Additional file 2: of European trauma guideline compliance assessment: the ETRAUSS study

Haemodynamic and fluid management according to respondent specialty (Table  3 supplemental work). Percentages are calculated on the basis of total number of respondents (‘n’ in each column), except for vasopressors (number of respondents in the Vasopressor row) as 19 gave no answer for this section. SAP systolic arterial pressure, TBI traumatic brain injury, MAP mean arterial pressure. (DOCX 107 kb

Additional file 1: of MALDI-TOF MS monitoring of PBMC activation status in sepsis

Figure S1. Comparison between in vitro and in vivo data. Averaged spectra of PBMCs stimulated in vitro by different agonists were generated from the database using the Biotyper software. The spectra (n = 16) from 2 other patients with gram-negative bacillus bacteremia, the second patients with S. aureus bacteremia and three other patients with undocumented infection were then compared with the averaged spectra of the database. Matching scores between each spectrum from patients and averaged spectra from the database are represented with circles. Horizontal lines represent the medians of matching scores; a value higher than 1.5 was considered significant and allowed confident identification of the activation status of PBMCs. (JPG 320 kb

Granuloma formation according to lymphopenia or monocytopenia.

<p>Granuloma formation with BCG-coated beads (left) and CB-coated beads (right) was measured during 9 days in PBMCs from patients with severe sepsis. Patients were classified according to lymphopenia (A) and monocytopenia (B). The results are expressed as the percentage of beads entirely covered by PBMCs. The boxplots represent the medians with the first and third quartiles. The whiskers represent the highest value that is within 1.5*IQR. Data beyond the end of the whiskers are outliers and plotted as black points. * p < 0.05 represents the differences between patients with and without monocytopenia.</p

Patient features.

<p>Patient features.</p

Additional file 1: of Effects of cyclosporine A pretreatment of deceased organ donors on kidney graft function (Cis-A-rein): study protocol for a randomized controlled trial

SPIRIT 2013 Checklist: recommended items to address in a clinical trial protocol and related documents. (DOC 121 kb

The Practice of Therapeutic Hypothermia after Cardiac Arrest in France: A National Survey

<div><h3>Aims</h3><p>Cardiac arrest is a major health concern worldwide accounting for 375,000 cases per year in Europe with a survival rate of <10%. Therapeutic hypothermia has been shown to improve patients’ neurological outcome and is recommended by scientific societies. Despite these guidelines, different surveys report a heterogeneous application of this treatment. The aim of the present study was to evaluate the clinical practice of therapeutic hypothermia in cardiac arrest patients.</p> <h3>Methods</h3><p>This self-declarative web based survey was proposed to all registered French adult intensive care units (ICUs) (n = 357). Paediatrics and neurosurgery ICUs were excluded. The different questions addressed the structure, the practical modalities of therapeutic hypothermia and the use of prognostic factors in patients admitted after cardiac arrest.</p> <h3>Results</h3><p>One hundred and thirty-two out of 357 ICUs (37%) answered the questionnaire. Adherence to recommendations regarding the targeted temperature and hypothermia duration were 98% and 94% respectively. Both guidelines were followed in 92% ICUs. During therapeutic hypothermia, sedative drugs were given in 99% ICUs, mostly midazolam (77%) and sufentanil (59%). Neuromuscular blocking agents (NMBA) were used in 97% ICUs, mainly cisatracurium (77%). Numerous prognostic factors were used after cardiac arrest such as clinical factors (95%), biomarkers (53%), electroencephalography (78%) and evoked potentials (35%).</p> <h3>Conclusions</h3><p>In France, adherence to recommendations for therapeutic hypothermia after cardiac arrest is higher than those previously reported in other countries. Numerous prognostic factors are widely used even if their reliability remains controversial.</p> </div

Practice of hypothermia target temperature.

<p>Figure sectors in light blue to dark blue are in the recommended target. Figure sectors in light orange to orange are outside the recommended target.</p

Practice of hypothermia duration.

<p>Figure sectors in light blue to dark blue are in the recommended target. Figure sectors in light orange to orange are outside the recommended target.</p

Additional file 1 of OCOSO2: study protocol for a single-blinded, multicentre, randomised controlled trial assessing a central venous oxygen saturation-based goal-directed therapy to reduce postoperative complications in high-risk patients after elective major surgery

Additional file 1