were used in the trigger. The differences at large charge (at the Compton edge) are due to the differences in the light yield.</p

Emission spectra of scintillators and quantum efficieny of photomultipliers.

<p>Emission spectra of the J-PET (solid line) and BC-420 (dashed line) scintillators [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0186728#pone.0186728.ref022" target="_blank">22</a>] superimposed on the quantum efficiency dependence on photons wavelength for typical vacuum tube photomultiplier with bialcali window (dotted line) [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0186728#pone.0186728.ref035" target="_blank">35</a>] and silicon photomultipliers (dashed-dotted line) [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0186728#pone.0186728.ref035" target="_blank">35</a>]. Maximum of emission for BC-420 scintillator is placed at wavelength of 393 nm while the maximum of the J-PET scintillator at 403 nm. The emission spectra are normalized in amplitude.</p

Relative and absolute light output of the J-PET plastic scintillators.

<p>Relative and absolute light output of the J-PET plastic scintillators.</p

Properties of J-PET scintillator and of exemplary plastic scintillators manufactured by Saint Gobain [22].

<p>Properties of J-PET scintillator and of exemplary plastic scintillators manufactured by Saint Gobain [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0186728#pone.0186728.ref022" target="_blank">22</a>].</p