Molecular Detection of Helicobacter pylori by Real Time- PCR in Dyspeptic Patients

Helicobacter pylori  is one of the most common infection worldwide and is associated with gastric disorders. H. pylori is genetically unstable and this reflected on its virulence factors and type of diseases. Cytotoxin associated gene A (CagA) product is a major virulence factor is thought to be associated with gastric diseases. In the present study, we used RT-PCR for rapid detection of H. pylori infection in peptic ulcer patient, the determination of CagA gene directly from gastric biopsy specimens and determining relationship between gastric cancer and H. pylori. Gastric biopsy were collected from 54 patients with disorders in digestive system from AL-Hussein teaching hospital. The results of  current study showed that the  presence of 16SrRNA for H. pylori was 21(38.9%), 15 of 24(62.5%) were males and 6 of 30(20.0%) were females, whereas the percentage of CagA gene was 18(33.3%), 12 of 24 (50%) were males and 6 of 30 (20%) were females by using Real time quantitative PCR in  54 biopsy specimens. These result revealed a significant difference between males and females. Our study indicated that CagA gene RT-PCR is the most specific for the detection of H. pylori in gastric biopsy specimens in patients with gastric cancer compared with 16SrRNA gene . There were significant differences (p≤0.05) in CagA positive rate, among different diseases. In this study the presence of H. pylori in patients with Gastric cancer and peptic ulcer has been demonstrated by using RT-PCR method to support the hypothesis that H. pylori has a place in etiology of gastric cancer

Molecular Detection of Helicobacter pylori by Real Time- PCR in Dyspeptic Patients

Helicobacter pylori  is one of the most common infection worldwide and is associated with gastric disorders. H. pylori is genetically unstable and this reflected on its virulence factors and type of diseases. Cytotoxin associated gene A (CagA) product is a major virulence factor is thought to be associated with gastric diseases. In the present study, we used RT-PCR for rapid detection of H. pylori infection in peptic ulcer patient, the determination of CagA gene directly from gastric biopsy specimens and determining relationship between gastric cancer and H. pylori. Gastric biopsy were collected from 54 patients with disorders in digestive system from AL-Hussein teaching hospital. The results of  current study showed that the  presence of 16SrRNA for H. pylori was 21(38.9%), 15 of 24(62.5%) were males and 6 of 30(20.0%) were females, whereas the percentage of CagA gene was 18(33.3%), 12 of 24 (50%) were males and 6 of 30 (20%) were females by using Real time quantitative PCR in  54 biopsy specimens. These result revealed a significant difference between males and females. Our study indicated that CagA gene RT-PCR is the most specific for the detection of H. pylori in gastric biopsy specimens in patients with gastric cancer compared with 16SrRNA gene . There were significant differences (p?0.05) in CagA positive rate, among different diseases. In this study the presence of H. pylori in patients with Gastric cancer and peptic ulcer has been demonstrated by using RT-PCR method to support the hypothesis that H. pylori has a place in etiology of gastric cancer

I-V Characteristics of ITO/CdTe/Al 2 O 3 /Si/Au Thin Film Solar Cell

Abstract The (1.7, 3.5, and 4.5n

MiRNA-133a y MiRNA-25 3p y su relación con algunas variables en suero de pacientes con Osteoporosis

