Phytochemical profiles and evaluation of the biological potential of ethyl acetate and <i>n</i>-butanol fractions of the aerial parts of <i>Cistus albidus</i> L

The phytochemical profiles of the ethyl acetate (EAFCA) and n-butanol (BFCA) fractions of the aerial parts of Cistus albidus L., were characterised by a developed LC-ESI-MS/MS method, using 53 fingerprint phytochemicals, revealing the presence of 26 and 22 compounds, respectively with quinic acid, gallic acid, protocatechuic acid, catechin, gentisic acid, tannic acid, ellagic acid, quercitrin, astragalin as the major compounds of the EAFCA, in very higher amounts than in the BFCA. The antioxidant activity was evaluated using six methods (DPPH•, ABTS•+, GOR•, CUPRAC, FRAP and Phenanthroline). In agreement with its highest polyphenolic content (430.12 ± 1.02 µg GAE/mg of extract) and largest amounts of identified polyphenolics, the EAFCA exhibited a higher antioxidant activity than the BFCA. Additionally, the EAFCA showed the highest acetylcholinesterase (AChE) inhibition (IC50 of 25.1 ± 2.58 µg/mL). Moreover, both EAFCA and BFCA demonstrated high photoprotective activity, with sun protection factor (SPF) values of 36.49 and 36.52, respectively.</p