Osteoporosis is a chronic disease characterized by bone fragility that results in fractures and a variety of miRNAs are involved in osteoclast differentiation therefore, the current case control study aimed to estimate miRNA-133a and miRNA-25 3p in osteoporotic patients and evaluate relationship of these miRNAs with some variables including (calcium, vitamin D, BMD, smoking,  history of previous fracture and gender, this study conducted on fifty patients suffering from osteoporosis with age range between 50-88 years, other group  consist of 45 healthy individuals with an age range between 55-87 years included in this study as a control group. Blood samples used to extraction of miRNA-133a and miRNA-25 3p from the serum of patients and healthy control as a biomarker for osteoporosis were quantitated by using RT-PCR. Results:  miR-133a fold change was significantly upregulated   in serum of   osteoporotic patients and highest in patients group compared with control group, miR-133a highly significant difference in miR-133 among study groups (P < 0.001); while no significant difference in miR-25 among study groups (P = 0.295); although the level of patients groups was higher than that of control group. Receiver operator characteristic (ROC) curve  of  miR-133  was carried out and cutoff  value  was  >8.3  with  sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV)  and area under curve of 76%, 80%,82.6%, 72.7% and 0.815 (0.723- 906), while (ROC) curve analysis and  cutoff  value of miR-25 was >1.32  with  sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV) and Area under curve of 66%, 45%, 60%, 51.4% and 0.565 (0.444- 685).Conclusion: miRNA-133a is high sensitivity and Specificity in this study which was bushed to using them as a biomarker for osteoporosis diagnosis.   La osteoporosis es una enfermedad crónica caracterizada por la fragilidad ósea que resulta en fracturas y una variedad de miARN están involucrados en la diferenciación de los osteoclastos, por lo tanto, el presente estudio de casos y controles tuvo como objetivo estimar miARN-133a y miARN-25 3p en pacientes osteoporóticos y evaluar la relación de estos miARN. con algunas variables que incluyen (calcio, vitamina D, DMO, tabaquismo, antecedentes de fracturas previas y sexo, este estudio se realizó en cincuenta pacientes que padecían osteoporosis con rango de edad entre 50-88 años, otro grupo conformado por 45 individuos sanos con rango de edad entre 55-87 años incluidos en este estudio como grupo control. Las muestras de sangre utilizadas para la extracción de miARN-133a y miARN-25 3p del suero de pacientes y control sano como biomarcador de osteoporosis se cuantificaron mediante RT-PCR. Resultados: el cambio de miR-133a se incrementó significativamente en el suero de pacientes osteoporóticos y fue más alto en el grupo de pacientes en comparación con el grupo de control, miR-133a hi diferencia muy significativa en miR-133 entre los grupos de estudio (P <0,001); mientras que no hubo diferencias significativas en el miR-25 entre los grupos de estudio (P = 0,295); aunque el nivel de los grupos de pacientes fue superior al del grupo de control. Se realizó la curva de características del operador del receptor (ROC) de miR-133 y el valor de corte fue> 8,3 con sensibilidad, especificidad, valor predictivo positivo (VPP), valor predictivo negativo (VPN) y área bajo la curva de 76%, 80%, 82,6 %, 72,7% y 0,815 (0,723-906), mientras que el análisis de la curva (ROC) y el valor de corte de miR-25 fue> 1,32 con sensibilidad, especificidad, valor predictivo positivo (VPP), valor predictivo negativo (VPN) y Área bajo la curva de 66%, 45%, 60%, 51,4% y 0,565 (0,444- 685). Conclusión: el miARN-133a es de alta sensibilidad y especificidad en este estudio que se propuso utilizarlos como biomarcador para el diagnóstico de osteoporosis

Analysis of Docetaxel Adverse Drug Reactions: A Retrospective Study Based on Iraqi Pharmacovigilance Center Database

Docetaxel is an effective treatment approved for many types of cancers, but its effectiveness in clinical practice can be compromised by significant occurrence of adverse drug reactions. The aim of the current study was to measure the distribution of adverse drug reactions of docetaxel reported in Iraq and to assess the causality, severity, seriousness, preventability, expectedness and outcome of these adverse reactions. A retrospective study conducted on individual case safety reports from the Iraqi Pharmacovigilance Center / Ministry of Health. The study included 118 individual case safety report containing 236 adverse drug reactions. Most of the adverse drug reactions were related to skin and subcutaneous tissue disorders(26.7%), followed by respiratory, thoracic and mediastinal disorders (20.8%), gastrointestinal disorders (17.4%) and general disorders and administration site conditions (10.6%). The majority of these reactions with possible causality (68.6%), moderate severity (75.4%), expected (80.5%), possibly preventable (93.2%), and serious (80.5%). In addition the most common outcome of adverse drug reactions was recovered / resolved (46.19%)